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Development of a universal plate-agglutination test for detecting Haemophilus parasuis
Dingqian Guo,Quan Hai,Guoqing Shao,Hua Yue 대한수의학회 2010 JOURNAL OF VETERINARY SCIENCE Vol.11 No.4
Due to the serovar diversity in Haemophilus (H.) parasuis, it is difficult to develop a universal serological method for detection of this pathogen. Here, we report a universal plate-agglutination test for detecting H. parasuis. Diagnostic antisera were prepared by mixing antisera of serovars 4, 5, 12, 13 and 14 in the optimized ratio. The results of the plate-agglutination test showed that the diagnostic antisera could agglutinate with all 15 reference strains of H. parasuis and 74/75 clinical isolates. Further, the specificity of the method was validated with 22 bacterial strains from 12 related species.