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- 저자 : Haibo Long (검색결과 5 건)
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( Haibo Yuan ),( Yanfeng Liu ),( Xueqin Lv ),( Jianghua Li ),( Guocheng Du ),( Zhongping Shi ),( Long Liu ) 한국미생물 · 생명공학회 2018 Journal of microbiology and biotechnology Vol.28 No.12
The compound 2,5-furandicarboxylic acid (FDCA), an important bio-based monomer for the production of various polymers, can be obtained from 5-hydroxymethylfurfural (HMF). However, efficient production of FDCA from HMF via biocatalysis has not been well studied. In this study, we report the identification of key genes that are involved in FDCA synthesis and then the engineering of Raoultella ornithinolytica BF60 for biocatalytic oxidation of HMF to FDCA using its resting cells. Specifically, previously unknown candidate genes, adhP3 and alkR, which were responsible for the reduction of HMF to the undesired product 2,5- bis(hydroxymethyl)furan (HMF alcohol), were identified by transcriptomic analysis. Combinatorial deletion of these two genes resulted in 85.7% reduction in HMF alcohol formation and 23.7% improvement in FDCA production (242.0 mM). Subsequently, an aldehyde dehydrogenase, AldH, which was responsible for the oxidation of the intermediate 5-formyl-2-furoic acid (FFA) to FDCA, was identified and characterized. Finally, FDCA production was further improved by overexpressing AldH, resulting in a 96.2% yield of 264.7 mM FDCA. Importantly, the identification of these key genes not only contributes to our understanding of the FDCA synthesis pathway in R. ornithinolytica BF60 but also allows for improved FDCA production efficiency. Moreover, this work is likely to provide a valuable reference for producing other furanic chemicals.
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Distributed Finite-time Regulation of a Class of Networked Heterogeneous Multi-agent Systems
Yuan Liu,Shicheng Wang,Haibo Min,Long Ma,Dacheng Luo 제어·로봇·시스템학회 2015 International Journal of Control, Automation, and Vol.13 No.1
In this paper, we investigate the finite-time regulation problem for heterogeneous multiagent system composed of linear first-order, second-order integrator agents and nonlinear Euler- Lagrange (EL) dynamical agents. Based on homogeneity approach, a continuous finite-time controller is designed. A distinctive feature of this work is to address the finite-time consensus problem of firstorder, second-order integrator agents and nonlinear EL agents in a unified theoretical framework. Simulation results are also provided to illustrate the effectiveness of the controller.
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Xiuping Han,Dongyan Chen,Dehui Chen,Haibo Long 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.4
A closed-loop supply chain system consisting of one manufacturer with random yield and one retailer with random demand is discussed in this paper. The models of expected profit of supply chain system are founded under centralized decision and decentralized decision, respectively. The optimal strategies of yield and order are obtained in two modes. It is proved that the order of retailer is not affected by yield randomness. That is showed by numerical example, the results show: the scheduled production of new product for manufacture will decrease and the total profit and each participant of supply chain will increase as recovery price increases, when the cost of remanufacturing production is lower than the new product.
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Preparation of nano aluminum nitride powders by polymer network method
Ning Zhang,Tingting Fu,Feiyi Yang,Hongmin Kan,Xiaoyang Wang,Haibo Long,Liuyan Wang 한양대학교 세라믹연구소 2014 Journal of Ceramic Processing Research Vol.15 No.2
In this work, aluminum nitrate (Al(NO3)3), active carbon and C12H22O11 were used as raw materials. The nano aluminum nitride powders (AlN) were synthesis by polymer network method. Aluminum nitride (AlN) exhibits high thermal conductivity, low dielectric permittivity and good electric insulativity. It has a very high synthetic temperature, however, results in a high cost. The aim of this investigation is to develop a new method of fabricating ultra-fine AlN powders to lower the synthetic temperature of AlN. The influences of different carbon sources and the synthetic temperature on fabricating AlN powders via polymer network method were investigated, and the mechanism of AlN formation was also identified. The results indicated that as using sucrose (C12H22O11) as the carbon source, the optimum synthetic process condition for fabricating ultrafine AlN powders was at 1500 °C for 2 h. Comparing with the mechanical ball-milling method, the temperature of synthesizing AlN powders was lowered about 100 °C. Furthermore, the size of as-synthesized AlN was finer from 50 nm to 80 nm.
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CDH17 nanobodies facilitate rapid imaging of gastric cancer and efficient delivery of immunotoxin
Jingbo Ma,Xiaolong Xu,Chunjin Fu,Peng Xia,Ming Tian,Liuhai Zheng,Kun Chen,Xiaolian Liu,Yilei Li,Le Yu,Qinchang Zhu,Yangyang Yu,Rongrong Fan,Haibo Jiang,Zhifen Li,Chuanbin Yang,Chengchao Xu,Ying Long,J 한국생체재료학회 2022 생체재료학회지 Vol.26 No.4
Background: It is highly desirable to develop new therapeutic strategies for gastric cancer given the low survival rate despite improvement in the past decades. Cadherin 17 (CDH17) is a membrane protein highly expressed in cancers of digestive system. Nanobody represents a novel antibody format for cancer targeted imaging and drug delivery. Nanobody targeting CHD17 as an imaging probe and a delivery vehicle of toxin remains to be explored for its theragnostic potential in gastric cancer. Methods: Naïve nanobody phage library was screened against CDH17 Domain 1-3 and identified nanobodies were extensively characterized with various assays. Nanobodies labeled with imaging probe were tested in vitro and in vivo for gastric cancer detection. A CDH17 Nanobody fused with toxin PE38 was evaluated for gastric cancer inhibition in vitro and in vivo. Results: Two nanobodies (A1 and E8) against human CDH17 with high affinity and high specificity were successfully obtained. These nanobodies could specifically bind to CDH17 protein and CDH17-positive gastric cancer cells. E8 nanobody as a lead was extensively determined for tumor imaging and drug delivery. It could efficiently co-localize with CDH17-positive gastric cancer cells in zebrafish embryos and rapidly visualize the tumor mass in mice within 3 h when conjugated with imaging dyes. E8 nanobody fused with toxin PE38 showed excellent anti-tumor effect and remarkably improved the mice survival in cell-derived (CDX) and patient-derived xenograft (PDX) models. The immunotoxin also enhanced the anti-tumor effect of clinical drug 5-Fluorouracil. Conclusions: The study presents a novel imaging and drug delivery strategy by targeting CDH17. CDH17 nanobodybased immunotoxin is potentially a promising therapeutic modality for clinical translation against gastric cancer.
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