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- 검색키워드
- 저자 : Heien, Michael L. (검색결과 3 건)
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Tracking tonic dopamine levels <i>in vivo</i> using multiple cyclic square wave voltammetry
Oh, Yoonbae,Heien, Michael L.,Park, Cheonho,Kang, Yu Min,Kim, Jaekyung,Boschen, Suelen Lucio,Shin, Hojin,Cho, Hyun U.,Blaha, Charles D.,Bennet, Kevin E.,Lee, Han Kyu,Jung, Sung Jun,Kim, In Young,Lee, Elsevier 2018 Biosensors & bioelectronics Vol.121 No.-
<P><B>Abstract</B></P> <P>For over two decades, fast-scan cyclic voltammetry (FSCV) has served as a reliable analytical method for monitoring dopamine release in near real-time <I>in vivo</I>. However, contemporary FSCV techniques have been limited to measure only rapid (on the order of seconds, <I>i.e.</I> phasic) changes in dopamine release evoked by either electrical stimulation or elicited by presentation of behaviorally salient stimuli, and not slower changes in the tonic extracellular levels of dopamine (<I>i.e.</I> basal concentrations). This is because FSCV is inherently a differential method that requires subtraction of prestimulation tonic levels of dopamine to measure phasic changes relative to a zeroed baseline. Here, we describe the development and application of a novel voltammetric technique, multiple cyclic square wave voltammetry (M-CSWV), for analytical quantification of tonic dopamine concentrations <I>in vivo</I> with relatively high temporal resolution (10 s). M-CSWV enriches the electrochemical information by generating two dimensional voltammograms which enable high sensitivity (limit of detection, 0.17 nM) and selectivity against ascorbic acid, and 3,4-dihydroxyphenylacetic acid (DOPAC), including changes in pH. Using M-CSWV, a tonic dopamine concentration of 120 ± 18 nM (n = 7 rats, ± SEM) was determined in the striatum of urethane anethetized rats. Pharmacological treatments to elevate dopamine by selectively inhibiting dopamine reuptake and to reduce DOPAC by inhibition of monoamine oxidase supported the selective detection of dopamine <I>in vivo</I>. Overall, M-CSWV offers a novel voltammetric technique to quantify levels and monitor changes in tonic dopamine concentrations in the brain to further our understanding of the role of dopamine in normal behavior and neuropsychiatric disorders.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Highly sensitive and selective electrochemical method for detecting tonic dopamine level <I>in vivo</I>. </LI> <LI> Enhanced visualization of the data by two-dimensional voltammogram of electrochemical responses. </LI> <LI> High temporal resolution (10 s). </LI> </UL> </P>
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Shin, Hojin,Oh, Yoonbae,Park, Cheonho,Kang, Yumin,Cho, Hyun U.,Blaha, Charles D.,Bennet, Kevin E.,Heien, Michael L.,Kim, In Young,Lee, Kendall H.,Jang, Dong Pyo American Chemical Society 2020 ANALYTICAL CHEMISTRY - Vol.92 No.1
<P>Although N-shaped fast scan cyclic voltammetry (N-FSCV) is well-established as an electroanalytical method to measure extracellular serotonin concentrations <I>in vivo</I>, it is in need of improvement in both sensitivity and selectivity. Based on our previous studies using fast cyclic square-wave voltammetry (FCSWV) for <I>in vivo</I> dopamine measurements, we have modified this technique to optimize the detection of serotonin <I>in vivo</I>. A series of large amplitude square-shaped potentials was superimposed onto an N-shaped waveform to provide cycling through multiple redox reactions within the N-shaped waveform to enhance the sensitivity and selectivity to serotonin measurement when combined with a two-dimensional voltammogram. N-Shaped fast cyclic square-wave voltammetry (N-FCSWV) showed significantly higher sensitivity to serotonin compared to conventional N-FSCV. In addition, N-FCSWV showed better performance than conventional N-shaped FSCV in differentiating serotonin from its major interferents, dopamine and 5-hydroxyindoleascetic acid (5-HIAA). It was also confirmed that the large amplitude of the square waveform did not influence local neuronal activity, and it could monitor electrical stimulation evoked phasic release of serotonin in the rat substantia nigra pars reticulata (SNr) before and after systemic injection of escitalopram (ESCIT, 10 mg/kg i.p.), a serotonin selective reuptake inhibitor.</P> [FIG OMISSION]</BR>
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Fast Cyclic Square-Wave Voltammetry To Enhance Neurotransmitter Selectivity and Sensitivity
Park, Cheonho,Oh, Yoonbae,Shin, Hojin,Kim, Jaekyung,Kang, Yumin,Sim, Jeongeun,Cho, Hyun U.,Lee, Han Kyu,Jung, Sung Jun,Blaha, Charles D.,Bennet, Kevin E.,Heien, Michael L.,Lee, Kendall H.,Kim, In Youn American Chemical Society 2018 ANALYTICAL CHEMISTRY - Vol.90 No.22
<P>Although fast-scan cyclic voltammetry (FSCV) has been widely used for in vivo neurochemical detection, the sensitivity and selectivity of the technique can be further improved. In this study, we develop fast cyclic square-wave voltammetry (FCSWV) as a novel voltammetric technique that combines large-amplitude cyclic square-wave voltammetry (CSWV) with background subtraction. A large-amplitude, square-shaped potential was applied to induce cycling through multiple redox reactions within a square pulse to increase sensitivity and selectivity when combined with a two-dimensional voltammogram. As a result, FCSWV was significantly more sensitive than FSCV (<I>n</I> = 5 electrodes, two-way ANOVA, <I>p</I> = 0.0002). In addition, FCSWV could differentiate dopamine from other catecholamines (e.g., epinephrine and norepinephrine) and serotonin better than conventional FSCV. With the confirmation that FCSWV did not influence local neuronal activity, despite the large amplitude of the square waveform, it could monitor electrically induced phasic changes in dopamine release in rat striatum before and after injecting nomifensine, a dopamine reuptake inhibitor.</P> [FIG OMISSION]</BR>
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