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RISS 인기검색어
- 검색키워드
- 저자 : Harold Sun (검색결과 3 건)
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Squid Giant Axon Contains Neurofilament Protein mRNA but does not Synthesize Neurofilament Proteins
Gainer, Harold,House, Shirley,Kim, Dong Sun,Chin, Hemin,Pant, Harish C. Springer-Verlag 2017 Cellular and molecular neurobiology Vol.37 No.3
<P>When isolated squid giant axons are incubated in radioactive amino acids, abundant newly synthesized proteins are found in the axoplasm. These proteins are translated in the adaxonal Schwann cells and subsequently transferred into the giant axon. The question as to whether any de novo protein synthesis occurs in the giant axon itself is difficult to resolve because the small contribution of the proteins possibly synthesized intra-axonally is not easily distinguished from the large amounts of the proteins being supplied from the Schwann cells. In this paper, we reexamine this issue by studying the synthesis of endogenous neurofilament (NF) proteins in the axon. Our laboratory previously showed that NF mRNA and protein are present in the squid giant axon, but not in the surrounding adaxonal glia. Therefore, if the isolated squid axon could be shown to contain newly synthesized NF protein de novo, it could not arise from the adaxonal glia. The results of experiments in this paper show that abundant 3H-labeled NF protein is synthesized in the squid giant fiber lobe containing the giant axon's neuronal cell bodies, but despite the presence of NF mRNA in the giant axon no labeled NF protein is detected in the giant axon. This lends support to the glia-axon protein transfer hypothesis which posits that the squid giant axon obtains newly synthesized protein by Schwann cell transfer and not through intra-axonal protein synthesis, and further suggests that the NF mRNA in the axon is in a translationally repressed state.</P>
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Zhu, Bing-Mei,Kang, Keunsoo,Yu, Ji Hoon,Chen, Weiping,Smith, Harold E.,Lee, Daeyoup,Sun, Hong-Wei,Wei, Lai,Hennighausen, Lothar Oxford University Press 2012 Nucleic acids research Vol.40 No.10
<P>Signal Transducers and Activators of Transcription (STAT) 5A/B regulate cytokine-inducible genes upon binding to GAS motifs. It is not known what percentage of genes with GAS motifs bind to and are regulated by STAT5. Moreover, it is not clear whether genome-wide STAT5 binding is modulated by its concentration. To clarify these issues we established genome-wide STAT5 binding upon growth hormone (GH) stimulation of wild-type (WT) mouse embryonic fibroblasts (MEFs) and MEFs overexpressing STAT5A more than 20-fold. Upon GH stimulation, 23 827 and 111 939 STAT5A binding sites were detected in WT and STAT5A overexpressing MEFs, respectively. 13 278 and 71 561 peaks contained at least one GAS motif. 1586 and 8613 binding sites were located within 2.5 kb of promoter sequences, respectively. Stringent filtering revealed 78 genes in which the promoter/upstream region (−10 kb to +0.5 kb) was recognized by STAT5 both in WT and STAT5 overexpressing MEFs and 347 genes that bound STAT5 only in overexpressing cells. Genome-wide expression analyses identified that the majority of STAT5-bound genes was not under GH control. Up to 40% of STAT5-bound genes were not expressed. For the first time we demonstrate the magnitude of opportunistic genomic STAT5 binding that does not translate into transcriptional activation of neighboring genes.</P>
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Dengfeng Yang,Li’ang Cao,Ce Yang,Dazhong Lao,Harold Sun 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.6
In this work, the influence of thickness and solidity of guide vane on the unsteady flow of a radial inflow turbine with variable nozzle has been numerically studied. Three vanes with the thickness changes from 0.3 to 0.1 and the solidity changes from 1.43 to 2.86, were chosen for this study. By investigating the unsteady flow field, it is found that the vane with low thickness and high solidity (vane B) can reduce shock by 75 % compared to the vane with high thickness and low solidity (base model); meanwhile, it can also mitigate the nozzle endwall leakage flow thus improves the flow uniformity of rotor inlet. As the intensity of shock and nozzle leakage flow were mitigated, the aerodynamic loading fluctuation of rotor blade can be weakened effectively, which will lead to improved rotor blades forced response. However, at small opening, vane B shows about 0.5 % lower efficiency than the base model; at large open condition, the efficiency degradation of vane B is up to 1.4 %. Therefore, there is a trade-off between the efficiency and forced response when choosing the nozzle vane solidity and thickness.
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