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- 저자 : Gogoi Dip K (검색결과 3 건)
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Role of Riboflavin in Induced Resistance against Fusarium Wilt and Charcoal Rot Diseases of Chickpea
Ratul Saikia,yadav mukesh kumar,Saju Varghese,Bhim Pratap Singh,Dip K Gogoi,Rakesh Kumar,Dilip K Arora 한국식물병리학회 2006 Plant Pathology Journal Vol.22 No.4
Riboflavin caused induction of systemic resistance inchickpea against Fusarium wilt and charcoal rotdiseases. The dose effect of 0.01 to 20 mM riboflavinshowed that 1.0 mM concentration was sufficient formaximum induction of resistance; higher concentrationdid not increase the effect. At this concentration,riboflavin neither caused cell death of the host plant nordirectly affected the pathogen’s growth. In time courseobservation, it was observed that riboflavin treatedchickpea plants were inducing resistance 2 days aftertreatment and reached its maximum level from 5 to 7days and then decreased. Riboflavin had no effect onsalicylic acid (SA) levels in chickpea, however, riboflavininduced plants found accumulation of phenolsand a greater activities of phenylalanine ammonia lyase(PAL) and pathogenesis related (PR) protein, peroxidasewas observed in induced plant than the control. Riboflavin pre-treated plants challenged with thepathogens exhibited maximum activity of the peroxidases4 days after treatment. Molecular weight of thepurified peroxidase was 42 kDa. From these studies wedemonstrated that riboflavin induced resistance is PRproteinmediated but is independent of salicylic acid.
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Role of Riboflavin in Induced Resistance against Fusarium Wilt and Charcoal Rot Diseases of Chickpea
Saikia Ratul,Yadav Mukesh,Varghese Saju,Singh Bhim Pratap,Gogoi Dip K,Kumar Rakesh,Arora Dilip K The Korean Society of Plant Pathology 2006 Plant Pathology Journal Vol.22 No.4
Riboflavin caused induction of systemic resistance in chickpea against Fusarium wilt and charcoal rot diseases. The dose effect of 0.01 to 20 mM riboflavin showed that 1.0 mM concentration was sufficient for maximum induction of resistance; higher concentration did not increase the effect. At this concentration, riboflavin neither caused cell death of the host plant nor directly affected the pathogen's growth. In time course observation, it was observed that riboflavin treated chickpea plants were inducing resistance 2 days after treatment and reached its maximum level from 5 to 7 days and then decreased. Riboflavin had no effect on salicylic acid(SA) levels in chickpea, however, riboflavin induced plants found accumulation of phenols and a greater activities of phenylalanine ammonia lyase(PAL) and pathogenesis related(PR) protein, peroxidase was observed in induced plant than the control. Riboflavin pre-treated plants challenged with the pathogens exhibited maximum activity of the peroxidases 4 days after treatment. Molecular weight of the purified peroxidase was 42 kDa. From these studies we demonstrated that riboflavin induced resistance is PR-protein mediated but is independent of salicylic acid.
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Pinky Moni Bhuyan,Sosanka Protim Sandilya,Pranab Kumar Nath,Sakshi Gandotra,Sabtharishi Subramanian,Devid Kardong,Dip Kumar Gogoi 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.4
Mature larvae of Antheraea assamensis were collected from different locations of Assam to isolate the cellulolytic gut microflora. Altogether sixty cellulase degrading bacteria were isolated on agar plates containing microcrystalline cellulose as the sole carbon source. Among them, ten isolates showed hydrolyzing zone on agar plates containing carboxy methyl cellulose (CMC) after staining with Congo-red. Isolate MGB05 exhibited the highest CMCase activity (0.262 U/mL) at 72 h of incubation under submerged condition. FPase and β-glucosidase activity were 0.012 U/mL and 3.71 U/mL respectively. It showed maximum FPase (0.022 U/mL) activity on the 3rd day of incubation in the media containing wheat bran as a carbon source. β-glucosidase production was also found to be highest with wheat bran (20.03 U/mL) at 48 h of incubation. The optimum pH and temperature of FPase activity of MGB05 were found at 6.0 and 50 °C respectively while for β-glucosidase activity, it was maximum at pH 6.0 under 50 °C. In addition, metal ion Mg ++ and Ca ++ enhanced FPase activity up to 110.92% (0.026 U/mL) and 105.31% (0.025 U/mL) respectively. In-vitro antimicrobial bioassay of the most potent cellulolytic bacteria (MGB05) also showed high antimicrobial activity against Escherichia coli (2.9 cm) and Pseudomonas aeruginosa (3.0 cm). The isolate MGB05 has been identified based on 16S rDNA homology as Bacillus pumilus MGB05 with accession KP298708.2. Results encompass the prospective beneficial role of gutmicroflora on digestion and disease resistance, which might be a potential probiotic component to enhance silk productivity.
KCI
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