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RISS 인기검색어
- 검색키워드
- 저자 : Dourado, Grace KZS (검색결과 2 건)
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Chemopreventive Actions of Blond and Red-Fleshed Sweet Orange Juice on the Loucy Leukemia Cell Line
Dourado, Grace KZS,Stanilka, Joy M,Percival, Susan S,Cesar, Thais B Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.15
Background: Red-fleshed sweet orange juice (ROJ) comes from a new variety of citrus cultivated in Brazil that contains high levels of ${\beta}$-carotene and lycopene, and similar amounts of hesperidin (HSP) and nutrients, equivalently to blond orange juice (BOJ). Such bioactive compounds are associated with chemopreventive actions in several cancer cell lines. The purpose of this study was to examine the cytotoxicity, cell cycle, apoptosis, and cytokine secretion after BOJ, ROJ, and HSP treatment of a novel T acute lymphoblastic leukemia cell line, Loucy. Materials and Methods: Loucy cells were incubated for 24-h with BOJ, ROJ, and HSP, and the viability was measured using trypan blue. Cell cycling and apoptosis were assessed by propidium iodide (PI) and annexin V-FITC/PI flow cytometry, respectively. Secretion of cytokines $IL-1{\alpha}$, $IL1-{\beta}$, IL-2, IL-4, IL-6, IL-10, IL-17A, $IFN{\gamma}$, $TNF{\alpha}$, $TGF{\beta}$, $MIP{\alpha}$, and $MIP{\beta}$ was determined by ELISA array. Results: BOJ and ROJ treatments promoted Loucy cell cytotoxicity. Additionally, BOJ induced cell cycle arrest in the G0/G1 phase, and decreased the cell accumulation in the G2/M. ROJ decreased only the G0/G1 fraction, while HSP did not change the cell cycle. BOJ led to apoptosis in a different fashion of ROJ, while the first treatment induced apoptosis by increase of late apoptosis and primary necrotic fractions, the second increased early and late apoptosis, and primary necrotic fraction compared to positive controls. HSP had no effect on apoptosis. IL-6 and IL-10 were abrogated by all treatments. Conclusions: Taking together, these results suggest potential chemopreventive effects of BOJ and ROJ on Loucy cells.
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Physiological and yield responses of soybean under water deficit
Felisberto Guilherme,Schwerz Felipe,Umburanas Renan Caldas,Dourado-Neto Durval,Reichardt Klaus 한국작물학회 2023 Journal of crop science and biotechnology Vol.26 No.1
Water defcit during grain flling is a key factor on soybean (Glycine max (L.) Merrill) production, but plant response to diferent water stress levels should be better understood. This study evaluated soybean plant response to diferent soil water levels during grain flling. It was assessed the gravimetrical humidity and soil matric potential, leaf relative water content, leaf water potential, proline, and yield components of plants under a range of days without irrigation (0, 3, 6, 9, and 12) during grain flling phase. Until soil matric potential was around −0.8 atm soybean water defcit tolerance mechanisms were enough to maintain leaf relative water content and leaf water potential at acceptable levels, which proline concentration was a key factor in this mechanism. Leaf relative water content and leaf water potential showed to be related in maintaining soybean yield under water defcit and they may be used in studies of soybean tolerant cultivars to water restriction. From 9 days on, critical yield losses were observed due to water stress. The information presented in this study supports soybean producers in decision-making in irrigated systems to minimize productivity losses due to water defcit during the critical period of the crop–grain flling.
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