http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Cabulong, R.B.,Valdehuesa, K.N.G.,Ramos, K.R.M.,Nisola, G.M.,Lee, W.K.,Lee, C.R.,Chung, W.J. IPC Science and Technology Press 2017 Enzyme and microbial technology Vol.97 No.-
<P>The microbial production of renewable ethylene glycol (EG) has been gaining attention recently due to its growing importance in chemical and polymer industries. EG has been successfully produced biosynthetically from D-xylose through several novel pathways. The first report on EG biosynthesis employed the Dahms pathway in Escherichia coli wherein 71% of the theoretical yield was achieved. This report further improved the EG yield by implementing metabolic engineering strategies. First, D-xylonic acid accumulation was reduced by employing a weak promoter which provided a tighter control over Xdh expression. Second, EG yield was further improved by expressing the YjgB, which was identified as the most suitable aldehyde reductase endogenous to E. coli. Finally, cellular growth, D-xylose consumption, and EG yield were further increased by blocking a competing reaction. The final strain (WTXB) was able to reach up to 98% of the theoretical yield (25% higher as compared to the first study), the highest reported value for EG production from D-xylose. (C) 2016 Elsevier Inc. All rights reserved.</P>
Cabulong, Rhudith B.,Lee, Won-Keun,Bañ,ares, Angelo B.,Ramos, Kristine Rose M.,Nisola, Grace M.,Valdehuesa, Kris Niñ,o G.,Chung, Wook-Jin Springer-Verlag 2018 Applied microbiology and biotechnology Vol.102 No.5
<P>Glycolic acid (GA) is an ai-hydroxy acid used in cosmetics, packaging, and medical industries due to its excellent properties, especially in its polymeric form. In this study, Escherichia coli was engineered to produce GA from D-xylose by linking the Dahms pathway, the glyoxylate bypass, and the partial reverse glyoxylate pathway (RGP). Initially, a GA-producing strain was constructed by disrupting the xylAB and glcD genes in the E. coli genome and overexpressing the xdh(Cc) from Caulobacter crescentus. This strain was further improved through modular optimization of the Dahms pathway and the glyoxylate bypass. Results for module 1 showed that the rate-limiting step of the Dahms pathway was the xylonate dehydratase reaction, and the overexpression of yagF was sufficient to overcome this bottleneck. Furthermore, the appropriate aldolase gene for module 1 was proven to be yagE. The results also show that overexpression of the lactaldehyde dehydrogenase gene, aldA, is needed to increase the GA production while the overexpression of glyoxylate reductase gene, ycdW, was only essential when the glyoxylate bypass was active. On the other hand, the module 2 enzymes AceA and AceK were vital in activating the glyoxylate bypass, while the RGP enzymes were dispensable. The final strain (GA19) produced 4.57 g/L GA with a yield of 0.46 g/g from D-xylose. So far, this is the highest value achieved for GA production in engineered E. coli through the Dahms pathway.</P>
Metabolic Engineering for higher yield of ethylene glycol production inEscherichia coli
Rhudith B. CABULONG,Kris Nino G. VALDEHUESA,Kristine Rose M. RAMOS,Perry Ayn Mayson A. MAZA,Jester O. PANGAN,Angelo B. BAnARES,Grace M. NISOLA,Seong-Poong LEE,Won-Keun LEE,Chang Ro LEE,Wook-Jin CHUNG 한국생물공학회 2016 한국생물공학회 학술대회 Vol.2016 No.4