A Comparison of Protein Pattern in Adventitious and Tap Root Development of Phaselous vulgaris L. : Ⅱ.Adventitious Root Formed from Callus Ⅱ.캘러스로부터 형성된 부정근

CHO, Duck Yee,CHOI, Pil Son,SOH, Woong Young 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

부정근 형성에 있어서 단백질 유형의 변화를 구명하기 위하여 강남콩 하 배축 절편으로부터 유도된 캘러스와 부정근을 분석하였다. 0.8% 한천, 3% 서당, 0.2 mg/L 카이네친 및 0.4 mg/L 2,4-D가 첨가된 MS 기본배지에 배양하여 노란색의 부스러지기 쉬운 캘러스를 얻었다. 이 캘러스를 0.5 mg/L BAP와 0.05mg/L NAA가 첨가된 MS 배지상에 이식 배양한 10 일후 연녹색 캘러스가 형성되었고 뒤이어 녹색의 치밀한 반점의 캘러스가 형성되었다. 이로부터 흑구조가 형성되었고, 이 흑구조에 0.5 mg/L IBA 단독으로 처리하여 부정근을 유도하였다. 이와같은 여러가지 부정근 발달단계에 있어서 단백질 함량을 분석해보면 생중량 1 mg당 노랑색 캘러스에서 5.9μ g/mg, 연녹색 캘러스에서 6.68 g/mg, 녹색반점 캘러스에서 9.65μ g/mg, 흑구조시 7.37 g/mg 및 부정근 형성시 7.02 g/mg이었다. 녹색반점의 치밀한 캘러스에서 단백질 함량이 최고치에 달했으며, 부정근 형성시에는 감소하였다. Laemmli방법으로 행한 1차원 전기영동결과 부정근 유도시에 시간 경과에 따라서 140 KD, 70 kD, 67 kD, 47 kD, 46 kD, 44 kD, and, 43 kD의 특이단백질 밴드가 합성되었다. To investigate the changes of protein patterns during adventiㅅious root formation, protein patterns were analyzed in callus which were induced from hypocotyl explant of Phaseolus vulgaris. Yellowish friable callus was induced from the explant cultured on Murashige-Skoog basal medium supplemented with 0.2 mg/L Kinetin and 0.4 mg/L 2,4,-D. This callus was transferred on MS medium supplemented with 0.5 mg/L BAP and 0.05 mg/L NAA. After 10 days culture, the callus showed pale green color and then green compact spots which turned to nodular structures appeared on the callus. Finally, adventitious roots developed from the nodular structure, when the culture was transferred to MS medium containing 0.5 mg/L IBA. The protein content was 5.94 μ g/mg fr wt in yellowish callus, 6.68 μ g/mg fr wt in pale green callus, 9.65μ g/mg fr wt in green spot callus, 7.37μ g/mg fr wt in nodular structure, and 7.02μ g/mg fr wt during adventitious root formation respectively. The green compact spot on the callus contatined maximum quantities of proteins while the protein content decreased during adventitious root formation from the nodular structure. From one-dimensional SDS-PAGE, several protein bands could be identified which were specific for adventitious roots at various developmental stages: 140 KD, 70 kD, 67 kD, 64 KD, 47 kD, 46 kD, 44 kD, and, 43 kD polypeptides were the most specific.

Carbohydrate metabolism in adventitious root formation on Phaseolus vulgaris L . hypocotyl cuttings

Cho Duck Yee,Seong Gil Kim,Hak Su Kim,Young Soon Kim,Woong Ypung Soh 한국식물생명공학회(구 한국식물조직배양학회) 1993 한국식물생명공학회 춘계학술연구발표회 Vol.1993 No.-

Anomalous Structure of Somatic Embryos Developed from Leaf Explant Cultures of Angelica gigas Nakai

Cho, Duck-Yee,Lee, Eun-Kyong,Soh, Woong-Young The Korean Society of Plant Biotechnology 1998 식물생명공학회지 Vol.25 No.1

당귀(Angelica gigas Nakai)의 잎절편유래 체세포배의 비정상자엽구조에 관한 2,4-D와 BA의 영향에 대하여 또한 체세포배의 자엽수와 발아와의 관계에 대하여 기술하였다. 배발생능 캘러스는 0.5 및 1 mg/L 2,4-D와 1mg/L 2,4-D+0.1mg/L BA, 1mg/L 2,4-D+0.5mg/L BA가 첨가된 MS 기본배지에 치상한 외식편에 형성된 캘러스로부터 선발하였다. 1mg/L 2,4-D첨가 MS고형 기본배지에서 8 주간 배양후 2,4-D가 제거된 배지에서 3주간 배양하여 체세포배 발생과 비정상적인 체세포배의 자엽발생을 관찰하였다. 이상자엽의 빈도는 정상인 2개의 자엽을 갖는 체세포배는 42.5%인데 반하여 1개의 자엽에서 22.8%, 3개의 자엽을 갖는 체세포배에서는 16.8%, 4개의 자엽에서는 7.8%, 5개의 자엽배에서는 1.8%와 주발형 자엽배는 8.2%이었다. 또한 ABA를 처리하면 2개의 자엽을 갖는 체세포배는 65.3%로 향상되었다. ABA 는 정상적인 체세포배의 높은 생산에 중요하였다. 2개의 정상적인 자엽을 갖는 체세포배의 발아율은 77.8%이었으나 비정상적인 자엽을 갖는 체세포배의 발아율은 대단히 낮아서 1개, 3개, 4개, 5개 및 주발형의 자엽배을 갖는 체세포배에서는 62.5%, 43.3%, 60%, 50%및 0%이었다. This study describes the effect of the growth regulators such as 2,L-D and BA, on the structural abnormalities of somatic embryos derived from leaf explants of Angelica gigas Nakai, Also, the relationship between the cotyledon number of a somatic embryo and its germinability is explored. Embryogenic calli were selected from calli formed on explants cultured on MS solid basal medium supplemented with 0.5mg/L 2,4-D, 1mg/L 2,4-D, 1mg/L plus 0.1mg/L BA, and 1 mg/L 2,4-D plus 0.5mg/L BA. Cotyledonary abnormalities were observed in somatic embryos which were developed from embryogenic calli cultured on MS medium containing 1mg/L 2,4-D for 8 weeks and then subcultured on 2,4-D free MS medium for 3 weeks. The frequency of abnormalities was as follows: 22.8% one cotyledon, 42.5% two cotyledons, 16.8% three cotyledons, 7.8% four cotyledons, 1.8% five cotyledons, and 8.2% jar shaped cotyledon. In addition, ABA treatment indicated an improvement of the somatic embryo with normal cotyledon (65.3%). ABA was important role to the high production of normal somatic embryos. Two cotyledon embryos showed germinability 77.8%. However the germinability of somatic embryos with anomalous cotyledons was prominently low: One cotyledon, 62.5%; three cotyledons, 43.3%; four cotyledons, 60%; five cotyledons, 50% and jar shaped cotyledon, zero%. Thus, germinability was essentially, inversely proportional to cotyledon number.

A Comparison of Protein Pattern in Adventitious and Tap Root Development of Phaseolus vulgaris L. : Ⅰ. Adventitious Root from Hypocotyl Cuttings and Tap Root of Germination Seed Ⅰ. 하배축 절편으로부터 형성된 부정근과 발아종자의 주근

CHO, Duck Yee,CHOI, Pil Son,SOH, Woong Young 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

This work was carried out to elucidate de novo protein synthesis and patterns during the root development of germinating seed and the adventitious root formation of Phaseolus vulgaris hypocotyl cuttings in light or dark incubation. One-dimensional SDS-PAGE of a cell fraction was performed as described by Laemmli. Protein contents of hypocotyl cuttings were increased to 9.62 μ g/mg fr wt during the period of root primordia induction after 96 h culture, while the content was decreased 7.45μ g/mg fr wt during adventitious root formation. The change of protein content was the same as that of cuttings incubated in dark. Protein content from the tap root of germinating seed after 72h cultrue was decreased 69.49% in comparison to the radicle in the seed. The protein content was decreased during root elongation occurred in both the adventitious root on hypocotyl cuttings and the tap root of the germinating seed. One-hundred-and-ninty-four KD, 127 KD, 83 KD, 81 KD, 51 Kd and 43 Kd were specifically synthesized during adventitious root formation from hypocotyl cuttings in light or dark incubation. Thirty-three polypetides in the tap root from germinating seed were specifically synthesized. Among them 43 KD polypeptide was the common with adventitious root from hypocotyl cuttings.

Effect of Endogenous IAA Transport on Adventitionus Root Formation in Phaseolus vulgaris Hypocotyl Cuttings

Cho, Duck Yee,Woong, Young Soh 全北大學校 基礎科學硏究所 1991 基礎科學 Vol.14 No.1

강남콩 하배축 절편에 있어서 부정근형성과 내재 및 외래 IAA 이동에 관해서 실험하였다. 도립 및 정상배양에 있어서 언제나 형태적 기부에서 부정근형성이 일어났다. 도립배양의 결과 초기 24 시간(0-24hr)에 더욱 억제적 효과를 보였고 PCIB의 처리에서도 같은 경향을 보였다. TIBA처리에서는 현저하게 부정근형성을 억제하였고 이 억제적 효과는 처리시간에 비례하며 근 신장기보다는 근 원기에 더욱 억제효과가 컸다. PCIB와 TIBA의 오옥신 이동억제효과는 외래 IAA용액 처리에 의해서 회복 되었다. This work was carried out to elucidate effects of endogenous and exogenous IAA transport on adventitious root formation in Phaseolus vulgaris gypocotyl cuttings. For inverted or normal incubation in distilled water, the adventitious root is always formed at the morphological base but not at the morphological apex. For inverted incubation, in both distilled water and certain chamical solution, the root formation is retarded more at the first stage (0-24 hr) than at the second stage (24-48 hr). when p-chlorophenoxyisobutyric acid (PCIB) was applied to the cuttings at the first stage, the root formation was inhibition more than at the second stage. Treatrment of 2,3,5-triiodobenzoic acid (TIBA)makedly influenced the root according to the applied stage and period. Therefore, the root formation is more related to the stage of root primordium formation than to the stage of root elongation from the primordium. Inhibition of auxin transport or action by TIBA or PCIB could also be reversed when hypocotyl cuttings are incubated in exogenously applied IAA solution.

강활 잎절편 배양으로부터 체세포배의 발생 및 발아에 미치는 배지 산성도의 영향

CHO Duck-Yee,SOH Woong-Young,LEE Eun Kyung 한국자원식물학회 2000 한국자원식물학회 학술심포지엄 Vol.2000 No.-

강남콩 하배체 (下胚體) 절편의 (切片) 부정근형성에 미치는 내재 IAA 의 이종

조덕이(Duck Yee Cho),소웅영(Woong Young Soh) 한국식물학회 1989 Journal of Plant Biology Vol.32 No.4

This work was carried out to elucidate effects of endogenous and exogenous IAA transport on adventitious root formation in Phaseolus vulgaris hypocotyl cuttings. For inverted or normal incubation in distilled water, the adventitious root is always formed at the morphological base, but not at the morphological apex. For inverted incubation, in both distilled water and certain chemical solution, the root formation is retarded more at the first stage (0-24 hr) than at the second stage (24-48 hr). When p-chlorophenoxyisobutyric acid (PCIB) was applied to the cuttings at the first stage, the root formation was inhibited more than at the second stage. Treatment of 2,3,5-triiodobenzoic acid (TIBA) markedly inhibited the adventitious root formation in Phaseolus vulgaris hypocotyl cuttings. This inhibition influenced the root according to the applied stage and period. Therefore, the root formation is more related to the stage of root primordium formation than to the stage of root elongation from the primordium. Inhibition of auxin transport or action by TIBA or PCIB could also be reversed when hypocotyl cuttings are incubated in exogenously applied IAA solution.

시호(Bupleurum falcatum L.) 잎절편체로 부터 부정아 형성을 통한 식물체 재생

조덕이,이은경 우석대학교 자원개발연구소 1997 자원개발연구소논문집 Vol.2 No.-

This study was carried out plant regeneration via adventitious bud formation from leaf explant of Bupleurum falcatum L. Leaf explants were cultured on MS medium supplemented with various concentrations (0,0.1,0.5,1,3,5 mg/L) of 2,4-D and BA in combination or soley treatment. After 6 weeks 2,4-D medium and pale green callus was induced with 0.1 mg/L 2,4 D+ 0.1mg/L BA. Adventitious bud was transferred onto half strungth Ms basal medium for 3 weeks of culture, adventitious roots formed and then plantlets were produced. The treatment of high concentration (2,3,5 mg/L) of BA, callus occurred necrosis.

오이(Cucumis sativus L.) 자엽절편으로부터 부정근 형성에 관한 Indole-3-butyric acid 와 6-benzyl adenine의 영향

조덕이,최인종 우석대학교 자원개발연구소 1996 자원개발연구소논문집 Vol.1 No.-

본 연구는 오이 자엽절편의 부정근 형성에서 IBA 및 BA의 영향을 알아보기 위하여 해부학적 및 생화학적 연구를 수행하였다. 자엽절편을 MS기본배지, IBA + BA 및 IBA, BA단독배지에서 1개월간 배양하여 Laemmli방법에 의해서 1차원 전기영동을 실시하였다. 0.5 mg/L BA첨가 MS배지에 배양한 자엽의 단백질 함량은 1.474 ㎍/mg.fr.wt.이었으나 3mg/L IBA처리시, 부정근 형성이 일어났고 이때 1.014㎍/mg.fr.wt로서 함량이 감소하였다. 64.7 KD, 58.8 KD, 32.7 KD 및 28.8 KD등의 특이밴드가 합성되었다. 또한 부정근 원기형성은 3mg/L IBA 첨가 MS배지에서 1일간 배양시 유관속 형성층과 사부유조직으로부터 시원세포가 형성되었고 3일간 배양할때 부정근 원기가 형성되어 표피쪽으로 원형의 분열조직을 형성하여 배양 5일 후 근신장을 하였다. This work was carried out to elucidate de novo protein synthesis and anatomical research during the adventitious root formation from cotyledon explant of Cucumis sativus L. One-dimensional SDS-PAGE of a cell fraction was performed as described by Laemmli. Protein contents of cotyledon were increased 1.474 ㎍/mg.fr.wt. for MS media supplemented with 0.5 mg/L BA, while the contents were decreased 1.014 ㎍/mg.fr.wt. during adventitious root formation on MS media containing 3 mg/L IBA media. 64.7 KD, 58.8 KD, 32.7 KD and 28.8 KD were specifically synthesized during adventitious root formation from Cucumis sativus cotyledon culture. The adventitious root initials were originated from vascular cambium and phloem parenchyma tissue on MS media supplemented with 3 mg/L IBA for one day of culture. And then root primordia were made by three day of culture, and also adventitious root elongation toward the cotyledon epidermis after five days of culture.

팔의 상배축 절편에 있어서의 부정근 형성에 미치는 PCIB와 카드뮴의 영향

趙德以 又石大學校 1982 論文集 Vol.4 No.-

Adventitious root formation in epicotyl cuttings of light-grown seedlings of Phaseolus angularis was studied. The initiation of roots arised at the basal 2㎜ portion when the basal parts of the cutting was dipped in water and testing solutions. P-chlorophenoxyisobutyric acid (PCIB) at the optimum concentrations promoted rooting of epicotyl cuttings. In combination with IAA, PCIB has mostly suppressed promoting effect of IAA. Cadmium chloride (Cdcl_2) markedly decreased the number of roots of cuttings treated with IAA or singly. IAA did overcome the inhibitory effect of Cdcl_2 on adventitious root formation. Cdcl_2 showed strong inhibition of both primordia initiation and emergence.