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- 저자 : Harvey Perez (검색결과 2 건)
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DNA sequence-dependent enhancement of Cy3 fluorescence
Harvey, Billie Jo,Perez, Claudia,Levitus, Marcia Korean Society of Photoscience 2009 Photochemical & photobiological sciences Vol.8 No.8
Cyanine dyes are extensively used as fluorescent probes in molecular biology, biochemical and biophysical applications. We investigated the fluorescent properties of Cy3 covalently attached to the 5' terminus of DNA oligonucleotides, and demonstrated that its fluorescence efficiency and lifetime depend strongly on DNA sequence. DNA sequence determines the extent and nature of the interactions between the dye and the DNA bases, which are responsible for the unusual enhancement in fluorescence observed for a large number of oligonucleotides. Results are discussed in terms of a photoisomerization mechanism that deactivates the excited state and thus competes with fluorescence. The efficiency of isomerization decreases when Cy3-DNA interactions prevent rotation around the double bonds, resulting in an increase in the lifetime of the singlet excited state. We have shown that the ability of Cy3 to interact with DNA depends on the flexibility of the oligonucleotide and the presence of purines in the chain.
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Scaling Insulin-Producing Cells by Multiple Strategies
Eiji Yoshihara,Jinhyuk Choi,Fritz Cayabyab,Harvey Perez 대한내분비학회 2024 Endocrinology and metabolism Vol.39 No.2
In the quest to combat insulin-dependent diabetes mellitus (IDDM), allogenic pancreatic islet cell therapy sourced from deceased donors represents a significant therapeutic advance. However, the applicability of this approach is hampered by donor scarcity and the demand for sustained immunosuppression. Human induced pluripotent stem cells are a game-changing resource for generating synthetic functional insulin-producing β cells. In addition, novel methodologies allow the direct expansion of pancreatic progenitors and mature β cells, thereby circumventing prolonged differentiation. Nevertheless, achieving practical reproducibility and scalability presents a substantial challenge for this technology. As these innovative approaches become more prominent, it is crucial to thoroughly evaluate existing expansion techniques with an emphasis on their optimization and scalability. This manuscript delineates these cutting-edge advancements, offers a critical analysis of the prevailing strategies, and underscores pivotal challenges, including cost-efficiency and logistical issues. Our insights provide a roadmap, elucidating both the promises and the imperatives in harnessing the potential of these cellular therapies for IDDM.
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