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      • Morphological Traits of Lotus japonicus (Regal) Ecotypes Collected in Japan

        Hashiguchi, Masatsugu,Tsuruta, Shin-Ichi,Akashi, Ryo Korean Society for Bioinformatics 2011 Interdisciplinary Bio Central (IBC) Vol.3 No.1

        Forty-seven wild accessions of Lotus japonicus Regal (Japanese trefoil) indigenous to Japan were investigated for nine morphological characters. Average temperature and annual precipitation were negatively correlated with stem color and seed weight. On the other hand, latitude was positively correlated with these traits. Consequently, accessions from sites at higher latitudes with low temperatures and precipitation tend to have dark red stems and heavy seeds. Cluster analysis based on nine morphological characters classified 47 wild accessions into six major groups. Cluster I included four accessions of tall and erect plants. These plants are phenotypically similar to commercial variety 'Empire'. Cluster II consisted of three accessions of creep plants with pale red stems. Cluster III contained 24 accessions that had average values for all morphological characters evaluated. Cluster IV included two accessions of erect plants with rounded leaflets and dark red stems. Cluster V included four accessions of small, creep plants with pale red stems. Cluster VI included seven accessions of small and erect plants, a phenotype that also applies to ?Gifu B-129?, which is used as experimental strain worldwide. These data were deposited into LegumeBase, an online database (http://www.legumebase.brc.miyazaki-u.ac.jp/) supported by the National BioResource Project (NBRP) in Japan.

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        Biochemical changes in recombinant inbred lines of soybean (Glycine max L. Merr.) under storage-related stresses

        Jiamtae Pakanan,Hashiguchi Takuyu,Pongpiyapaiboon Sorawich,Lelapiyamit Intouch,Tanaka Hidenori,Hashiguchi Masatsugu,Akashi Ryo 한국작물학회 2023 Journal of crop science and biotechnology Vol.26 No.4

        Soybean is a major crop that is rich in proteins, lipids, and isofavones. Although the seeds are widely used for food and industrial purposes, soybean is recognized as a crop that does not store well, as storage conditions greatly afect seed quality. In this study, we selected 10 recombinant inbred lines (RILs) of soybean that are either sensitive or tolerant to storage, and examined the biochemical characteristics of the lines and their parents under storage-related stresses. The germination rates of all of the lines in the tolerant group were higher than those of the sensitive group for all storage conditions. Malondialdehyde (MDA) content was higher in the sensitive group than in the tolerant group, with the MDA content typically increasing under long-term storage conditions. Superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities were also more afected by long-term storage, but not by higher temperatures. Isofavones were also assayed and it was found that the aglycones (except glycitein) increased with an increase of storage temperature. Malonyl isofavone glucosides tended to decrease under long-term storage and high temperature conditions, suggesting that malonyl glucosides are converted to their aglycones. These results illustrate the mechanisms underlying storage-related stresses and the importance of screening for lines that can tolerate environmental stresses.

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