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        Wax glands ultrastructure and chemical composition of wax of giant mealybug Drosicha stebbingii (Green) (Hemiptera: Monophlebidae)

        Ahmad Ayashaa,Kundu Aditi,Dey Debjani 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.2

        Droschia stebbingii (Green) belonging to the family Monophlebidae (Hemiptera: Coccoidea) is a serious, phytophagous and dimorphic pest. D. stebbingii and D. mangiferae (Green) with sixty-two common hosts and minor morphological variations pose problems towards their taxonomic identification. Light and scanning electron microscopy were used to outline the ultrastructural details of cuticular wax producing structures of D. stebbingii. Three types of wax-pores along with tubular duct were observed on the head, thorax and abdomen. Quinquelocular and multilocular disc-pores exhibited variations in the central loculus with single and double opening. Short curly wax strands were secreted from the outer loculi whereas central loculus secreted long tubular strands. Additionally, bilocular pores secreting curly wax were observed having two large loculi separated by a septum. GC–MS analysis of cuticular wax indicated the presence of hydrocarbons and wax esters forming majority of the covering along with long chain alcohols, ketones and acids. Variations in the percentage of hydrocarbon and wax esters content were observed for two populations of D. stebbingii collected from different hosts. Hydrocarbons were abundant in populations collected on Pongamia pinnata (L.) Pierre whereas long chain esters dominated the wax profile for the population collected from Tectona grandis L.f. Compositional variations in the cuticular wax of D. stebbingii on different host along with knowledge on the ultrastructural wax pores and waxy secretion would provide an insight to the existing knowledge of this scale insect.

      • Green synthesis of Nerium oleander-conjugated gold nanoparticles and study of its in vitro anticancer activity on MCF-7 cell lines and catalytic activity

        Barai Abir Chandan,Paul Koushik,Dey Aditi,Manna Subhankar,Roy Somenath,Bag Braja Gopal,Mukhopadhyay Chiradeep 나노기술연구협의회 2018 Nano Convergence Vol.5 No.10

        The phytochemicals present in the stem bark extract of Nerium oleander (commonly known as Karabi) have been utilized for the green synthesis of stable gold-conjugated nanoparticles at room temperature under very mild conditions. The green synthesized gold-conjugated nanoparticles were characterized by surface plasmon resonance spectroscopy, High resolution transmission electron microscopy, X-ray diffraction studies and dynamic light scattering. A mechanism for the synthesis and stabilization of gold-conjugated nanoparticles (AuNPs) has been proposed. Anticancer activity of the stabilized AuNPs studied against MCF-7 breast cancer cell line revealed that the stabilized AuNPs were highly effective for the apoptosis of cancer cells selectively. The antioxidant activity of the stem bark extract of Nerium oleander has also been studied against a long lived 2,2-diphenylpicrylhydrazyl radical at room temperature. Moreover, the utilization of the stabilized AuNPs as a catalyst has also been demonstrated.

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        Airway Management for Initial PEG Insertion in the Pediatric Endoscopy Unit: A Retrospective Evaluation of 168 Patients

        Peck, Jacquelin,Nguyen, Anh Thy H.,Dey, Aditi,Amankwah, Ernest K.,Rehman, Mohamed,Wilsey, Michael The Korean Society of Pediatric Gastroenterology 2021 Pediatric gastroenterology, hepatology & nutrition Vol.24 No.1

        Purpose: Percutaneous endoscopic gastrostomy (PEG) tube placements are commonly performed pediatric endoscopic procedures. Because of underlying disease, these patients are at increased risk for airway-related complications. This study compares patient characteristics and complications following initial PEG insertion with general endotracheal anesthesia (GETA) vs. anesthesia-directed deep sedation with a natural airway (ADDS). Methods: All patients 6 months to 18 years undergoing initial PEG insertion within the endoscopy suite were considered for inclusion in this retrospective cohort study. Selection of GETA vs. ADDS was made by the anesthesia attending after discussion with the gastroenterologist. Results: This study included 168 patients (GETA n=38, ADDS n=130). Cohorts had similar characteristics with respect to sex, race, and weight. Compared to ADDS, GETA patients were younger (1.5 years vs. 2.9 years, p=0.04), had higher rates of severe American Society of Anesthesiologists (ASA) disease severity scores (ASA 4-5) (21% vs. 3%, p<0.001), and higher rates of cardiac comorbidities (39.5% vs. 18.5%, p=0.02). Significant associations were not observed between GETA/ADDS status and airway support, 30-day readmission, fever, or pain medication in unadjusted or adjusted models. GETA patients had significantly increased length of stay (eβ=1.55, 95% confidence interval [CI]=1.11-2.18) after adjusting for ASA class, room time, anesthesia time, fever, and cardiac diagnosis. GETA patients also had increased room time (eβ=1.20, 95% CI=1.08-1.33) and anesthesia time (eβ=1.50, 95% CI=1.30-1.74) in adjusted models. Conclusion: Study results indicate that younger and higher risk patients are more likely to undergo GETA. Children selected for GETA experienced longer room times, anesthesia times, and hospital length of stay.

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