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Increased Binding Efficiency of Enzyme Complex Based on Biomimicry Technology of Microorganisms
Habin SUN,JeongEun HYEON 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Protein-protein interaction (PPI) has been studied in many ways and from many perspectives, leading to advances in biological functional control. This has also led to the study of scaffold protein, which have a very important role as regulators in signalling, and the development of extracellular multi-enzyme complexes using weak interactions, such as ionic bonding. Here we studied the manufacture of other artificial scaffolding proteins to replace this scaffold protein, and in the process we used two peptides and performed overlap PCR to bind the two DNA. This experiment, which is changed in a typical PCR, allows the primer to be configured to have a 5’ overhang complementary at the end of another ㏖ecule so that the DNA after annealing is expanded. When the two DNA ㏖ecules are expanded in this way, they are mixed and a PCR is carried out with only the primers for the far ends. In the course of the study, two peptide-linked genes were obtained, which produced covalent bond-based proteins that could replace conventional non-covalent bonds. This will bring structural stability to the enzyme complex, providing benefits that do not change easily in the heat.