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A Nickel Nanowire Diluter Operating through the Principle of the Dielectrophoretic Attraction Force
양진호(Jinho Yang),윤현중(Hyeun Joong Yoon),양의혁(Eui-Hyeok Yang),양상식(Sang Sik Yang) 대한전기학회 2010 전기학회논문지 Vol.59 No.2
This paper presents a microfabricated nanowire diluter which dilutes the concentration of nanowires in solution instead of by the conventional centrifuge process. The device has 16 pairs of gold electrodes in a micro channel composed of a glass substrate and PDMS. We prepared nickel nanowires by the template-directed electrodeposition method using nanoporous anodized aluminum template (AAO). We injected the Dimethylformamide (DMF) solution containing nanowires into the inlet of the diluter while applying square wave voltages on the electrodes to trap the nanowires at the subsequent gold electrodes by means of dielectrophoretic attraction forces. The concentration of nanowires at the outlet of the micro channel was changed as we expected, which illustrates that the device can effectively dilute nanowires and can be applied to a controlled assembly of nanowires.
Lee, Hyeok-Won,Park, Jung-Ho,Lee, Hee-Suk,Kim, Chun-Suk,Lee, Jin-Gyeom,Kim, Won-kyo,Ryu, Kyoung-Hwa,Ahn, Jung-Oh,Lee, Eun-Gyo,Kim, Seon-Won,Jeon, Jong-Min,Yang, Yung-Hun,Choi, Eui-Sung,Lee, Hong-Weon Society for Bioscience and Bioengineering, Japan 2019 Journal of bioscience and bioengineering Vol. No.
<P>Many volatile compounds, such as isoprene, a precursor used in the synthesis of natural rubber, have been produced through fermentation using genetically engineered microorganisms. Despite this biotechnological success, measuring the concentrations of volatile compounds during fermentation is difficult because of their high volatility. In current systems, off-line analytical methods usually lead to product loss, whereas on-line methods raise the production cost due to the requirement of complex devices. Here, we developed a novel on-line gas chromatography (GC)-based system for analyzing the concentration of isoprene with the aim to minimize the cost and requirement for devices as compared to current strategies. In this system, a programmable logic controller is used to combine conventional GC with a syringe pump module (SPM) directly connected to the exhaust pipe of the fermentor, and isoprene-containing samples are continuously pumped from the SPM into the GC using an air cylinder recycle stream. We showed that this novel system enables isoprene analysis during fermentation with convenient equipment and without the requirement of an expensive desorption tube. Furthermore, this system may be extended to the detection of other volatile organic compounds in fermentation or chemical processes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> This system analyzes the concentration of isoprene in aerobic fermentation. </LI> <LI> PLC is used to combine conventional GC with SPM directly connected to fermenter. </LI> <LI> Produced isoprene is continuously analyzed using the GC. </LI> <LI> This system may be extended to the detection of other volatile organic compounds. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>