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Monomer Composition of Pear and Persimmon Cutin
Kim, Yu Sam,Jang, Sei Heon,Espelie, Karl 생화학분자생물학회 1987 BMB Reports Vol.14 No.2
Monomer composition of pear and persimmon cutin was analyzed by a combined,; gas chromatography-mass spectrometry (GC-mass). Those cutins, which are surface biopolyesters covered the fruits, were isolated by treatment with ammonium oxalateoxalic acid and pectinase. The cutins, defatted by extraction, were depolymerized by metal hydride reduction. Ether extractable fraction of the reaction mixture w as silylated and analyzed by GC-mass. Persimmon cutin was composed of a relatively small number of monomers which is mainly w-hydoxy-9, 10-epoxyoctadecanoic acid (47.8%). Pear cutin was composed of a larger number of monomers which include long chain fatty alcohol, fatty acid and dicarboxylic acids indicating suberin characteristics. Relative amount of w-hydroxy-9, 10-epoxyoctadecanoic acid in pear cutin (76.1%) was considerably higher than that in persimmon cutin.