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      • KCI등재

        bacterial and Antifungal Properties of Acetonic Extract of Feijoa sellowiana Fruits and Its Effect on Helicobacter pylori Growth

        Adriana Basile,Barbara Conte,Daniela Rigano,Felice Senatore,Sergio Sorbo 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.1

        Feijoa sellowiana Berg. fruit, widely used for human consumption, is well appreciated for its good nutritional characteristics and for its pleasant flavor and aroma. In a prior study we showed that the acetonic extract of F. sellowiana fruit exerts a potent antibacterial activity against some Gram-positive and Gram-negative bacterial strains. To confirm and deepen the study on antimicrobial activities of the fruit, in this article we analyze the same extract for its effectiveness against different kind of bacteria both as American Type Culture Collection standard strains and clinically isolates, the Gram-negative Helicobacter pylori, and three fungal strains (one human pathogen and two phytopathogens). Our aim was also to isolate and define the active component(s) involved in the antibacterial, antifungal, and anti-H. pylori activity of the extract through activity-guided fractionation procedures. This allowed us to identify flavone as the active compound of F. sellowiana fruit. Flavone showed a high antibacterial activity against the nine standard bacterial strains tested and the matched clinically isolated bacterial strains and was significantly more active against H. pylori than metronidazole. Pseudomonas aeruginosa, Proteus mirabilis, and Proteus vulgaris from both standard and clinical sources showed a very high sensitivity to flavone (minimal inhibitory concentration [MIC]=1.95μg/mL for standard strains and 3.9μg/mL for clinical isolates of the three bacteria), showing also good minimal bactericidal concentration values. Among fungi tested, Rhyzoctonia solani was the most sensitive strain to the action of flavone (MIC=62.5μg/mL).

      • KCI등재

        Essential Oil Composition of Teucrium divaricatum Sieb. ssp. villosum (Celak.) Rech. fil. Growing Wild in Lebanon

        Carmen Formisano,Francesco Napolitano,Daniela Rigano,Nelly Apostolides Arnold,Franco Piozzi,Felice Senatore 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.5

        Teucrium divaricatum Sieb. ssp. villosum (Celak.) Rech. fil. is a medicinal plant used in Lebanon. The hydrodistillation of the aerial parts of T. divaricatum yielded 0.27% (wt/wt) of essential oil; gas chromatography and gas chromatography/mass spectrometry analysis enabled the identification of 60 compounds representing 94.5% of the total oil. On the whole, the oil was constituted mainly by sesquiterpenes (64.6%), particularly sesquiterpene hydrocarbons (50.0%). The most abundant compounds were (E)-caryophyllene (30.1%) and caryophyllene oxide (6.1%). The in vitro activity of the essential oil against some microorganisms in comparison with gentamicin by the broth dilution method was determined. The oil exhibited a good activity as an inhibitor of growth of Gram-positive bacteria.

      • KCI등재

        Constituents of Leaves and Flowers Essential Oils of Helichrysum pallasii (Spreng.) Ledeb. Growing Wild in Lebanon

        Carmen Formisano,Enrico Mignola,Daniela Rigano,Felice Senatore,Nelly Apostolides Arnold,Maurizio Bruno,Sergio Rosselli 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.1

        The chemical compositions of the essential oils obtained from leaves and flowers of Helichrysum pallasii were analyzed by gas chromatography and gas chromatography-mass spectrometry. Among the 102 identified constituents, hexadecanoic acid (16.2%), (Z,Z)-9,12-octadecadienoic acid (6.8%), tetradecanoic acid (2.6%), and (Z)-caryophyllene (4.2%) were the main constituent of the oil from leaves, while in the oil from flowers hexadecanoic acid (14.7%), (Z,Z)-9,12-octadecadienoic acid (14.2%), (Z)-caryophyllene (3.6%), and δ-cadinene (3.1%) predominated. The oils were both characterized by sesquiterpenes (33.4% for leaves and 33.7% for flowers, respectively) and fatty acids and esters (30.3% in leaves and 35% in flowers, respectively). The in vitro activity of the essential oils of the plant against some microorganisms in comparison with chloramphenicol by the broth dilution method was determined. The oils exhibited a weak activity as inhibitors of growth of Staphylococcus epidermidis in vitro (minimum inhibitory concentration = 100 μg/mL).

      • KCI등재

        Constituents of Leaves and Flowers Essential Oils of Helichrysum pallasii (Spreng.) Ledeb. Growing Wild in Lebanon

        Formisano, Carmen,Mignola, Enrico,Rigano, Daniela,Senatore, Felice,Arnold, Nelly Apostolides,Bruno, Maurizio,Rosselli, Sergio The Korean Society of Food Science and Nutrition 2009 Journal of medicinal food Vol.12 No.1

        The chemical compositions of the essential oils obtained from leaves and flowers of Helichrysum pallasii were analyzed by gas chromatography and gas chromatography-mass spectrometry. Among the 102 identified constituents, hexadecanoic acid (16.2%), (Z,Z)-9,12-octadecadienoic acid (6.8%), tetradecanoic acid (2.6%), and (Z)-caryophyllene (4.2%) were the main constituent of the oil from leaves, while in the oil from flowers hexadecanoic acid (14.7%), (Z,Z)-9,12-octadecadienoic acid (14.2%), (Z)-caryophyllene (3.6%), and $\delta$-cadinene (3.1%) predominated. The oils were both characterized by sesquiterpenes (33.4% for leaves and 33.7% for flowers, respectively) and fatty acids and esters (30.3% in leaves and 35% in flowers, respectively). The in vitro activity of the essential oils of the plant against some microorganisms in comparison with chloramphenicol by the broth dilution method was determined. The oils exhibited a weak activity as inhibitors of growth of Staphylococcus epidermidis in vitro (minimum inhibitory concentration = $100\;{\mu}g/mL$).

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