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      • The capsular polysaccharide of pyogenic liver abscessKlebsiella pneumoniae induces maturation and inflammatory response of human monocyte-derived dendritic cells

        Kuo-Feng Hua,Chia-Yang Li,Feng-Ling Yang,Shih-Hsiung Wu 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1

        The capsular polysaccharide (CPS) of pyogenic liver abscessKlebsiella pneumoniaeconsists of repeating units of the trisaccharide (→3)-□-D-Glc-(1→4)-[2,3-(S)-pyruvate]-□-D-GlcA-(1→4)-□-L-Fuc-(1→) and has the unusual feature of extensive pyruvation of glucuronic acid and acetylation of C2-OH or C3-OH of fucose. The present study investigates how CPS activates human monocyte-derived dendritic cells (DCs). Our experimental results show that CPS activates DCs by (1) increasing the expression of CD11c, CD40, CD80, CD83, CD86, and MHC-II (2) increasing the production of TNF-□, IL-1, IL-6, and IL-12p70 (3) increasing DC-elicited allogeneic T-cell proliferation, and (4) increasing the DC-driven Th1 response. In addition, CPS activates DCs through TLR4 and the pyruvation and the acetylation of CPS are important for its cytokine induction activity. Further, our results show that CPS activates TNF-□ and IL-6 secretion through JNK1/2, p38, NF-B,- PKC and ROS pathways in DCs.

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        Life cycle of the aquatic firefly Luciola ficta (Coleoptera: Lampyridae)

        Jen-Zon Ho,Pi-Hei Chiang,Chia-Hsiung Wu,Ping-Shih Yang 한국응용곤충학회 2010 Journal of Asia-Pacific Entomology Vol.13 No.3

        This research is the first to record the complete life history of the aquatic firefly Luciola ficta (Olivier) using a unique individual rearingmethod. Transparent containers (250 ml; height: 6 cm; bottom diameter: 8 cm; mouth diameter:9.5 cm) were used to rear individuals fromegg to adult in the laboratory, so that they could be observed throughout thewhole life cycle. Larvae were fed on themeat of thewater snail Cipangopaludina chinensis (Gray). Temperature ranged from18 °C to 30 °C, relative humidity (RH)was 80±5%, and the light:dark (L:D) ratiowas 10:14. Of 80 eggs,35 individuals completed their life cycle under these laboratory conditions in Jiji, Nantou County, Taiwan. The external morphological characteristics of each growing stage were described. Egg hatching rate was 95%. On average, one generation spanned 388.2±25.7 days. The durations of egg, larva, climbing larva, cocoon, and adult stages were 19.1±1.5 days, 328.9±33.2 days, 10.9±7.8 days, 14.7±5.3 days, and 15.7±5.2 days, respectively. The number of larval instars ranged fromfive to seven,with amodal value of six instars for males and seven instars for females. Female larval duration averaged 337.1±31.2 days, whichwas higher than the 307.6±34.1 days of the males. From January to December 2002, adult emergence peaked twice, with themain high peak appearing in April and the second peak occurring in August. The results of indoor rearing and of field investigations in Jiji, Nantou County, suggested that L. ficta is univoltine.Adult body length is negatively correlatedwith larval duration (Pb0.01). The life history traits of L. ficta show plasticity in adult occurrence, egg size, egg duration, larval duration, larval instars, and adult body length. Some variations were discussed in the context of survivorship in field habitats.

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        Application of physical vapor deposition process to modify activated carbon fibers for ozone reduction

        Yu-Chih Lin,Chung-Liang Chang,Tser-Sheng Lin,Hsunling Bai,Ming-Gu Yan,Fu-Hsiang Ko,Chia-Tien Wu,Cheng-Hsiung Huang 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.3

        This study utilized the activated carbon fiber (ACF) modified with metal catalyst via physical vapor deposition (PVD) process (ACF/PVD) to diminish ozone. Furthermore, the ozone removal efficiency of ACF/PVD was compared with that of original ACF and ACF modified with metal catalyst via impregnation process (ACF/impregnation). In addition to the kinds of coated metal and the inlet ozone concentrations, the effects of the coating thickness and the reaction temperature on ACF/PVD for ozone removal were also examined. The results indicate that the ozone removal efficiency of ACF/PVD is better than that of original ACF and ACF/impregnation. The ozone removal efficiency of different metal-coated ACF/PVD in the superior order is gold (Au), and manganese (Mn). The increase of Au-coated thickness (3 nm to 80 nm) on ACF/PVD will enhance the ozone removal. However, when the Mn-coated thickness on ACF/PVD is larger than 15 nm, the ozone removal efficiency displays a declining trend. Furthermore, a higher reaction temperature will result in a better ozone removal of ACF/PVD and the original ACF.

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