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Kedong Song,Liying Li,Lili Ji,Siyuan Li,Chunfeng Mu,Yiwei Wang,Tianqing Liu 한국통합생물학회 2016 Animal cells and systems Vol.20 No.5
Fabrication and transplantation of tissue-engineered bones in a rotating wall vessel bioreactor (RWVB) was studied in the present study aiming to repair segmental bone defects. Osteoblasts were transfected with green fluorescent protein prior to seeding on bio-derived porous bone scaffolds at a density of 1 × 106 cells/mL and cultured in an RWVB for one week. For comparison, constructs were also cultured in a static condition. Morphology and structure of fabricated bones were examined using an inverted microscope, scanning electron microscope and histology analysis via hematoxylin–eosin and toluidine blue staining. Moreover, an animal model for repairing segmental bone defects of a Zelanian rabbit was used to assess the efficacy and biosafety of fabricated bones. In conclusion, tissue-engineered bones grew favorably in RWVB. In animal study, a preliminary repair of bone defects was noticed only in the experimental group after 4 weeks of implantation. Using RWVB, the fabricated tissue-engineered bone constructs were approved with better bio-capability in repairing the segmental bone defect.
Zhenhua Jiang,Xiujie Li,Shuling Zhang,Fugui Zhou,Jinhui Pang,Chunfeng Zhang,Dong Sun,Jianxin Mu,Guibin Wang 한국고분자학회 2011 Macromolecular Research Vol.19 No.5
Hyperbranched poly(ether ether ketone)s (HPEEKs) that were suitable as rheology control agents for linear poly(ether ether ketone)s (LPEEKs) were first prepared via commercially available hydroquinone (A2 monomer,HQ) and synthesized 3,4',5-trifluoro-benzophenone (BB'2 monomer, TF) effortlessly. The polymerization mode of A2 + BB'2 was demonstrated by MS. Moreover, FTIR, 1H NMR, 13C NMR, and 19F NMR spectroscopy revealed the structure and degree of branching (DB) of the resulting polymers. In comparison with A2 + B3 approach, HPEEK prepared using an A2 + BB'2 strategy possessed a higher molecular weight, and improved the melt processability of LPEEK. Remarkably, with the addition of HPEEK, the mechanical properties of LPEEK increased with a HPEEK content of 5%. The improved mechanical properties were attributed to the good miscibility. Moreover, the addition of HPEEK did not decrease the thermal stability of LPEEK.