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PCR-Based Detection of Densovirus Infection in Silkworm (Bombyx mori L.)
Hou Chengxiang,Li Muwang,Gui Zhongzheng,Xu Anying,Guo Xijie Korean Society of Sericultural Science 2005 International Journal of Industrial Entomology Vol.11 No.2
Two pairs of DNA primers were designed for the detection of the Zhenjiang (China) strain of Bombyx mori densonucleosis virus (BmDNV-Z). These primers were designed from the nucleotide sequence of major structural protein gene (putative VD1-ORF2). PCR amplification was attempted from different issues (including silk gland, blood, skin and midgut) and feces of the silkworm which infected wit BmDNV-Z were amplified by PCR. Both of the primers gave expected size of in the DNA bands from midgut and feces, but not in the DNA of silk gland, blood and skin. The two bands were sequenced, and their sequence were same as the sequence designed for. BmDNV-Z could be successfully detected in single silkworm after it was infected for 12 hrs, and could not be detected before 9 hrs after infected.
Tao Geng,Yuxia Huang,Chengxiang Hou,Guangxing Qin,Dingding Lv,Xijie Guo 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3
Innate immunity is critical to insects and plays an important role in pathogen elimination and wound healing. Toll signaling pathway is the major signaling pathway associated with insect innate immunity mediating synthesis of anti-fungal/bacterial peptides. To better understand the Toll signaling pathwaymediated immune response in Bombyx mori against Beauveria bassiana infection, expression patterns of genes encoding sixteen putative components of Toll signaling pathway in the silkworm larvae challenged with B. bassiana, including four pattern recognition receptors (PRRs, i.e. BmβGRP 1, 2, 3, 4), eight Toll-like receptors (TLRs, i.e. Bm18w, BmToll 1, 3, 6, 9, 7, 10, 11) and four effectors (BmMoricin 1, BmGloverin 2, BmDefensin 1 and BmLysozyme 1), were analyzed using quantitative real-time PCR. At the same time, the changes in their expression by RNAi knock-down of the four PRRswere also detected.Moreover, the effects of Toll signaling pathway inhibitors on antifungal activity in larvae hemolymphwere also analyzed. The results showed that the expression levels of genes encoding sixteen putative components of Toll signaling pathway were obviously altered by the challengewith B. bassiana, but their temporal regulation mode was significantly different. Based on the expression patterns of the genes related to Toll signaling pathway, two sub-paths of immune signal recognition and transduction might be proposed in the response of silkworm larvae against B. bassiana infection. Besides, Toll signaling pathway inhibitor could significantly inhibit the antifungal activity in hemolymph and resulted in increased sensitivity of silkworm larvae to the B. bassiana infection, while the treatment with heat-inactivated B. bassiana could induce antifungal activity in the hemolymph and led to stronger resistance of the silkworm. These results implied that Toll signaling pathway played important roles in the antifungal immune response of the silkworm larvae, in which different components of Toll signaling pathway might play a specific regulatory function. These findings yield insights into the innate immune mechanisms underlying Toll signaling pathway in silkworm.
Xu, Anying,Lin, Changqi,Hou, Chengxiang,Zhang, Yuehua,Li, Muwang,Sun, Pingjiang Korean Society of Sericultural Science 2006 International Journal of Industrial Entomology Vol.13 No.1
The major dominant fluoride-endurance (Dfe) gene was introduced into the commercial varieties by crossing and pedigree selection to breed silkworm races that could normally develop in the area that polluted by fluoride. After backcrossed for two generations, the Dfe gene was made homozygous, and individuals with good economic characters were selected to generate next generation. After 8 generations of selection, their characters became stable, and the silkworm variety which is resistant to fluoride, Huayuan${\times}$Dongsheng, for spring rearing were bred.
OsSPL13 controls grain size in cultivated rice
Si, Lizhen,Chen, Jiaying,Huang, Xuehui,Gong, Hao,Luo, Jianghong,Hou, Qingqing,Zhou, Taoying,Lu, Tingting,Zhu, Jingjie,Shangguan, Yingying,Chen, Erwang,Gong, Chengxiang,Zhao, Qiang,Jing, Yufeng,Zhao, Y Nature Publishing Group, a division of Macmillan P 2016 Nature genetics Vol.48 No.4
<P>Although genetic diversity has a cardinal role in domestication, abundant natural allelic variations across the rice genome that cause agronomically important differences between diverse varieties have not been fully explored. Here we implement an approach integrating genome-wide association testing with functional analysis on grain size in a diverse rice population. We report that a major quantitative trait locus, GLW7, encoding the plant-specific transcription factor OsSPL13, positively regulates cell size in the grain hull, resulting in enhanced rice grain length and yield. We determine that a tandem-repeat sequence in the 5'UTR of OsSPL13 alters its expression by affecting transcription and translation and that high expression of OsSPL13 is associated with large grains in tropical japonica rice. Further analysis indicates that the large-grain allele of GLW7 in tropical japonica rice was introgressed from indica varieties under artificial selection. Our study demonstrates that new genes can be effectively identified on the basis of genome-wide association data.</P>