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Hajer Naceur Ben Marzoug,Jalloul Bouajila,Monia Ennajar,Ahmed Lebrihi,Florence Mathieu,Franc¸ois Couderc,Manef Abderraba,Mehrez Romdhane 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.4
Essential oils of four different Eucalyptus species (Eucalyptus salubris, Eucalyptus salmonophloia, Eucalyptus oleosa, and Eucalyptus gracilis) grown in southern Tunisia were screened for their antioxidant and antimicrobial properties as well as their chemical compositions. According to gas chromatography-flame ionization detection and gas chromatography-mass spectrometry analysis, chemical compositions of the Eucalyptus species E. salubris (27 compounds; 99.2%), E. salmonophloia (31 compounds; 99.2%), E. oleosa (32 compounds; 97.6%), and E. gracilis (18 compounds; 97.7%) were identified. In the 1,1-diphenyl-2-picrylhydrazyl assay, the antioxidant activity was in the range of 12.0–52.8mg/mL, whereas in the 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonate assay, E. oleosa (176.5±3.1mg/L) gave the best inhibition result. To evaluate antimicrobial activity, all essential oils were tested against bacteria (two Gram-positive and two Gram-negative), two yeast, and two fungi. Essential oils exhibited an interesting antibacterial activity against all microorganisms tested (activity was better against Gram-positive bacteria) except for Staphylococcus aureus and Escherichia coli. Correlations between chemical composition and biological and antioxidant activities were studied.
Naziha Chabir,Mehrez Romdhane,Alexis Valentin,Be´atrice Moukarzel,Hajer Naceur Ben Marzoug,Nadia Ben Brahim,Mohamed Mars,Jalloul Bouajila 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.11
This study investigated the chemical composition (by using gas chromatography/flame ionization detection and gas chromatography/mass spectrometry, an antioxidant [1,1-diphenyl-2-picrylhydrazyl] [DPPH] radical–scavenging assay, and a 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonate [ABTS] radical cation–scavenging assay) and the antimalarial and cytotoxic activities of essential oil extracted from leaves of Melaleuca armillaris. Thirty-two components representing more than 98% of the total composition of the essential oil were identified. The main components were 1,8-cineole (85.8%), camphene (5.05%), and α-pinene (1.95%). The antioxidant activity by ABTS assay showed a mean (±standard deviation) 50% inhibitory concentration (IC50) value of 247.3±3.9 mg/L, and the DPPH assay yielded an IC_50 value of 2183.6±44.3 mg/L. The antimalarial study indicated that the essential oil had mild activity against the chloroquine-resistant Plasmodium falciparum FcB1 strain (IC_50, 27±2 mg/L). The cytotoxic activity of this essential oil was tested against MCF7 human breast cancer cells and was found to be high (IC_50, 12±1 mg/L).