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      • SCIEKCI등재

        Examination of Correlations Between Several Biochemical Components and Powdery Mildew Resistance of Flax Cultivars

        Aly, Aly A.,Mansour, Mahmoud T. M.,Mohamed, Heba I.,Abd-Elsalam, Kamel A. The Korean Society of Plant Pathology 2012 Plant Pathology Journal Vol.28 No.2

        A field trial was conducted in 2009/2010 and 2010/2011 growing seasons at Giza Agricultural Research Station to examine correlations between some biochemical componets and powdery mildews ($PM_s$) resistance in flax cultivars. Nine flax cultivars could be divided into five distinct groups, i.e., highly susceptible (Cortland and C.I. 2008), moderately susceptible (Giza 7, and Marshall), moderately resistant (Cass), resistant (Koto, Dakota and Wilden), and highly resistant (Ottowa 770B). The cultivars showed considerable variation in PM severity ranged from 8.05 on Ottowa 770B to 97.02% on Cortland. Total soluble proteins, total phenols, antioxidant enzymes (peroxidase and polyphenoloxidase), ascorbic acid, tocopherol, and malondialdehyde (MDA), were determined in uninfected leaves of the tested cultivars. Pearson's correlation coefficient was calculated to measure the degree of association between PM severity and each component. All components showed significant (P < 0.05) or highly significant (P < 0.01) negative correlation with PM severity except MDA, which showed positive correlation (P < 0.01). Linear regression analysis was used to evaluate the causal relationship between the biochemical components (independent variables) and PM severity (dependent variable). Coefficient of determination ($R^2$) values of the generated models ranged from 48.76 to 77.15%. Tocopherol, MDA, and proteins were the most important contributors to the total variation in PM severity as the $R^2$ values of their models were 71.78, 75.28, and 77.15%, respectively. The results of the present study suggest that tocopherol, MDA, and proteins in uninfected leaves can be used as biochemical markers to predict PM resistance in flax.

      • KCI등재

        Examination of Correlations Between Several Biochemical Components and Powdery Mildew Resistance of Flax Cultivars

        Aly A. Aly,Kamel A. Abd-Elsalam,Mahmoud T. M. Mansour,Heba I. Mohamed 한국식물병리학회 2012 Plant Pathology Journal Vol.28 No.2

        A field trial was conducted in 2009/2010 and 2010/2011growing seasons at Giza Agricultural Research Station to examine correlations between some biochemical componets and powdery mildews (PMs) resistance in flax cultivars. Nine flax cultivars could be divided into five distinct groups, i.e., highly susceptible (Cortland and C.I. 2008), moderately susceptible (Giza 7, and Marshall),moderately resistant (Cass), resistant (Koto, Dakota and Wilden), and highly resistant (Ottowa 770B). The cultivars showed considerable variation in PM severity ranged from 8.05 on Ottowa 770B to 97.02% on Cortland. Total soluble proteins, total phenols, antioxidant enzymes (peroxidase and polyphenoloxidase), ascorbic acid, tocopherol, and malondialdehyde (MDA), were determined in uninfected leaves of the tested cultivars. Pearson’s correlation coefficient was calculated to measure the degree of association between PM severity and each component. All components showed significant (P < 0.05) or highly significant (P < 0.01) negative correlation with PM severity except MDA, which showed positive correlation (P < 0.01). Linear regression analysis was used to evaluate the causal relationship between the biochemical components (independent variables)and PM severity (dependent variable). Coefficient of determination (R2) values of the generated models ranged from 48.76 to 77.15%. Tocopherol, MDA, and proteins were the most important contributors to the total variation in PM severity as the R2 values of their models were 71.78, 75.28, and 77.15%, respectively. The results of the present study suggest that tocopherol,MDA, and proteins in uninfected leaves can be used as biochemical markers to predict PM resistance in flax.

      • KCI등재

        Kinetics, isotherm, and thermodynamic studies of the adsorption of reactive red 195 A dye from water by modified Switchgrass Biochar adsorbent

        Mohamed E. Mahmoud,Gehan M. Nabil,Nabila M. El-Mallah,Heba I. Bassiouny,Sandeep Kumar,Tarek M. Abdel-Fattah 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.37 No.-

        A method is described for efficient decolorization of reactive red 195 A dye (RR-195A) from aqueous andwastewater samples using modified Switchgrass Biochar (SB) by tetradecyltrimethyl ammoniumbromide (TTAB) as a cationic surfactant for the formation of a novel SB-TTAB adsorbent. The differentcontrolling experimental conditions such as solution pH, initial dye concentration, contact time,adsorbent mass, interfering ions and temperature have been investigated and optimized. Adsorptionequilibrium using Langmuir, Freundlich, Temkin and Dubinin–Radushkevich (D–R) isotherm modelshave been also studied and the best fit was related to the Langmuir adsorption isotherm. Adsorptionkinetics have been tested and illustrated using pseudo first-order, pseudo-second-order, intraparticlediffusion and Elovich models. The collected results confirmed that the pseudo-second order kineticmodel exhibited the best fit with the experimental data. Thermodynamic parameters were calculatedand the results referred to an exothermic nature and spontaneity of the adsorption process. Improveddecolorization of reactive red 195 A dye from textile industrial wastewater and other water sampleswere successfully established. Excellent recovery values of RR-195A dye by SB-TTAB adsorbent weredetermined in the ranges of 96.61–98.76%, 97.79–99.26%, 94.24–100.0% and 90.98–92.96% ( 1.0–4.0%)from tap water, raw water, wastewater and sea water, respectively

      • SCIEKCI등재

        Pathogenicity of Bacillus Strains to Cotton Seedlings and Their Effects on Some Biochemical Components of the Infected Seedlings

        Aly A. Aly,Omima M. El-Mahdy,Marian M. Habeb,Abeer Elhakem,Amal A. Asran,Maryan M. Youssef,Heba I. Mohamed,Rania S. Hanafy 한국식물병리학회 2022 Plant Pathology Journal Vol.38 No.2

        Pathogenicity of eight Bacillus strains to seedlings of four cotton cultivars was evaluated under greenhouse conditions. Each of the tested cultivars was individually treated with powdered inoculum of each bacterial strain. Untreated seeds were planted as control treatments in autoclaved soil. Effects of the tested strains on levels and activities of some biochemical components of the infected seedlings were also assayed. The biochemical components included total soluble sugars, total soluble proteins, total free amino acids, peroxidase, polyphenol oxidase, phenols, and lipid peroxidation. ANOVA showed that Bacillus strain (B) was a very highly significant source of variation in damping-off and dry weight. Cotton cultivar (V) was a nonsignificant source of variation in damping-off while it was a significant source of variation in dry weight. B × V interaction was a significant source of variation in damping- off and a nonsignificant source of variation in dry weight. Bacillus strain was the most important source of variation as it accounted for 59.36 and 64.99% of the explained (model) variation in damping-off and dry weight, respectively. The lack of significant correlation between levels and activities of the assayed biochemical components and incidence of damping-off clearly demonstrated that these biochemical components were not involved in the pathogenicity of the tested strains. Therefore, it was hypothesized that the pathogenicity of the tested strains could be due to the effect of cell wall degrading enzymes of pathogenic toxins. Based on the results of the present study, Bacillus strains should be considered in studying the etiology of cotton seedling damping-off.

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