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Marcio Crescencio,Alexandre Augusto Biz,Jose Leomar Todesco Smart Tourism Research Center 2024 Journal of smart tourism Vol.4 No.1
The objective of this article is to create a model of integrated management from the framework modeling of a digital collaborative network supported by knowledge engineering to make heritage site in the Brazil more effective. It is an exploratory and qualitative research with thematic analysis as technique of data analysis from the collaborative network, digital platform, world heritage, and tourism themes. The snowballing approach was chosen, and the mapping and classification of relevant studies was developed with the use of the spreadsheet tool and the Mendeley® software. The results show that the collaborative network model oriented towards strategic objectives should be supported by a digital platform that provides a technological environment that adds functionalities and digital platform services with the integration of knowledge engineering techniques and tools, enabling the discovery and sharing of knowledge in the collaborative network.
Pablo Emilio Vanegas-Espinoza,Crescencio Bazaldúa,Leticia Betsaida Ríos-Salomé,Octavio Paredes-López,Gustavo Adolfo Camelo-Méndez,Alma Angélica Del Villar-Martínez 한국원예학회 2020 Horticulture, Environment, and Biotechnology Vol.61 No.2
Plant tissue culture allows the maintenance of species of interest under controlled conditions. There have been few studiesrelated to the morphological changes that take place during callus formation and rediff erentiation processes. The aim ofthis work was to analyze the morphological changes during dediff erentiation and rediff erentiation processes in leaf explantsof marigold ( Tagetes erecta L.) using transmission electron microscopy, digital image analysis and concepts of fractaldimension. Leaf explants were sown in MS medium supplemented with 2.0 mg L −1 of benzyladenine and 2.0 mg L −1 of2,4-dichlorophenoxyacetic acid to induce dediff erentiation (callus formation). The rediff erentiation process (bud formation)was obtained with explants sown in MS medium supplemented with 3.0 mg L −1 indoleacetic acid and 0.5 mg L −1 benzyladenine. During the fi rst 8 days, the dediff erentiation process showed gradual changes in terms of the area and perimeter ofthe explants. The fractal dimension of the perimeter corresponding to the projected image of the calli changed from smoothto rough at day 20. These features indicate variations in tissue morphometry as a result of the callus formation process andfurther formation of cellular aggregates. Structural observations during the dediff erentiation process showed changes inshape and cellular organization as callus formation became evident. Round-shaped cells were observed in the callus, withfew organelles and a low degree of diff erentiation. Indirect organogenesis was achieved over a period of 10 days; in addition,plantlets developed from the obtained buds in 20 days in growth regulator-free MS medium.
Micropropagation of Aristolochia elegans(Mast.)
Lidia, Osuna T.,Alejandro, Mora I.,Elsa, Ventura Z.,Enrique, Jimenez F.,Crescencio, Bazaldua M.,Antonio, Jimenez A. The Korean Society of Crop Science 2007 Journal of crop science and biotechnology Vol.10 No.3
The roots of Aristolochia elegans Mast.(Aristolochiaceae) are widely used in Mexican traditional medicine as a remedy for scorpion venom. Current experimental evidence supports its purported antidote properties. However, collection from the wilderness has lead to local extinction of natural populations. In order to contribute to species preservation, cultivation, and standardization of morphological and pharmacological properties, a micropropagation method was developed. This includes in-vitro germination of seeds to produce aseptic plantlets, induction of multiple budding, and acclimatization. The treatment with benzylamino purine(10 ${\mu}M$) induced the highest number of buds(3.1 on average) in both types of explants. On the other hand, indolebutyric acid(1.5 ${\mu}M$) caused the highest root index(11.8) per explant. One hundred percent of the micropropagated plantlets developed vigorously after the acclimatization process.