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- 저자 : Elsnoussi Ali Hussin Mohamed (검색결과 2 건)
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Elsnoussi Ali Hussin Mohamed,Mun Fei Yam,Lee Fung Ang,Ali Jimale Mohamed,Mohd. Zaini Asmawil 사단법인약침학회 2013 Journal of Acupuncture & Meridian Studies Vol.6 No.1
Orthosiphon stamineus is a popular folk medicine widely used to treat many diseases including diabetes. Previous studies have shown that the sub-fraction of chloroform extract was able to inhibit the rise of blood glucose levels in a glucose tolerance test. This study was carried out to evaluate the chronic effect and possible mechanism of action of the bioactive chloroform sub-fraction of O. stamineus using streptozotocin-induced diabetic rats and in vitro methods. Administration of the chloroform extract sub-fraction 2 (Cƒ2-b) at a dose of 1 g/kg twice daily on diabetic rats for 14 days showed a significant lowering (p < 0.05) of the final blood glucose level compared to the pretreatment level. However, there were no significant differences in the plasma insulin levels post-treatment compared to the pretreatment levels for all doses of Cƒ2-b. Conversely, Cƒ2-b at a concentration of 2 mg/mL significantly increased (p < 0.001) the glucose uptake by the rat diaphragm muscle. The increase in glucose uptake was also shown when the muscle was incubated in a solution containing 1 IU/mL of insulin or 1 mg/mL of metformin. Furthermore, the effect of this sub-fraction on glucose absorption in the everted rat jejunum showed that Cƒ2-b at concentrations of 0.5 mg/mL, 1 mg/mL and, 2 mg/mL significantly reduced the glucose absorption of the jejunum (p < 0.05–0.001). Similarly, the absorption of glucose was also inhibited by 1 mg/mL and 2 mg/mL of metformin (p < 0.001). These results suggest that the effect of Cƒ2-b may be due to extra-pancreatic mechanisms. There was no evidence that the plant extract stimulated the release of insulin in order to lower the blood glucose level.
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Mun Fei Yam,Elsnoussi Ali Hussin Mohamed,Lee Fung Ang,Li Pei,Yusrida Darwis,Roziahanim Mahmud,Mohd. Zaini Asmawi,Rusliza Basir,Mariam Ahmad,유혜현,김동현,정혜진 사단법인약침학회 2012 Journal of Acupuncture & Meridian Studies Vol.5 No.4
Orthosiphon stamineus extracts contain three flavonoids (3’-hydroxy-5, 6, 7, 4’-tetramethoxyflavone, sinensetin, and eupatorin) as bioactive substances. Previous reported high performance liquid chromatography- ultraviolet (HPLC-UV) methods for the determination of these flavonoids have several disadvantages, including unsatisfactory separation times and not being well validated according to International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) standard guidelines. A rapid, specific reversed-phase HPLC method with isocratic elution of acetonitrile: isopropyl alcohol: 20 mM phosphate buffer (NaH2-PO4) (30:15:55, v/v) (pH 3.5) at a flow-rate of 1 ml/minute, a column temperature of 25_C, and ultraviolet (UV) detection at 340 nm was developed. The method was validated and applied for quantification of different types of O stamineus extracts and fractions. The method allowed simultaneous determination of 3’-hydroxy-5,6,7,4’- tetramethoxyflavone, sinensetin, and eupatorin in the concentration range of 0.03052 e250 mg/ml. The limits of detection and quantification, respectively, were 0.0076 and 0.061 mg/ml for 3’-hydroxy-5,6,7,4’-tetramethoxyflavone, 0.0153 and 0.122 mg/ml for sinensetin and 0.0305 and 0.122 mg/ml for eupatorin. The percent relative standard deviation (% RSD) values for intraday were 0.048e0.368, 0.025e0.135, and 0.05e0.476 for 3’-hydroxy-5,6,7,4’-tetramethoxyflavone, sinensetin, and eupatorin, respectively, and those for intraday precision were 0.333e1.688, 0.722e1.055, and 0.548e1.819, respectively. The accuracy for intraday were 91.25%e103.38%, 94.32%e109.56%, and 92.85%e109.70% for 30-hydroxy-5,6,7,40-tetramethoxyflavone, sinensetin, and eupatorin, respectively, and those for interday accuracy were 97.49%e103.92%, 103.58% e104.57%, and 103.9%e105.33%, respectively. The method was found to be simple, accurate and precise and is recommended for routine quality control analysis of O stamineus extract containing the three flavonoids as the principle components in the extract.
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