Bromophenol Blue에 依한 亞窒酸 Ion의 吸光度定量法

崔七男 조선대학교 기초과학연구소 1978 自然科學硏究 Vol.1 No.1

特別한 裝置나 複雜한 操作없이 簡單하고 迅速한 Bromophenil Blue에 의한 亞窒酸이 온의 吸光光度 定量法을 定하였다. 檢液을 Bromophenil Blue試液과 30N-黃酸으로 70℃에서 30分間 反應시키면 安定하고 再現性이 좋은 發色을 얻을 있으며 580mm에서 亞窒酸性窒素의 濃度가 극히 적은 範圍까지도 吸光度와 直線的 關係가 있고 反應 系에서 吸光種의 數는 單 하나임을 確認하였다. A simple and rapid Spectrophotometric method has been found for the determinatin of nitrite content in natural water with Bromophenol Blue method by means of neither particular apparatus nor troublesome procedurces. Bromophenol Blue reacted with nitrite in the presence of 30N-sulfuric acid to develop stable color with good reproducibility after the reaction of 30 minutes at 70℃. An essentially linear relationship of the nitrite concentration was observed of Bramophenol Blue in 580nm, and it was established that the number of absorbing species in the reaction system was only one between 500nm and 580nm.

Alizarin Yellow에 依한 微量 As의 吸光度 測定

崔七男,丁梧鎭 조선대학교 기초과학연구소 1979 自然科學硏究 Vol.2 No.1

This paper is attempted to discuss an analysis of a sample and rapid Spectrophotometric method without particular apparatus or difficult procedures. We dropped 1 ml of Alizarine Yellow solution into 25 ml of the Arsenic solution and that PH was adjusted to 10 with Sodium Hydroxide. At PH 10, alizarin yellow reacted with Arsenic developed the stable color with good reproducibility after an hour in water at 25℃. An essentially linear relationship to the Arsenic was observed in the range of 1 ㎍-40 ㎍ at 745 nm, an it was established that the number of absorbing species in the reaction system was only between 700 nm and 750 nm. Among the interferences. arsenic material could be removed by the addition of metanol. One hour's standing did not chnge a bit in the absorbance. The mole ratio of Alizarin Yellow in the complex under given condition was 1:1.

大學 入學試驗 本 考査 化學問題에 對한 考察

曺在興,崔七男,魯鳳五 조선대학교 교육연구소 1980 교과교육연구 Vol.3 No.-

There are two basic approaches concerning the analysis of the distribution of items on the chemistry tests that were presented in the matriculation for University students during the five years (1974~1975) in Korea. One approach compares the subjective contents of the items with their behavioral objects, and the second approach compares the subject contents with the type of activity elicited by the items (Knowledge,, Calculation, Thinking, and Experiment) The results of the two classifications were as follows: 1. Items testing the subject contents were distributed over basic concepts for a section of each unit but there was no constructive item of a related unit. 2. The evaluated items concerning the faculty of scientific investigation and the procedure of experimentals that were asked were either few or were not included at all. 3. The items testing application sills were only 13%. 4. The number of multiple choice question was 27% and fill in the blank and complete the sentence questions are asked in equal numbers

포름산-물, 아세톤-물, 및 에탄올-물 혼합용매 중에서 철족 염화물의 거동에 관한 연구 : Formic Acid-Water, Aceton-Water and Ethanol-Water

김일두,최칠남,정오진 조선대학교 기초과학연구소 1980 自然科學硏究 Vol.3 No.1

The electric conductances of iron, cobalt and nickel chloride in a series of some mixed solvent, formic acid-water, acetone-water and ethanol-water, have been measured at 30℃, respectively. The limiting equivalent cnductances of the salts have been computed by Debye-Hukel-Onsager, and various limiting equivalent conductances with the compositions in each of mixed solvents. have been explained in terms of selective solvation of electrolytes. The limiting ionic conductances of Fe^+2, Co^+2 and Ni^+2 are in order of Ni^+2<Co^+2<Fe^+2 which is the reversed order of solution for the ions in dilute solution. 2) The values of FeCl_2, CoCl_2 and NiCl_2(0,0∼0.3 mole fraction) in acetone-water mixed solvent are in order of NiCl_2<CoCl_2<FeCl_2 but in order of NiCl_2<FeCl_2 <CoCl_2 in more than 0.4 mole fraction. 3) For thes state of dilute solution, the chlorides of Fe-group is selectively solvated water rather than acetone and ethanol. 4) According to the increase of the mole fraction of acetone in mixed solvent, Walden products (A。??。values) of electrolytes are decreased. 5) Limiting equivalent conductances of 10^-2-10^-4 mole 1^-1-FeCl_2, CoCl_2 and NiCl_2 that are analyzed in ethanol-water and acetone- water mixed solvent at 30℃, is agree with calculated values by Debye-Hukel-Onsager equation. 6) The conductances of dilute solution of FeCl_2 and NiCl_2 in formic acid-water mixed solvent could not be analyzed because of strong ionic atmosphre of solvent itself.

Chromatography를 이용한 한국산 Comfrey의 Amino acid 분리 및 정량

曺在興,崔七男,丁梧鎭 조선대학교 기초과학연구소 1978 自然科學硏究 Vol.1 No.1

Camfrey의 잎과 뿌리에 함유된 free amino acid의 종류와 함량을 G.L.C와 T.L.C로 측정한 결과는 다음과 같다. ① GLC에 의해서 검출한free amino acid는 13종이었으며 β-alanine, Valine, glycine, Isoleusine, Methionine, Aspartic acid, Glutamine, Lysine, Arginine, Histidine, Cystine, Triphtophan, Phenylamine등 13종이 정량되었으며 이들중 Argine은 전질소 100% 중에서 10.77%로 제일 많았고 그 다음이 Glutamic acid와 Aspartic Acid, Alanine, Lysine 순이었다. ② T.L.C.로 검출한 amino acid는 G.L.C.로 검출한 것보다 Leucine, Tryptophane, Cysteic acid를 검출할 수 있었으며 定量的인 면에서도 G.L.C. 방법과 일치하였으나 검출감도가 나쁘고 1종은 미확인 되었다. ③ G.L.C.의 분석은 T.L.C.분석보다도 검출감도가 높고 정량적이었으나 재현성이 낮았고 실험과정이 복잡하여 비용이 많이든다. ④ Camfrey 중에서 잎과 뿌리에 함유된 amino acid의 함량과 성분을 비교해볼때 잎보다도 뿌리에 Amino acid의 함량과 종류가 약간 많았다. ⑤ Camfrey에는 많은 amino acid가 함유되어 있는것으로 보아 동물의 사료용으로 광범위하게 쓰일 수 있으며 Comfrey 차 이외의 다른 가공식품을 만들 수 있으리라고 사료된다. Amino acids of comfrey produced in Korea have been analyzed exclusively by gas chromatogram of Gas Chromatograph (Shimadzu, GC-1C) equiped with F.I.D. and have been identified by T.L.C. Through this analysis, the following results were obtained. 1. The quantities of free amino acids in root of comfrey analyzed by T.L.C. were determined 10.23% arginine, 7.86% glutamic acid, 7.21% aspartic acid, 6.08% alanine, 4.87% lysine 4.025% histidine, 2.90% glysine, 2.86% cysteic acid, 2.52% valine, 1.68% phenylamine, 1.52% isoleucine, 1.42% leucine, 1.25% threanine, 1.05% nethionine, 0.58% cystein, 0.56% tryptophane 2. The quantities of free amino acids in leaf of comfrey analyzed by G.L.C. were determined 9.56% arginine, 6.52% aspartic acid, 6.21% glutamic acid, 5.82% alamine, 4.02% lysine, 3.45% histidine, 2.54% glysine, 1.96% valine, 1.52% isoleusine, 1.26% threanine, 1.20% phenylamine, 0.68% methiomine and 0.21% cysteine 3. The quantities of free amino acids in comfrey were determined more at root than at leaf and almost all the essential amino acids were analysed by G.L.C.

디메틸술폭시드 용매중에서 거대고리리간드를 포함한 우라늄(VI)과 희토류(III) 금속 착물의 폴라로그램

정학진,정오진,최칠남,Hak Jin Jung,Oh Jin Jung,Chilnam Choi 대한화학회 1988 대한화학회지 Vol.32 No.3

디메틸술폭시드 용매 중에서 아직 밝혀진 바 없는 불포화계 $N_2O_2$형 거대고리 리간드들을 포함한 우라늄(VI)과 네오디움(III)착물, 그리고 cryptand 222와 DBC 거대고리 리간드를 가지는 네오디움(III)과 우라늄(VI)착물에 대한 직류폴라로그래프적 거동을 조사하였다. 직류폴라로그래피에 의하여 리간드와 착물의 산화 환원성, 가역성, 환원과정에 관여하는 전자수 및 산첨가에 따른 영향 및 산화-환원전극반응의 메카니즘을 알아 보았다. 그리고 착물들의 안정도 상수와 조성비를 알아보았다. immine계 거대고리 리간드를 가지는 네오디움(III)착물의 환원파는 나타나지 않았으나, 우라늄(VI)착물은 6전자 3단계 환원파를 DBC를 가지는 우라늄(VI)착물은 2전자 2단계 환원파를, 그리고 cryptand 222와 DBC를 가지는 네오디움(III)착물은 1전자 1단계 환원파를 각각 나타내었으며 이들 착물들은 확산지배적이며 가역성은 비교적 좋았다. uranyl(II)금속 착이온의 제 1,2단계 환원파는 1전자 환원반응이며 제 3단계 환원파에서는 4전자 환원반응, 그리고 neodymium(III)착물은 1전자 1단계 환원과정을 가진다. DBC와 imine계 거대고리 리간드를 가지는 우라늄(VI)착물은 pH=7.0이상에서, cryptand 222와 DBC를 리간드로 하는 네오디움(III)착물은 pH=4.0 이상에서 안정한 착물을 형성한다. imine계 거대고리 리간드를 가지는 $UO_2\;^{2+}$착물의 안정도 상수값은 $7.008{\sim}7.273$이라는 새로운 안정도 상수값들을 얻었고 이 값들은 Odien-Ntn > Trans Otn-Ntn = Cis Otn-Ntn > Oen-Ntn 순이었다. 그리고 $UO_2(II)$-cryptand 222 및 Nd(III)-DBC 착물의 안정도 상수값은 7.614, 12.669, 4.223이었고 DBC리간드 착물에서는 $UO_2\;^{2+}$착물의 안정도 상수값이 $Nd^{3+}$의 그것보다 크며 $Nd^{3+}-cryptand\;222$ 착물의 안정도 상수가 가장 크다. The uranium(VI) complexes with new unsaturated macrocyclic ligands of cryptand types and the neodymium(III) complexes with cryptand 222 and DBC ligands have been investigated polarographically in dimethylsulfoxide solvent. The reduction states, electron numbers involved in the reduction process, effects of the added acid on the polarograms of complexes, and the mechanisms of the reduction electrode reactions have been examined. The stability constants and mole-ratio of new complexes were also obtained by polarographic method. The reaction of ligands was controlled by the diffusion in the reduction with four electrons at a step, whereas the redox reaction with six electrons at three steps in $UO_2\;^{2+}$ complexes with macrocyclic ligands and the redox reaction with one electron at a step in $Nd^{3+}$ complexes with cryptand 222 and DBC have been observed. The imine ligands formed stable complexes with uranium(VI) above pH 7.0, and the neodymium(III) complexes with cryptand 222 and DBC ligands were stable above pH 4.0.