http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Daniel Seitz (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
-
Andre´ Luiz A. Pizzolatti,Florian Gaudig,Daniel Seitz,Carlos R. M. Roesler,Gean Vitor Salmoria 한국조직공학과 재생의학회 2018 조직공학과 재생의학 Vol.15 No.6
BACKGROUND: Glucosamine hydrochloride (GlcNHCl) has been shown to inhibit cell growth and matrix synthesis, but not with N-acetyl-glucosamine (GlcNAc) supplementation. This effect might be related to an inhibition of critical growth factors (GF), or to a different metabolization of the two glucosamine derivatives. The aim of the present study was to evaluate the synergy between GlcNHCl, GlcNAc, and GF on proliferation and cartilage matrix synthesis. METHOD: Bovine chondrocytes were cultivated in monolayers for 48 h and in three-dimensional (3D) chitosan scaffolds for 30 days in perfusion bioreactors. Serum-free (SF) medium was supplemented with either growth factors (GF) TGF-b (5 ng mL-1) and IGF-I (10 ng mL-1), GlcNHCl or GlcNAc at 1mM each or both. Six groups were compared according to medium supplementation: (a) SF control; (b) SF ? GlcNHCl; (c) SF ? GlcNAc; (d) SF ? GF; (e) SF ? GF ? GlcNHCl; and (f) SF ? GF ? GlcNAc. Cell proliferation, proteoglycan, collagen I (COL1), and collagen II (COL2) synthesis were evaluated. RESULTS: The two glucosamines showed opposite effects in monolayer culture: GlcNHCl significantly reduced proliferation and GlcNAc significantly augmented cellular metabolism. In the 30 days 3D culture, the GlcNHCl added to GF stimulated cell proliferation more than when compared to GF only, but the proteoglycan synthesis was smaller than GF. However, GlcNAc added to GF improved the cell proliferation and proteoglycan synthesis more than when compared to GF and GF/GlcNHCl. The synthesis of COL1 and COL2 was observed in all groups containing GF. CONCLUSION: GlcNHCl and GlcNAc increased cell growth and stimulated COL2 synthesis in long-time 3D culture. However, only GlcNAc added to GF improved proteoglycan synthesis.
-
Finner, Kyle,Jee, M. James,Golovich, Nathan,Wittman, David,Dawson, William,Gruen, Daniel,Koekemoer, Anton M.,Lemaux, Brian C.,Seitz, Stella American Astronomical Society 2017 The Astrophysical journal Vol.851 No.1
<P>The second most significant detection of the Planck Sunyaev-Zel'dovich survey, PLCK G287.0+32.9 (z = 0.385), boasts two similarly bright radio relics and a radio halo. One radio relic is located similar to 400 kpc NW of the X-ray peak and the other similar to 2.8 Mpc to the SE. This large difference suggests that a complex merging scenario is required. A key missing puzzle for the merging scenario reconstruction is the underlying dark matter distribution in high resolution. We present a joint Subaru Telescope and Hubble Space Telescope weak-lensing analysis of the cluster. Our analysis shows that the mass distribution features four significant substructures. Of the substructures, a primary cluster of mass M-200c = 1.59(-0.22)(+0.25) x 10(15) h(70)(-1) M-circle dot dominates the weak-lensing signal. This cluster is likely to be undergoing a merger with one (or more) subcluster whose mass is approximately a factor of 10 lower. One candidate is the subcluster of mass M-200c = 1.16(-0.13)(+0.15) x 10(14) h(70)(-1) M-circle dot located similar to 400 kpc to the SE. The location of this subcluster suggests that its interaction with the primary cluster could be the source of the NW radio relic. Another subcluster is detected similar to 2 Mpc to the SE of the X-ray peak with mass M-200c =1.68(-0.20)(+0.22) x 10(14) h(70)(-1) M-circle dot. This SE subcluster is in the vicinity of the SE radio relic and may have created the SE radio relic during a past merger with the primary cluster. The fourth subcluster, M-200c = 1.87(-0.22)(+0.24) x 10(14) h(70)(-1) M-circle dot, is NW of the X-ray peak and beyond the NW radio relic.</P>
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
