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      • Resistance Function of Rice Lipid Transfer Protein LTP110

        Ge, Xiaochun,Chen, Jichao,Li, Ning,Lin, Yi,Sun, Chongrong,Cao, Kaiming Korean Society for Biochemistry and Molecular Biol 2003 Journal of biochemistry and molecular biology Vol.36 No.6

        Abstract Plant lipid transfer proteins (LTPs) are a class of proteins whose functions are still unknown. Some are proposed to have antimicrobial activities. To understand whether LTP110, a rice LTP that we previously identified from rice leaves, plays a role in the protection function against some serious rice pathogens, we investigated the antifungal and antibacterial properties of LTP110. A cDNA sequence, encoding the mature peptide of LTP110, was cloned into the Impact-CN prokaryotic expression system. The purified protein was used for an in vitro inhibition test against rice pathogens, Pyricularia oryzae and Xanthomonas oryzae. The results showed that LTP110 inhibited the germination of Pyricularia oryzae spores, and its inhibitory activity decreased in the presence of a divalent cation. This suggests that the antifungal activity is affected by ions in the media; LTP110 only slightly inhibited the growth of Xanthomonas oryzae. However, the addition of LTP110 to cultured Chinese hamster ovarian cells did not retard growth, suggesting that the toxicity of LTP110 is only restricted to some cell types. Its antimicrobial activity is potentially due to interactions between LTP and microbe-specific structures.

      • Construct optimized overlay multicast via preferential random walk

        Xuan Zhang,Xing Li,Chongrong Li 보안공학연구지원센터 2008 International Journal of Multimedia and Ubiquitous Vol.3 No.2

        Tree depth and load balancing are two main metrics in overlay multicast network. Optimizing the two metrics with lightweight overhead is important for live media streaming. This paper proposes one scheme to construct optimized overlay multicast with short tree depth and load balancing via short random walk. The key idea is the preferential random walk based on fitness function in which the tree depth and load balancing metrics are defined as parameters with weighted coefficients. Simulations and experiments show that the fitness function is valid and optimized overlay network could be constructed via preferential random walk. We also find the local and global optimized results occur at some middle value of coefficient between 0 and 1, which is not consist with our intuitions that optimized result with single metric should occur at the boundary of coefficient(0 or 1.0).

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