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Chua Kah-Ooi,See-Too Wah-Seng,Tan Jia-Yi,Song Sze-Looi,Yong Hoi-Sen,Yin Wai-Fong,Chan Kok-Gan 한국미생물학회 2020 The journal of microbiology Vol.58 No.12
In this study, bacterial strains Ha5T, Ta1, and Jb2 were isolated from different colonies of weaver ant Oecophylla smaragdina. They were identified as bacterial symbionts of the ant belonging to family Acetobacteraceae and were distinguished as different strains based on distinctive random-amplified polymorphic DNA (RAPD) fingerprints. Cells of these bacterial strains were Gram-negative, rod-shaped, aerobic, non-motile, catalase-positive and oxidase-negative. They were able to grow at 15–37°C (optimum, 28–30°C) and in the presence of 0–1.5% (w/v) NaCl (optimum 0%). Their predominant cellular fatty acids were C18:1 ω7c, C16:0, C19:0 ω8c cyclo, C14:0, and C16:0 2-OH. Strains Ha5T, Ta1, and Jb2 shared highest 16S rRNA gene sequence similarity (94.56–94.63%) with Neokomagataea tanensis NBRC106556T of family Acetobacteraceae. Both 16S rRNA gene sequence-based phylogenetic analysis and core gene-based phylogenomic analysis placed them in a distinct lineage in family Acetobacteraceae. These bacterial strains shared higher than species level thresholds in multiple overall genome-relatedness indices which indicated that they belonged to the same species. In addition, they did not belong to any of the current taxa of Acetobacteraceae as they had low pairwise average nucleotide identity (< 71%), in silico DNA-DNA hybridization (< 38%) and average amino acid identity (< 67%) values with all the type members of the family. Based on these results, bacterial strains Ha5T, Ta1, and Jb2 represent a novel species of a novel genus in family Acetobacteraceae, for which we propose the name Oecophyllibacter saccharovorans gen. nov. sp. nov., and strain Ha5T as the type strain.
Ectopic Expression of a Banana CCCH-Zinc Finger Protein Enhances Salt Tolerance in Arabidopsis
Mazumdar Purabi,Wan Teng Loo,Gwo Rong Wong,Kah‑Ooi Chua,Jennifer Ann Harikrishna 한국식물학회 2023 Journal of Plant Biology Vol.66 No.6
Soil salinisation is a significant challenge to banana production worldwide. Studying candidate salt stress response genes can help to elucidate plant salt-tolerant mechanisms and develop stress-tolerant varieties. In this study, the Musa acuminata Zinc finger15 (MaZFP15) gene, which belongs to the CCCH zinc finger transcription factor family from banana, was introduced into Arabidopsis using the CaMV35S promoter. The transgenic lines expressing MaZFP15 showed increased root branching under salt stress with higher chlorophyll pigment content, lower water loss rates and electrolytic leakage with greater relative water content and increased anti-oxidant enzyme activity (SOD, CAT, POD, APX and GR) compared to control lines (with empty vector) and wild type plants. Analyses of malondialdehyde, the final product of lipid peroxidation, indicated less cell membrane damage in the transgenic lines expressing MaZFP15, than in the control plants. The nuclear location of MaZFP and the elevated expression of abscisic acid-responsive element-binding factor 3 in the salt stress-exposed transgenic lines, support MaZFP15 effecting ABA signalling associated transcriptional regulation of plant stress responses. The responses include the activation of antioxidant enzyme systems and promotion of root growth, contributing to salt tolerance. MaZFP15 can be considered a candidate for molecular breeding of crop varieties that can endure high salt levels.