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      • Adenovirus-mediated Expression of Both Antisense Ornithine Decarboxylase and S-adenosylmethionine Decarboxylase Induces G<sub>1</sub> Arrest in HT-29 Cells

        Gong, Lei,Jiang, Chunying,Zhang, Bing,Hu, Haiyan,Wang, Wei,Liu, Xianxi Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.6

        To evaluated the effect of recombinant adenovirus Ad-ODC-AdoMetDCas which can simultaneously express both antisense ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC) on cell cycle distribution in colorectal cancer cell and investigated underlying regulatory responses, human colorectal cancer cells HT-29 were cultured in RPMI 1640 medium and infected with Ad-ODC-AdoMetDCas. Cell cycle progression was detected by flow cytometry analysis. The expression levels of cell cycle regulated proteins were measured by Western blot analysis. The mRNA level of cyclin D1 was measured by RT-PCR. And a luciferase reporter plasmid of cyclin D1 promoter was constructed to observe the effect of Ad-ODC-AdoMetDCas on cyclin D1 promoter activity. The results showed that recombinant adenovirus Ad-ODC-AdoMetDCas significantly induced $G_1$ arrest, decreased levels of cyclin D1 protein and mRNA and suppressed the promoter activity. Ad-ODC-AdoMetDCas also inhibited nuclear translocation of $\beta$-catenin. In conclusion, downregulation of ODC and AdoMetDC mediated by Ad-ODC-AdoMetDCas transfection induces $G_1$ arrest in HT-29 cells and the arrest was associated with suppression of cyclin D1 expression and inhibition of $\beta$-catenin nuclear translocation. As a new anticancer reagent, the recombinant adenovirus Ad-ODC-AdoMetDCas holds promising hope for the therapy of colorectal cancers.

      • KCI등재

        Ringing Frequency Extraction Method Based on EMD and FFT for Health Monitoring of Power Transistors

        Lei Ren,Chunying Gong 전력전자학회 2019 JOURNAL OF POWER ELECTRONICS Vol.19 No.1

        Condition monitoring has been recognized as an effective and low-cost method to enhance the reliability and improve themaintainability of power electronic converters. In power electronic converters, high-frequency oscillation occurs during theswitching transients of power transistors, which is known as ringing. The ringing frequency mainly depends on the values of theparasitic capacitance and stray inductance in the oscillation loop. Although circuit stray inductance is an important factor thatleads to the ringing, it does not change with transistor aging. A shift in either the inside inductance or junction capacitance is animportant failure precursor for power transistors. Therefore, ringing frequency can be used to monitor the health of powertransistors. However, the switching actions of power transistors usually result in a dynamic behavior that can generate oscillationsignals mixed with background noise, which makes it hard to directly extract the ringing frequency. A frequency extractionmethod based on empirical mode decomposition (EMD) and Fast Fourier transformation (FFT) is proposed in this paper. Theproposed method is simple and has a high precision. Simulation results are given to verify the ringing analysis and experimentalresults are given to verify the effectiveness of the proposed method.

      • SCIESCOPUSKCI등재

        Ringing Frequency Extraction Method Based on EMD and FFT for Health Monitoring of Power Transistors

        Ren, Lei,Gong, Chunying The Korean Institute of Power Electronics 2019 JOURNAL OF POWER ELECTRONICS Vol.19 No.1

        Condition monitoring has been recognized as an effective and low-cost method to enhance the reliability and improve the maintainability of power electronic converters. In power electronic converters, high-frequency oscillation occurs during the switching transients of power transistors, which is known as ringing. The ringing frequency mainly depends on the values of the parasitic capacitance and stray inductance in the oscillation loop. Although circuit stray inductance is an important factor that leads to the ringing, it does not change with transistor aging. A shift in either the inside inductance or junction capacitance is an important failure precursor for power transistors. Therefore, ringing frequency can be used to monitor the health of power transistors. However, the switching actions of power transistors usually result in a dynamic behavior that can generate oscillation signals mixed with background noise, which makes it hard to directly extract the ringing frequency. A frequency extraction method based on empirical mode decomposition (EMD) and Fast Fourier transformation (FFT) is proposed in this paper. The proposed method is simple and has a high precision. Simulation results are given to verify the ringing analysis and experimental results are given to verify the effectiveness of the proposed method.

      • Modeling and Analysis of a Buck/Boost Bidirectional Converter with Developed PWM Switch Model

        Wang Jianhua,Zhang Fanghua,Gong Chunying,Chen Ran 전력전자학회 2011 ICPE(ISPE)논문집 Vol.2011 No.5

        The aim of this paper is to provide a general method for bidirectional converter simulation and evaluation. A developed PWM switch model is proposed for bidirectional power flow application. A typical buck/boost bidirectional converter is chosen as a test bench for evaluation. Small signal analysis results show mode transition between battery charging and discharging modes. It provides a fast simulation tool to investigate steady and transient response of converters. The accuracy of the modeling technique is verified by a detailed comparison of simulated results with the developed PWM switch model and component circuit.

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