A Case of Inflammatory Metastatic Carcinoma of the Breast

Cho, C. G Korean Dermatological Association 1998 Annals of Dermatology Vol.10 No.1

TRIM72 negatively regulates myogenesis via targeting insulin receptor substrate-1

Lee, C S,Yi, J-S,Jung, S-Y,Kim, B-W,Lee, N-R,Choo, H-J,Jang, S-Y,Han, J,Chi, S-G,Park, M,Lee, J-H,Ko, Y-G Macmillan Publishers Limited 2010 CELL DEATH AND DIFFERENTIATION Vol.17 No.8

Lipid rafts have been known to be platforms to initiate cellular signal transduction of insulin-like growth factor (IGF) inducing skeletal muscle differentiation and hypertrophy. Here, tripartite motif 72 (TRIM72), with a really interesting new gene (RING)-finger domain, a B-box, two coiled-coil domains, and a SPRY (SPla and RYanodine receptor) domain, was revealed to be predominantly expressed in the sarcolemma lipid rafts of skeletal and cardiac muscles. Adenoviral TRIM72 overexpression prevented but RNAi-mediated TRIM72 silencing enhanced C2C12 myogenesis by modulating the IGF-induced insulin receptor substrate-1 (IRS-1) activation through the molecular association of TRIM72 with IRS-1. Furthermore, myogenic activity was highly enhanced with increased IGF-induced Akt activation in the satellite cells of TRIM72<SUP>−/−</SUP> mice, compared to those of TRIM72<SUP>+/+</SUP> mice. Because TRIM72 promoter analysis shows that two proximal E-boxes in TRIM72 promoter were essential for MyoD- and Akt-dependent TRIM72 transcription, we can conclude that TRIM72 is a novel antagonist of IRS-1, and is essential as a negative regulator of IGF-induced muscle differentiation.

Geoacoustic provinces of the South Sea shelf off Korea

Kim, D.C.,Kim, G.Y.,Yi, H.I.,Seo, Y.K.,Lee, G.S.,Jung, J.H.,Chang Kim, J. Pergamon Press 2012 QUATERNARY INTERNATIONAL Vol.263 No.-

In order to characterize geoacoustic provinces of the South Sea shelf of Korea, sediment texture, physical, and geoacoustic properties (p-wave velocity) were measured and calculated based on approximately 350 core samples collected from the study area. The empirical relationship between mean grain size and velocity suggested by Hamilton was used for calculation of p-wave velocity. The inner shelf sediments of the South Sea are characterized by Holocene muddy sediments derived from nearby rivers (i.e., mostly supplied from Nakdong and Seomjin rivers near the Korean shore), whereas the outer shelf areas are dominated by Quaternary sediments (e.g., relict sediments, due to a rising of sea level during the Quaternary) depending on seafloor bathymetry. In this study, the seafloor of the South Sea shelf is differentiated into 4 provinces based on sediment properties (esp. mean grain size and velocity). Province I consisted of muddy sediments affected directly by the Nakdong and Seomjin Rivers discharge. Province II was covered by the sediments transported from two rivers. Province III is marked by mixed Holocene fine materials and coarse Pleistocene materials. Province IV is dominated by relict sediments characterized by Pleistocene coarse sandy materials. Mean grain size, velocity, and wet bulk density increase from Province I (>7.4o, 1515 m/s, and 1.52 g/cm<SUP>3</SUP>, respectively) through Province II (5.4o, 1597 m/s, and 1.69 g/cm<SUP>3</SUP>) to Province III (2.9o, 1766 m/s, and 2.0 g/cm<SUP>3</SUP>). Porosity, on the other hand, exhibits an opposite trend, decreasing from Province I (71.3%) to Province II (60.3%) and decreasing significantly in Province III (44.6%). Province IV is characterized by the highest velocity (1979 m/s average) and the coarsest material (2.1o). Nevertheless the values of physical properties such as wet bulk density, porosity, and density lie between Province II and III.

Phase III Clinical Trial for Immunogenecity and Safety of a New Recombinant Hepatitis B Virus Vaccine (HG-II) in the Newborn

Cho, Y. K 大韓臨床藥理學會 1996 臨床藥理學會誌 Vol.4 No.2

Induction of Hairy Roots and Characterization of Peroxidase Expression as a Potential Root Growth Marker in Sesame

Chun, J. -A.,Lee, J. -W.,Yi, Y. -B.,Park, G. -Y.,Chung, C. -H. Taylor Francis 2009 PREPARATIVE BIOCHEMISTRY AND BIOTECHNOLOGY Vol.39 No.4

<P> Using hypocotyl and cotyledon of sesame seedlings, hairy root cultures were established and cDNA coding for a peroxidase was cloned from the roots. The frequency of sesame hairy root formation was higher in hypocotyl (33.4%) than cotyledon (9.3%). Applicable levels of kanamycin and hygromycin as a selectable marker were 100 µg/mL and 30 µg/mL, respectively. The peroxidase cDNA showed relatively high sequence identity with and similarity to plant class III peroxidase family. The cDNA encoded polypeptide was identified with the presence of three sequence features: 1) the putative 4 disulfide bridges, 2) an ER-targeted signal sequence in the N-terminus, and 3) two triplets, NXS for glycosylation. A real-time RT-PCR exhibited an abrupt increase in the peroxidase transcription activity after 4-week cultures of the sesame hairy roots and its highest level in 6-week cultured hairy roots. In contrast, the growth pattern of sesame hairy roots showed a typical sigmoidal curve. The active hairy root growth began after 2-week culture and their stationary growth phase occurred after 5-week culture. These results suggested that the peroxidase expression patterns at its transcription level could be used a potential indicator signaling a message that there will be no longer active growth in hairy root cultures. The sesame peroxidase gene was differentially expressed in different tissues.</P>

A compound (DW1182v) protecting high glucose/palmitate-induced glucolipotoxicity to INS-1 beta cells preserves islet integrity and improves hyperglycemia in obese db/db mouse

Lee, S.J.,Choi, S.E.,Hwang, Y.C.,Jung, I.R.,Yi, S.A.,Jung, J.G.,Ku, J.M.,Jeoung, K.,Han, S.J.,Kim, H.J.,Kim, D.J.,Lee, K.W.,Kang, Y. North-Holland ; Elsevier Science Ltd 2012 european journal of pharmacology Vol.696 No.1

Loss of beta cells is a pathogenic cause for the development of type 2 diabetes. High glucose/free fatty acid (HG/FFA)-induced glucolipotoxicity was thought to play a role in the beta cell loss. Thus, application of small molecules capable of preventing HG/FFA-induced glucolipotoxicty to beta cells could be an avenue for a therapeutic intervention for the development of type 2 diabetes. We screened a representative library supplied from Korean Chemical Bank for prevention of high glucose/palmitate (HG/PA)-induced viability reduction of INS-1 beta cells and were able to identify a new small molecule (DW1182v) with a function to protect HG/PA-induced glucolipotoxicity. The protective effect was specific to HG/PA-induced beta cell death since DW1182v did not protect streptozotocin- or cytokine-induced INS-1 cell death. The protective effect by DW1182v was likely due to the reduction of death-promoting endoplasmic reticulum (ER) stress responses such as phospho-C-Jun N-terminal kinase (JNK) and C/EBP homologous protein (CHOP). Treatment of obese diabetic db/db mice with DW1182v preserved islet integrity and thus increased insulin secretion and lowered blood glucose after glucose infusion. These results suggest that a small molecule protecting HG/PA-induced glucolipotoxicity to beta cells can be a new therapeutic candidate to prevent the development of type 2 diabetes.

Low-temperature (∼270 °C) growth of vertically aligned ZnO nanorods using photoinduced metal organic vapour phase epitaxy

Yatsui, T,Lim, J,Nakamata, T,Kitamura, K,Ohtsu, M,Yi, G-C IOP Pub 2007 Nanotechnology Vol.18 No.6

<P>We successfully produced a drastic decrease in the required growth temperature of single-crystalline ZnO nanorods, and enabled successful growth of vertically aligned ZnO nanorods on a Si(100) substrate using photoinduced metal organic vapour phase epitaxy (MOVPE). We introduced 325 nm light during the MOVPE growth, and achieved vertical growth of single-crystalline ZnO nanorods with a hexagonal crystal structure on Si(100) at a growth temperature of 270 °C. The successful low-temperature growth of ZnO nanorods on the Si(100) substrate described here is a promising step toward designing nanoscale photonic and electronic devices required by future systems.</P>

Novel signaling axis for ROS generation during K-Ras-induced cellular transformation

Park, M-T,Kim, M-J,Suh, Y,Kim, R-K,Kim, H,Lim, E-J,Yoo, K-C,Lee, G-H,Kim, Y-H,Hwang, S-G,Yi, J-M,Lee, S-J Macmillan Publishers Limited 2014 CELL DEATH AND DIFFERENTIATION Vol.21 No.8

Reactive oxygen species (ROS) are well known to be involved in oncogene-mediated cellular transformation. However, the regulatory mechanisms underlying ROS generation in oncogene-transformed cells are unclear. In the present study, we found that oncogenic K-Ras induces ROS generation through activation of NADPH oxidase 1 (NOX1), which is a critical regulator for the K-Ras-induced cellular transformation. NOX1 was activated by K-Ras-dependent translocation of p47<SUP>phox</SUP>, a subunit of NOX1 to plasma membrane. Of note, PKCδ, when it was activated by PDPK1, directly bound to the SH3-N domain of p47<SUP>phox</SUP> and catalyzed the phosphorylation on Ser348 and Ser473 residues of p47<SUP>phox</SUP> C-terminal in a K-Ras-dependent manner, finally leading to its membrane translocation. Notably, oncogenic K-Ras activated all MAPKs (JNK, ERK and p38); however, only p38 was involved in p47<SUP>phox</SUP>-NOX1-dependent ROS generation and consequent transformation. Importantly, K-Ras-induced activation of p38 led to an activation of PDPK1, which then signals through PKCδ, p47<SUP>phox</SUP> and NOX1. In agreement with the mechanism, inhibition of p38, PDPK1, PKCδ, p47<SUP>phox</SUP> or NOX1 effectively blocked K-Ras-induced ROS generation, anchorage-independent colony formation and tumor formation. Taken together, our findings demonstrated that oncogenic K-Ras activates the signaling cascade p38/PDPK1/PKCδ/p47<SUP>phox</SUP>/NOX1 for ROS generation and consequent malignant cellular transformation.

Investigation of lauric acid dopant as a novel carbon source in MgB₂ wire

Lee, C.M.,Lee, S.M.,Park, G.C.,Joo, J.,Lim, J.H.,Kang, W.N.,Yi, J.H.,Jun, B.H.,Kim, C.J. North-Holland 2010 Physica. C, Superconductivity Vol.470 No.20

We fabricated lauric acid (LA) doped MgB<SUB>2</SUB> wires and investigated the effects of the LA doping. For the fabrication of the LA-doped MgB<SUB>2</SUB> wires, B powder was mixed with LA at 0-5wt.% of the total amount of MgB<SUB>2</SUB> using an organic solvent, dried, and then the LA-treated B and Mg powders were mixed stoichiometrically. The powder mixture was loaded into an Fe tube and the assemblage was drawn and sintered at 900<SUP>o</SUP>C for 3h under an argon atmosphere. We observed that the LA doping induced the substitution of C for the B sites in MgB<SUB>2</SUB> and that the actual content of C increased monotonically with increasing LA doping level. The LA-doped MgB<SUB>2</SUB> wires exhibited a lower critical temperature (T<SUB>c</SUB>), but better critical current density (J<SUB>c</SUB>) behavior in a high magnetic field: the 5wt.% LA-doped sample had a J<SUB>c</SUB> value of 5.32x10<SUP>3</SUP>A/cm<SUP>2</SUP>, which was 2.17 times higher than that of the pristine sample (2.45x10<SUP>3</SUP>A/cm<SUP>2</SUP>) at 5K and 6T, suggesting that LA is an effective C dopant in MgB<SUB>2</SUB> for enhancing the high-field J<SUB>c</SUB> performance.

Effect of Implant Tilt Angle on the Electrical Properties of Source/Drain Junctions in Submicron MOSFET's

Yi, Y. G.,Kim, C. T.,Ko, C. G.,Chun, H. G.,Oh, K. H. 대한전자공학회 1991 ICVC : International Conference on VLSI and CAD Vol.2 No.1

An investigation is made on the influence of implant tilt angle on the electrical characteristics of shallow n+/p junctions. When the ions are implanted into Si through 20 nm thick oxide layers, no significant difference is observed in the ion distribution profiles of the junctions fabricated with non-tilted arid 7° tilted implants. For a dose of 1.0E13/㎠, lower sheet resistance is observed in the junction formed with non-tilted implantation. It is shown that n-channel MOSIETs fabricated by non-tilted source-drain implants result in enhanced symmetrical channel electric field as source and drain are interchanged.