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RISS 인기검색어
- 검색키워드
- 저자 : Ghovvati, S. (검색결과 2 건)
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Hosseini, Arezoo,Gopalan, Vinod,Nassiri, Mohammadreza,Ghaffarzadehgan, Kamran,Aslaminejad, Ali,Ghovvati, Shahrokh,Smith, Robert A.,Lam, Alfred K.Y. Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20
Estrogen receptor alpha ($ER{\alpha}$) is one of the major sub-types of estrogen receptors. $ER{\alpha}$ plays an important role in cellular proliferation and differentiation, chiefly in mammary tissues. In the present study we aimed to quantify of $ER{\alpha}$ mRNA and protein expression in breast tissues from the Iranian population using a real-time PCR assay. Twenty nine breast tissues including 19 adenocarcinomas and 10 normal controls were recruited from the Iranian population. mRNA extraction and cDNA synthesis were performed from these tissues using commercial kits. $ER{\alpha}$ mRNA and protein expression was quantified using real-time PCR and immunohistochemistry respectively. The results showed high expression of $ER{\alpha}$ mRNA (68%) and protein (53%) in the majority of breast cancer tissues compared to normal breast tissues (p= 0.035). Also, high $ER{\alpha}$ mRNA was associated with tumour size of breast carcinomas. In this study, we first reported the expression of $ER{\alpha}$ in Iranian patients with breast cancers and demonstrated prevalence of the expression to be similar to breast cancers noted in other populations.
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Valizadeh, R.,Behgar, M.,Mirzaee, M.,Naserian, A.A.,Vakili, A.R.,Ghovvati, S. Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.10
This study was conducted to evaluate the effects of the particle size (PS) of alfalfa hay (AH) and soybean hull (SH) on milk production of dairy cows and the population of major cellulolytic bacteria in the rumen. Eight lactating Holstein cows, averaging $590{\pm}33\;kg$ BW and $47{\pm}13$ days in milk (DIM), were assigned in a $4{\times}4$ Latin square design to a $2{\times}2$ factorial arrangement of treatments: alfalfa hay particle size (fine vs. coarse) combined with soy hull (zero or substituted as 50% of AH). The cows were fed diets formulated according to NRC (2001). Physically effective factor (pef) and physically effective fiber (peNDF) contents of diets increased by increasing AH particle size and inclusion of SH in the diets (p<0.01). Dry matter intake was not significantly affected by treatments but intake of peNDF was increased marginally by increasing the PS of AH (p = 0.08) and by SH inclusion (p<0.01) in the diets. Milk production was increased by feeding diets containing SH (p = 0.04), but it was not affected by the dietary PS. Milk fat content was increased by increasing AH particle size (p = 0.03) and decreased by SH substitution for a portion of AH (p<0.01). The numbers of total bacteria and cellulolytic species were not affected by PS of AH or by SH. F. succinogenes was the most abundant species in the rumen followed by R. albus and R. flavefaciens (p<0.01). This study showed that SH cannot replace the physically effective fiber in AH having either coarse or fine particle size. In diets containing SH, increasing of diet PS using coarse AH can maintain milk fat content similar to diets without SH. Particle size and peNDF content of diets did not affect the number of total or fibrolytic bacteria in the rumen.
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