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Functional analysis of prv-miR-LLT11a encoded by pseudorabies virus
Huimin Liu,Li Yang,Zhibin Shi,Ruiqi Lv,Xia Yang,Chuanqing Wang,Lu Chen,Hongtao Chang 대한수의학회 2019 Journal of Veterinary Science Vol.20 No.6
Viral-encoded microRNAs (miRNAs) play have vital roles in the regulations of virus replications and host immune responses. The results of previous studies have indicated that miRNA clusters are involved in the replication and virulence of the pseudorabies virus (PRV), which may potentially lead to the immune escape or facilitation of PRV replications. This study's previous research revealed that the prv-mirmiR-LLT11a was differentially expressed during PRV infections. The present study's results have demonstrated that the prv-miR-LLT11a could significantly inhibit PRV replications. It was further determined that SLA-1 was the target gene of the prv-miR-LLT11a, and simultaneously, thate overexpression of prv-miR-LLT11a could down-regulate the mRNA and protein levels of SLA-1 in a dose-independent manner. Furthermore, the present study also found observed that the prv-miR-LLT11a canhad also down-regulated the TAP1 expressions. Our findings provide a better understanding of the molecular mechanism involved in on the effects of prv-miR-LLT11a on SLA-1 and TAP1, as well as and its involvement in a potential immune system evasion of PRV.
Hongmei Yang,Melissa H Jia,Yulin Jia,Junjie Xing,Venu, R-C,Maria Bellizzi,Longping Yuan,Zhilong Wang,Chuanqing Sun,Guo-Liang Wang 한국식물학회 2013 Journal of Plant Biology Vol.56 No.2
Molecular mapping of new blast resistance genes is important for developing resistant rice cultivars using marker-assisted selection. In this study, 259 recombinant inbred lines (RILs) were developed from a cross between Nipponbare and 93-11, and were used to construct a 1165.8-cM linkage map with 131 polymorphic simple sequence repeat (SSR) markers. Four major quantitative trait loci (QTLs) for resistance to six isolates of Magnaporthe oryzae were identified: qPi93-1, qPi93-2, qPi93-3, and qPiN-1. For the three genes identified in 93-11, qPi93-1 is linked with SSR marker RM116 on the short arm of chromosome 11 and explains 33% of the phenotypic variation in resistance to isolate CHE86. qPi93-2 is linked with SSR marker RM224 on the long arm of chromosome 11 and accounts for 31% and 25% of the phenotypic variation in resistance to isolates 162-8B and ARB50, respectively. qPi93-3 is linked with SSR marker RM7102 on chromosome 12 and explains 16%, 53%, and 28% of the phenotypic variation in resistance to isolates CHE86, ARB52, and ARB94, respectively. QTL qPiN-1 from Nipponbare is associated with SSR marker RM302 on chromosome 1 and accounts for 34% of the phenotypic variation in resistance to isolate PO6-6. These new genes can be used to develop new varieties with blast resistance via marker-aided selection and to explore the molecular mechanism of rice blast resistance.