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RISS 인기검색어
- 검색키워드
- 저자 : Changsheng Fan (검색결과 4 건)
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Research on Preparation Methods of Ultrafine Softwood Powder
Changsheng Fan,Dongxia Yang,Hongling Wang,Yan Sun,Hua lou,Hongru Yang 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.4
Pine wood sawdust is used as raw materials for experimental processing into ultrafine wood powder. Sizes of ultrafine wood powder particles serve as a standard for measuring ultrafine processing. The core part of the experimental processing equipment is the millstone which can exert great shear force and grinding force on strong fiber materials so as to ensure the successful preparation of ultrafine particles. The “equilibrium orbit” model is used to simulate processed superfine particles in calculating separation performance. Moreover, the CFD is chosen for simulating separation performance of wood powder particles with different sizes in the two-phase flow field, so as to ensure the successful separation and grading of wood powder particles with different sizes in the practical processing. Research is made on physical properties of collected wood powder as samples with different sizes and influence on composite material properties when ultrafine wood powder is taken as padding. Research on ultrafine wood powder provides meaningful experimental data and theoretical support for the future research on micro-nano fibrils.
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Study of Hair Melanins in Various Hair Color Alpaca (Lama Pacos)
Fan, Ruiwen,Yang, Gang,Dong, Changsheng Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.4
The aim of this study was to measure the hair melanins of various colors and to find the relationship between the quantity of melanins and hair color phenotypes in alpacas. According to the Munsell color system, 3 healthy alpacas were selected for each of the 22 different hair color phenotypes (66 alpacas altogether). Alpaca hair was taken from the lateral thoracic region and then dissolved with different solutions to obtain melanins. The values of alkali-soluble melanins (ASM), eumelanin (EM) and pheomelanin (PM) were measured by spectrophotometric assay, and labeled as Sp.ASM, Sp.EM and Sp.PM, respectively. Data were analyzed using SPSS11.5 software. Results showed that average Sp.ASM and Sp.PM were increased as the color deepened from white to black, ranging from 0.500 to 4.543 for Sp.ASM and from 0.268 to 1.457 for Sp.EM. However, average Sp.PM had no such apparent relationship with color. Based on the value of Sp.ASM and EM, 7 hues were produced and gray was a single hue. Most of the data were in a normal distribution (p>0.10). ANOVA analysis showed that mean values of Sp.ASM, Sp.EM and Sp.PM were significantly different (p<0.05). The results also showed that Sp.ASM was positively correlated with Sp.EM but the correlation between Sp.ASM and Sp.PM was not significantly different from 0. It is concluded that EM is the major constituent of alpaca hair melanin; there is a significant correlation among ASM, EM and alpaca hair colors, and EM is the most reliable parameter for distinguishing these groups.
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Lou Tengfei,Chen Kai,Luo Qiyu,Liu Changsheng,Yuan Yuan,Fan Cunyi 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00
Distraction osteogenesis (DO) is an efficacious but lengthy procedure to reconstruct segmental bone defects under the principle of tension-stress, during which the periosteum-mediated mechanical stimulation plays a pivotal role. Inspired by the dynamic process of DO and the mechanical stimulation of periosteum, a new design of bionic periosteum was developed to simulate the mechanical transduction of natural periosteum for the application in DO procedure.In this study, an injectable organic-inorganic hybrid hydrogel was developed based on a novel combination of the PEGylated poly (glycerol sebacate) (PEGS) polymer network and in situ formed CaP nanoparticles (ICPNs). Rat bone marrow mesenchymal stem cells (rBMSCs) and human umbilical vein endothelial cells (HUVECs) were cultured and tested in vitro to evaluate biocompatibility, cell adhesion, proliferation, and pro-osteogenic and pro-angiogenic activity. In vivo experiments were conducted in the rat tibial model of distraction osteogenesis.The developed nanocomposite hydrogels exhibited excellent injectability, robust bone adhesion, superior stretchability, and enhanced osteogenic activity. The results of in vitro and in vivo studies showed that PEGS/ICPN hydrogels could promote new bone formation and mineralization during the dynamic distraction process through the synergistic effects of angiogenesis and osteogenesis.This periosteum-inspired nanocomposite hydrogel represents a mechanobiology approach for effectively restoring large bone defects through the dynamic DO process.
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Bai, Rui,Sen, Aritro,Yu, Zhihui,Yang, Gang,Wang, Haidong,Fan, Ruiwen,Lv, Lihua,Lee, Kyung-Bon,Smith, George W,Dong, Changsheng Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.4
The objective of the present studies was to develop and validate a system for isolation, purification and extended culture of pigment-producing cells in alpaca skin (melanocytes) responsible for coat color and to determine the effect of alpha melanocyte stimulating hormone treatment on mRNA expression for the melanocortin 1 receptor, a key gene involved in coat color regulation in other species. Skin punch biopsies were harvested from the dorsal region of 1-3 yr old alpacas and three different enzyme digestion methods were evaluated for effects on yield of viable cells and attachment in vitro. Greatest cell yields and attachment were obtained following dispersion with dispase II relative to trypsin and trypsin-EDTA treatment. Culture of cells in medium supplemented with basic fibroblast growth factor, bovine pituitary extract, hydrocortisone, insulin, 12-O-tetradecanolphorbol-13-acetate and cholera toxin yielded highly pure populations of melanocytes by passage 3 as confirmed by detection of tyrosinase activity and immunocytochemical localization of melanocyte markers including tyrosinase, S-100 and micropthalmia-associated transcription factor. Abundance of mRNA for tyrosinase, a key enzyme in melanocyte pigment production, was maintained through 10 passages showing preservation of melanocyte phenotypic characteristics with extended culture. To determine hormonal responsiveness of cultured melanocytes and investigate regulation of melanocortin 1 receptor expression, cultured melanocytes were treated with increasing concentrations of ${\alpha}$-melanocyte stimulating hormone. Treatment with ${\alpha}$-melanocyte stimulating hormone increased melanocortin receptor 1 mRNA in a dose dependent fashion. The results demonstrated culture of pure populations of alpaca melanocytes to 10 passages and illustrate the potential utility of such cells for studies of intrinsic and extrinsic regulation of genes controlling pigmentation and coat color in fiber-producing species.
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