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RISS 인기검색어
- 검색키워드
- 저자 : Gyana Ranjan Rout (검색결과 3 건)
- 무료
- 기관 내 무료
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Genetic Relationships among 23 Ficus Accessions Using Inter-Simple Sequence Repeat Markers
Rout, Gyana Ranjan,Aparajita, Subhashree 한국작물학회 2009 Journal of crop science and biotechnology Vol.12 No.2
Ficus genus is widely distributed in all the climatic stages and great diversity. The exploration of genetic diversity is a pre-requisite for genome organization in the wild species and the related domesticated ones. Inter-Simple Sequence Repeat (ISSR) markers were used to assess the identification of 23 important Ficus accessions and determination of the genetic relationships among these accessions. Out of 21 ISSR primers tested, five primers produced 116 detectable fragments, of which 106 (91.3%) were polymorphic across the accessions. Each of the five primers produced a fingerprint profile unique to each of the accessions studied, and thus could be solely used for their identification. Thirteen unique bands specific to nine species were detected. These may be converted into species-specific probes for identification purposes. Genetic relationships among these accessions were evaluated by generating a similarity matrix based on the Dice coefficient and the Unweighted Pair Group Method with Arithmetic Average (UPGMA) dendogram. The results showed a clear cut separation of the 23 Ficus accessions and were in broad agreement with the morphology. Both molecular and morphological markers will be useful for preservation of the Ficus germplasm.
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Genetic Relationships among 23 Ficus Accessions Using Inter- Simple Sequence Repeat Markers
Gyana Ranjan Rout,Subhashree Aparajita 한국작물학회 2009 Journal of crop science and biotechnology Vol.12 No.2
Ficus genus is widely distributed in all the climatic stages and great diversity. The exploration of genetic diversity is a pre-requisite for genome organization in the wild species and the related domesticated ones. Inter-Simple Sequence Repeat (ISSR) markers were used to assess the identification of 23 important Ficus accessions and determination of the genetic relationships among these accessions. Out of 21 ISSR primers tested, five primers produced 116 detectable fragments, of which 106 (91.3%) were polymorphic across the accessions. Each of the five primers produced a fingerprint profile unique to each of the accessions studied, and thus could be solely used for their identification. Thirteen unique bands specific to nine species were detected. These may be converted into species-specific probes for identification purposes. Genetic relationships among these accessions were evaluated by generating a similarity matrix based on the Dice coefficient and the Unweighted Pair Group Method with Arithmetic Average (UPGMA) dendogram. The results showed a clear cut separation of the 23 Ficus accessions and were in broad agreement with the morphology. Both molecular and morphological markers will be useful for preservation of the Ficus germplasm. Ficus genus is widely distributed in all the climatic stages and great diversity. The exploration of genetic diversity is a pre-requisite for genome organization in the wild species and the related domesticated ones. Inter-Simple Sequence Repeat (ISSR) markers were used to assess the identification of 23 important Ficus accessions and determination of the genetic relationships among these accessions. Out of 21 ISSR primers tested, five primers produced 116 detectable fragments, of which 106 (91.3%) were polymorphic across the accessions. Each of the five primers produced a fingerprint profile unique to each of the accessions studied, and thus could be solely used for their identification. Thirteen unique bands specific to nine species were detected. These may be converted into species-specific probes for identification purposes. Genetic relationships among these accessions were evaluated by generating a similarity matrix based on the Dice coefficient and the Unweighted Pair Group Method with Arithmetic Average (UPGMA) dendogram. The results showed a clear cut separation of the 23 Ficus accessions and were in broad agreement with the morphology. Both molecular and morphological markers will be useful for preservation of the Ficus germplasm.
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Assessment of Seventy-Three Rice Germplasm by Using Simple Sequence Repeats Markers
Subhashree Nayak,Jadhao Kundansingh Rajpalsingh,Debendra Nath Bastia,Kailash Chandra Samal,Gyana Ranjan Rout 한국작물학회 2014 Journal of crop science and biotechnology Vol.17 No.4
Genetic information of germplasm is the initial requirement for crop breeding programs. Rice is one of the oldest domesticatedcrop species endowed with rich genetic diversity which accounts for over 100,000 landraces and improved cultivars. The aim of thepresent study was to evaluate the genetic assessment of rice germplasm originating from India, the Philippines, China, and Malaysiathrough simple sequence repeat (SSR) markers. About 64 alleles were produced over 24 SSR primer amplifications over the wholegenome of rice. The number of alleles ranged from 1 to 4 with an average of 2.67. Out of 64 amplified bands, 58 bands were polymorphicand 6 were monomorphic bands. Most of the primers showed high polymorphic information content (PIC). The PIC valueranged from 0.53 to 0.87. The cluster analysis indicates that the 73 varieties originating from India, the Philippines, China, andMalaysia were grouped separately and made two major clusters (seven groups). Among the two major clusters, one cluster had 18genotypes which originated from the Phillipines, and China with 29% similarity with other varieties originating from India andMalaysia. Further, it was divided into two subgroups which had eight genotypes and the other had 10 genotypes with 41% similarityamong themselves. All 10 genotypes were international varieties suitable for cultivation in medium land ecosystems. The secondmajor cluster had 55 varieties including commercial rice varieties originating from India and Malaysia. The genotype ‘Swarna’ and‘Manaswini’ had 76% similarity with each other and 69% similarity with the ‘Bhanja’ & ‘Ghanteswari’ which might be the genomeassociation. The second major cluster had 55 genotypes divided into two minor groups. The first group had one genotype, i.e. ‘IR63141-B-18-B’ with 34% similarity with the other 54 genotypes. The second minor group (54 genotypes) again was divided into twogroups; one group had five genotypes with 51% similarity. Another group had 49 genotypes divided into two sub-minor groups. Based on this study, the larger range of similarity values using SSR markers provides greater confidence for the assessment of geneticrelationships among the varieties. These genotypes are suitable for cultivation in upland ecosystems. The information obtainedfrom the SSR profile helps to identify the variety diagnostic markers in 73 rice germplasm accessions. The intra- and inter-variationmight be useful for breeders to improve the rice varieties through selective breeding and cross breeding programs
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