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        In silico genome wide identification and expression analysis of the WUSCHEL-related homeobox gene family in Medicago sativa

        Yang, Tianhui,Gao, Ting,Wang, Chuang,Wang, Xiaochun,Chen, Caijin,Tian, Mei,Yang, Weidi Korea Genome Organization 2022 Genomics & informatics Vol.20 No.2

        Alfalfa (Medicago sativa) is an important food and feed crop which rich in mineral sources. The WUSCHEL-related homeobox (WOX) gene family plays important roles in plant development and identification of putative gene families, their structure, and potential functions is a primary step for not only understanding the genetic mechanisms behind various biological process but also for genetic improvement. A variety of computational tools, including MAFFT, HMMER, hidden Markov models, Pfam, SMART, MEGA, ProtTest, BLASTn, and BRAD, among others, were used. We identified 34 MsWOX genes based on a systematic analysis of the alfalfa plant genome spread in eight chromosomes. This is an expansion of the gene family which we attribute to observed chromosomal duplications. Sequence alignment analysis revealed 61 conserved proteins containing a homeodomain. Phylogenetic study sung reveal five evolutionary clades with 15 motif distributions. Gene structure analysis reveals various exon, intron, and untranslated structures which are consistent in genes from similar clades. Functional analysis prediction of promoter regions reveals various transcription binding sites containing key growth, development, and stress-responsive transcription factor families such as MYB, ERF, AP2, and NAC which are spread across the genes. Most of the genes are predicted to be in the nucleus. Also, there are duplication events in some genes which explain the expansion of the family. The present research provides a clue on the potential roles of MsWOX family genes that will be useful for further understanding their functional roles in alfalfa plants.

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        A reduced time-varying model for a long beam on elastic foundation under moving loads

        Guiming Mei,Caijin Yang,Shulin Liang,Jiangwen Wang,Dong Zou,Weihua Zhang,Yunshi Zhao,Zhong Huang,Shuqi Song,Mengying Tan,Yao Cheng,Bingrong Miao 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.9

        Dynamics of a long beam on the elastic foundation subjected to moving loads is studied in the present paper. The sliding window technique is used to dynamically truncate the long beam and a reduced time-varying beam system is obtained. The Hamilton’s principle is employed to establish the equations of motion of the reduced system. The variable separation method is adopted to solve dynamical responses of the reduced system. Examples of a long simply supported Timoshenko beam on the nonlinear foundation subjected to a single moving load and multiple loads are included. Numerical results of the reduced model compared with the ones obtained from the moving element model adapted in literature are carried out to show the validity and the good efficiency of the method proposed in the present paper.

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