http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
김창민(Chang Min Kim),Y. Ebizuka(Y. Ebizuka),U. Sankawa(U. Sankawa) 한국생약학회 1987 생약학회지 Vol.18 No.1
A. further investigation of the flavonoid constituents of the underground parts of Echinosophora koreensis Nakai(Leguminosae) has yielded a new isoflavonoid, mp 194∼7˚, together with the known dihydroflavonol, sophoronol, mp 104∼8˚, The new isoflavonoid was identified as 3, 5, 7-trihydroxy-2`, 4`-dimethoxy - 3` - isopentenylisoflavanone on the basis of spectroscopic means. The latter previously reported as 2[(5`-methoxy-2`, 2`-dimethyl-2H-benzopyran)6`-yl]-3, 5, 7-trihydroxychroman- 4 -one should be revised to 3-[(5`-methoxy-2`, 2`-dimethyl-2H-benzopyran)- 6` -yl)-3, 5, 7-trihydroxychroman-4-one by spectral data.
김창민(Chang Min Kim),U. Sankawa(U. Sankawa),Y. Ebizuka(Y. Ebizuka) 한국생약학회 1987 생약학회지 Vol.18 No.1
A new isoflavanone, echinoisosophoranone mp 185∼7˚ has been isolated from the underground parts of Echinosophora koreensis Nakai (Leguminosae). The structure of echinoisosophoranone was identified as 5, 4`-dihydroxy-5`-(α, α-dimethylallyl)-7, 2`-dimethoxy isoflavanone on the basis of spectroscopic studies.
MOLECULAR CLONING OF cDNA ENCODING HUMAN LANOSTEROL SYNTHASE
Sankawa, Ushio,Sung, Chung Ki,Shibuya, Masaaki,Ebizuka, Yutaka 전남대학교 약품개발연구소 1996 약품개발연구지 Vol.4 No.1
A cDNA encoding human lanosterol synthase, the enzyme responsible for the backbone formation step in sterol biosynthesis, was cloned by extensive application of PCRs. Five degenerate oligonucleotide primers(139S, 440S, 528A, 575A and 712A) corresponding to the homologous amino acid sequences among the known 2,3-oxidosqualene cyclase(OSC) were designed. PCR with one pair(440S and 528A) of five primers yielded a 285-by fragment. PCRs with the primers based on the obtained fragment and the degenerate primers (139S and 712A) gave longer fragments. Finally, full nucleotide sequence of cDNA was obtained by a $quot;rapid amplification of cDNA ends$quot; (RACE) method.
p-Coumaroylamino Acids from Yeast-Elicited Ephedra distachya Cultures
Song, Kyung-Sik,Sankawa, Ushio,Ebizuka, Yutaka The Pharmaceutical Society of Korea 1994 Archives of Pharmacal Research Vol.17 No.1
Three p-coumaroylamino acids (p-CAAs) were isolated from the yeast-elicited Ephedra distachya cultures by consecutive purification using XAD_2, silicagel and RP-HPLC. Retention times on HPLC as well as their UV, IR, NMR and MS spectral data indicated that the yeast-induced p-CAAs wre p-coumaroyl--D-valine, p-coumaroyl-D-serine and p-coumarouyl-D-threonine, respectively. The structures of p-CAAs were confirmed by the comparison of their physico-chemical properties 3with those of synthetic ones. They were isolated and identified for the first time from natural products and supposed to be accumulated as phytoalexins of Ephedra.
Elicitors which Induce the Accumulation of p-Coumaroylamino Acids in Ephedra distachya Cultures
Song, Kyung-Sik,Sankawa, Ushio,Ebizuka, Yutaka The Pharmaceutical Society of Korea 1994 Archives of Pharmacal Research Vol.17 No.1
Some ammonium oxalate soluble pectic fragments prepared from cultured cell wall of Ephycla distrahya elicited the accumulation of p-coumarocylamino acids (p-CAA) in E. distachya cultures while water soluble and alkali soluble fractions had no activity. Partial purification of the pectic fragments fraction using DEAE-cellulose chromatography afforded two active fractions (PS-I and PS-II) which were composed of mainly uronic acids (98-99 w/w %). They elicited the accumulation of p-CAA in an amount of 52-60 nmol per gram fresh weight of cultures. The acidic sugar compositions of PS-I and PS-II were found to be galacturonic acid and glucuronic acid by TLC analysis. They were supposed to act as endogenous elicitors of p-CAA accumulation. In order to investigate the effect of ethylene on p-CAA accumulation, Ethrel, which is known as ethylene generator, and ACC(1-aminocyclopropane-1-carboxylic acid), a direct precusor of ethylene biosynthesis, were added to the culture. However, they did not glycopeptide elicitor [(Con A-II)], either. Consequently, no relationships between ethylene and p-CAA accumulation were recognized. Several tentative elicitors were teted for their activity. Commercial yeast glucan, $CuCl_2$, laminarin and laminariheptaose had slight activity whereas ${\alpha}$-methylmannopyranoside and commercial yeast mannan had no elicitor activity. ${\alpha}$-methylmannopyranoside which has been known as a tentative inhibitor of glucan elicitor in Glycine max did not affect on the elicitor activity of Con A-II.
A convinient method to evaluate promoter activity with transformed hairy root culture
Jong Soo Lee,Osamu Nakajima,Masaaki Shibuya,Yutaka Ebizuka,Ushio Sankawa 전남대학교 약품개발연구소 1995 약품개발연구지 Vol.3 No.1
Transformed hairy roots of tobacco were used to measure activity of some promoters, horseradish peroxidase promoter (POP), 500 bp and 300 bp region of 5' upstream from Pueraria lobata chalcone synthase gene (CHSpro500, CHSpro300) and cauliflower mosaic virus 35S (CaMV 35S) promoter, which were fused to β-glucuronidase (GUS) gene, respectively. Hairy roots induced by Agrobacterium rhizogenes exhibited GUS activity in strength order of POP, CaMV 35S, CHSpro500 and CHSpro300, and it was suggested that for introduction and expression of foreign gene into the plant genome, POP may be applied as the potent promoter of plant origin in place of CaMV 35S promoter. Moreover, elicitor-response of Pueraria lobata CHS promoter was investigated with this assay system and it was verified that CHS promoter responds well to exogenous elicitor, yeast extract and CaCl₂. Although protoplasts or transgenic plants have been used widely as the materials for that purpose for the last decade, our results suggested that our alternative method was the simple and reasonable one to evaluate promoter activity.