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Chunqiu Guo,Long-Jin Wang,Fang Deng 제어·로봇·시스템학회 2020 International Journal of Control, Automation, and Vol.18 No.3
This paper considers the parameter identification for a class of nonlinear stochastic systems with colored noise. An input-output representation is derived by eliminating the state variables in the bilinear system. Based on the obtained identification model, a recursive generalized extend least squares algorithm is proposed by using the auxiliary model identification idea. Moreover, a two-stage recursive generalized extended least squares algorithm is presented to reduce the computational burden by using the hierarchical identification principle and the auxiliary model identification idea, respectively. A stochastic gradient identification algorithm is proposed for comparison. The simulation results show that the proposed algorithms have a good performance in estimating the parameters of the bilinear systems with colored noises.
Mast Cells Tryptase Promotes Intestinal Fibrosis in Natural Decellularized Intestinal Scaffolds
Wan Jian,Wu Tianqi,Liu Ying,Yang Muqing,Fichna Jakub,Guo Yibing,Yin Lu,Chen Chunqiu 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.4
BACKGROUND: Standard two-dimensional (2D) culture has confirmed the mechanism of mast cells (MCs) in the pathogenesis of inflammatory bowel disease (IBD), but the regulation of signaling responses of MCs may well differ in three-dimensional (3D) microenvironments. The aim of the study was to develop a 3D culture model based on decellularized intestinal scaffolds (DIS) and verify how MCs influenced fibroblasts phenotype in the 3D model. METHODS: DIS were achieved using the detergent technique and extracellular matrix (ECM) components were verified by histologic analysis, quantification and scanning electron microscope. After human colon fibroblasts recellularized into the scaffolds and activated by MCs tryptase and TGFb1, the changes in genes and signaling pathways during fibroblasts activation in 3D were studied and compared with the changes in 2D cell culture on plastic plates. RESULTS: Decellularization process effectively removed native cell debris while retaining natural ECM components and structure. The engrafted fibroblasts could penetrate into the scaffolds and maintain its phenotype. No matter whether fibroblasts were cultured in 2D or 3D, MCs tryptase and transforming growth factor b1 (TGF-b1) could promote the differentiation of fibroblasts into fibrotic-phenotype myofibroblasts through Akt and Smad2/3 signaling pathways. Furthermore, the pro-collagen1a1 and fibronectin synthesis of myofibroblasts in 3D was higher than in 2D culture. CONCLUSION: Our results demonstrated that the DIS can be used as a bioactive microenvironment for the study of intestinal fibrosis, providing an innovative platform for future intestinal disease modeling and screening of genes and signaling pathways.