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Ma, Gang,Marzec, Nancy,Dziak, Rosemary Korean Academy of Oral Biology and the UCLA Dental 2001 International Journal of Oral Biology Vol.26 No.1
The goal of this present study was to investigate the effects of insulin-like growth factor (IGF-I) on osteoblastic cell proliferation, and the possible involvement of the alpha 1 subunit of L-type voltage activated calcium channels (L-VACC) in the regulation of these effects. In rat calvarial osteoblastic cells, incubation with 100 nM IGF-I for 24 hours significantly increased cell proliferation. The IGF-I-induced increase in cell proliferation was inhibited by verapamil (10-100 μM), a blocker of L-VACCs. In order to further investigate which isoform(s) of L-VACCs is involved in the effect of IGF-I, antisense oligodeoxynucleotides (ODNs) specific for alpha 1S (skeletal), alpha 1C (cardiac) or alpha 1D (neuroendocrine) of L-VACC were used to inhibit the expression of the different isoforms. The results showed that the proliferative response to IGF-I was significantly inhibited by the antisense ODN directed to the alpha 1C of L-VACC (decrease by 28%, P<0.05, ANOVA), but not by sense to alpha 1C or antisense/sense ODNs to alpha 1S and alpha 1D. The data suggest that the cardiac isoform of the L-VACC is at least partially responsible for IGF-I-induced increases in rat osteoblastic cell proliferation.
Effects of 1,25 Dihydroxyvitamin D_3 on PKC Isoform Expression in Osteoblastic Cells
Lampasso, J.D.,Intini, Guiseppe,Dziak, R. Korean Academy of Oral Biology and the UCLA Dental 2000 International Journal of Oral Biology Vol.25 No.3
Eleven isoforms encoded by different genes have been identified in the protein kinase C〔PKC〕family of homologous serine/threonine protein kinases. These PKC isoforms are major intracellular mediators that control cell proliferation and differentiation in a variety of cell systems. The expression and function of the different isoforms are tissue specific and mediate specific physiological processes. To date, information on the PKC isoforms expressed in normal rat osteoblasts as well as their specific pattern of expression is limited. With the use of Western blotting technique we have identified which of the eleven PKC isoforms as a function of time in culture. Our data indicate that PKC α, γ, ι, ε, λ, ζ, μ and δ are all expressed in normal rat osteoblastic cells, and that these follow a specific pattern of expression. The expression of α, γ and ι isoforms correlated with increasing time of cells in culture, while the isoforms ζ, μ and δ displayed a decrease in expression in more mature cell cultures. Furthermore, 1.25 Dihydroxyvitamin D_3 differentially regulated the expression of specific isoforms. These studies have provided us with original information on the types of PKC isoforms present in normal rat osteoblastic cells and further insight into their possible role in osteoblastic cell maturation.