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- 저자 : Greg Johnson (검색결과 3 건)
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Kubota, Akane,Ito, Shogo,Shim, Jae Sung,Johnson, Richard S.,Song, Yong Hun,Breton, Ghislain,Goralogia, Greg S.,Kwon, Michael S.,Laboy Cintró,n, Dianne,Koyama, Tomotsugu,Ohme-Takagi, Masaru,Prune Public Library of Science 2017 PLoS genetics Vol.13 No.6
<▼1><P>Photoperiod is one of the most reliable environmental cues for plants to regulate flowering timing. In <I>Arabidopsis thaliana</I>, CONSTANS (CO) transcription factor plays a central role in regulating photoperiodic flowering. In contrast to posttranslational regulation of CO protein, still little was known about <I>CO</I> transcriptional regulation. Here we show that the CINCINNATA (CIN) clade of class II TEOSINTE BRANCHED 1/ CYCLOIDEA/ PROLIFERATING CELL NUCLEAR ANTIGEN FACTOR (TCP) proteins act as <I>CO</I> activators. Our yeast one-hybrid analysis revealed that class II CIN-TCPs, including TCP4, bind to the <I>CO</I> promoter. TCP4 induces <I>CO</I> expression around dusk by directly associating with the <I>CO</I> promoter <I>in vivo</I>. In addition, TCP4 binds to another flowering regulator, GIGANTEA (GI), in the nucleus, and induces <I>CO</I> expression in a <I>GI</I>-dependent manner. The physical association of TCP4 with the <I>CO</I> promoter was reduced in the <I>gi</I> mutant, suggesting that GI may enhance the DNA-binding ability of TCP4. Our tandem affinity purification coupled with mass spectrometry (TAP-MS) analysis identified all class II CIN-TCPs as the components of the <I>in vivo</I> TCP4 complex, and the <I>gi</I> mutant did not alter the composition of the TCP4 complex. Taken together, our results demonstrate a novel function of CIN-TCPs as photoperiodic flowering regulators, which may contribute to coordinating plant development with flowering regulation.</P></▼1><▼2><P><B>Author summary</B></P><P>For plant adaptation to seasonal environments, a crucial developmental event is flowering, as proper timing of flowering affects reproductive success. Although plants monitor various environmental parameters to optimize this timing, photoperiod information is important for plants to regulate seasonal flowering time, because changes in photoperiod occur in a predictable manner throughout the year. The model plant <I>Arabidopsis thaliana</I> responds to photoperiodic changes and flowers under long-day conditions. Based on genetic analyses using mutants defective in the photoperiodic flowering response, we learned that the transcription factor referred to as CONSTANS (CO) plays a central role in regulating the timing of flowering by directly controlling the expression of florigen (flowering-inducing substrate) gene. Long-day afternoon expression of <I>CO</I> is critical for this regulation; however, we had limited knowledge of <I>CO</I> transcriptional regulation. Here we identified that a group of plant-specific transcription factors belonging to the <I>TCP</I> gene family function as novel <I>CO</I> transcriptional activators. We demonstrated that TCP transcription factors regulate <I>CO</I> transcription together with known regulators of <I>CO</I>. Our results imply that plants utilize multiple transcription factors to precisely coordinate the expression of the key regulator gene, <I>CO</I>, which will directly affect flowering time.</P></▼2>
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Benjamin Maier,Mia Lenling,Hwasung Yeom,Greg Johnson,Stuart Maloy,Kumar Sridharan 한국원자력학회 2019 Nuclear Engineering and Technology Vol.51 No.4
A novel fabrication method of oxide dispersion strengthened (ODS) steel cladding tubes for advanced fastreactors has been investigated using the cold spray powder-based materials deposition process. Coldspraying has the potential advantage for rapidly fabricating ODS cladding tubes in comparison with theconventional multi-step extrusion process. A gas atomized spherical 14YWT (Fe-14%Cr, 3%W, 0.4%Ti, 0.2%Y, 0.01%O) powder was sprayed on a rotating cylindrical 6061-T6 aluminum mandrel using nitrogen asthe propellant gas. The powder lacked the oxygen content needed to precipitate the nanoclusters in ODSsteel, therefore this work was intended to serve as a proof-of-concept study to demonstrate that freestandingsteel cladding tubes with prototypical ODS composition could be manufactured using thecold spray process. The spray process produced an approximately 1-mm thick, dense 14YWT deposit onthe aluminum-alloy tube. After surface polishing of the 14YWT deposit to obtain desired claddingthickness and surface roughness, the aluminum-alloy mandrel was dissolved in an alkaline medium toleave behind a free-standing ODS tube. The as-fabricated cladding tube was annealed at 1000 C for 1 hin an argon atmosphere to improve the overall mechanical properties of the cladding
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Bazer, Fuller W.,Song, Gwonhwa,Kim, Jinyoung,Erikson, David W.,Johnson, Greg A.,Burghardt, Robert C.,Gao, Haijun,Carey Satterfield, M.,Spencer, Thomas E.,Wu, Guoyao Elsevier 2012 Molecular and cellular endocrinology Vol.354 No.1
<P><B>Highlights</B></P><P>► Uterine epithelia secrete proteins and transport nutrients for conceptus development. ► Arginine and secreted phosphoprotein 1 (SPP1) activate MTOR cell signaling. ► Arginine metabolism to nitric oxide and polyamines stimulates conceptus growth. ► SPP1 induces focal adhesions between trophectoderm and uterine epithelia. ► Arginine and SPP1 stimulate conceptus development, implantation and pregnancy.</P> <P><B>Abstract</B></P><P>Morphological differentiation of uterine glands in mammals is a postnatal event vulnerable to adverse effects of endocrine disruptors. Exposure of ewe lambs to a progestin from birth to postnatal day 56 prevents development of uterine glands and, as adults, the ewes are unable to exhibit estrous cycles or maintain pregnancy. Uterine epithelia secrete proteins and transport nutrients into the uterine lumen necessary for conceptus development, pregnancy recognition signaling and implantation, including arginine and secreted phosphoprotein 1 (SPP1). Arginine can be metabolized to nitric oxide and to polyamines or act directly to activate MTOR cell signaling to stimulate proliferation, migration, and mRNA translation in trophectoderm cells. SPP1 binds αvβ3 and α5β1 integrins and induces focal adhesion assembly, adhesion and migration of conceptus trophectoderm cells during implantation. Thus, arginine and SPP1 mediate growth, migration, cytoskeletal remodeling and adhesion of trophectoderm essential for pregnancy recognition signaling and implantation.</P>
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