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- 저자 : Cabrera, M.R. (검색결과 105 건)
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Cabrera, S.,Ji, A.R.,Frejo, L.,Ramos-Mejia, V.,Romero, T.,Real, P.,Lopez-Escamez, J.A. Elsevier 2015 Stem cell research Vol.15 No.2
We have generated iPSCs from peripheral blood mononuclear cells (PBMCs) of a healthy man using heat sensitive and non-integrative Sendai virus containing Sox2, Oct¾, c-Myc and Klf4. Human GRX-MCiPS4F-A2 cell line was established and characterized through this study.
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On design criteria for a disconnectable FPSO mooring system associated with expected life-cycle cost
Cabrera-Miranda, José,Manuel,Sakugawa, Patrí,cia Mika,Corona-Tapia, Rafael,Paik, Jeom Kee Informa UK (TaylorFrancis) 2018 SHIPS AND OFFSHORE STRUCTURES Vol.13 No.4
<P>Some floating production, storage and offloading units (FPSOs) possess disconnectable systems to avoid harsh environments. According to a literature survey, the practice is based on perceptions and experiences of operators to judge disconnection; however, this paper offers a rational approach. A life-cycle cost model is proposed to optimise (1) the disconnection criteria and (2) the design of mooring lines under reliability format. Relevant ultimate limit states are considered in association with hull, moorings and green water failure. Effects of future failure costs are considered (downtime, environmental damage, reputation, etc.). Disconnection criteria are then formulated in terms of significant wave height and wind speed limits. Because a permanent mooring system may exhibit excessive resistance, it is possible to reduce the lines' thickness until the cost is optimised for non-permanent service. Results for an example in the Gulf of Mexico show that important savings can be achieved by implementing the proposed optimisations.</P>
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Cabrera-Miranda, José,Manuel,Paik, Jeom Kee Elsevier 2018 Ocean engineering Vol.149 No.-
<P><B>Abstract</B></P> <P>A top-tensioned riser is a slender pipe that conveys fluids between a floater and a subsea system. High top-tension keeps its straight configuration and helps to prevent compressive loads. Because of the floater's heave motion, the tension on the riser fluctuates giving rise to dynamic buckling. This paper examines the dynamic buckling characteristics of a top-tensioned riser analyzing the governing equation with nonlinear damping. The equation is discretized in space by the finite difference method and then is numerically integrated by the Runge-Kutta method. As main objective, an ultimate limit state function for risers is used to investigate its reliability during parametric excitation. While the short-term stationary Gaussian random motion of a floater can be described by a response spectrum, the uncertainties of a long-term response are considered by Monte Carlo simulation. In view of an applied example, it is found that the dynamic buckling would occur often, and although the probability of failure is acceptable, it can cause serious failure when axial excitation is of significance in harsher sea states. This study aims to contribute in clarifying the role of parametric vibrations (dynamic buckling) in the reliability of risers for ultimate limit state.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Dynamic buckling of a top-tensioned riser in floating structures is studied. </LI> <LI> The effect of dynamic buckling on the ultimate limit state based reliability of a riser is also studied. </LI> <LI> The nonlinear governing differential equation is solved by Runge-Kutta method. </LI> <LI> The uncertainties of a long-term response are considered by Monte Carlo simulation. </LI> <LI> The dynamic buckling can cause serious failure of a riser in harsher sea states. </LI> </UL> </P>
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Effects of Microalgae and Salinity on the Growth of Three Types of the Rotifer Brachionus plicatilis
Cabrera Tomas,Bae Jean Hee,Bai Sungchul C.,Hur Sung Bum The Korean Society of Fisheries and Aquatic Scienc 2005 Fisheries and Aquatic Sciences Vol.8 No.2
We investigated the effects of salinity and three food species of microalgae on the growth of three types of the rotifer Brachionus plicatilis, with the aim of improving mass culture of rotifers in hatcheries. Three types (large, small, and ultra-small) of the rotifer were cultured at 16 ppt and 32 ppt salinity with the green algae Chlorella ellipsoidea, Nannochloris oculata, or Tetraselmis tetrathele. The maximum density and specific growth rate were compared for each rotifer type. Ultra-small rotifers grew significantly faster at 16 ppt salinity than at 32 ppt, and C. ellipsoidea and T. tetrathele promoted significantly higher growth than did N. oculata. However, small rotifers grew significantly better at 32 ppt salinity than at 16 ppt, and small rotifers fed on N. oculata achieved the highest density at 1,185 individuals/ml. Large rotifers grew faster at 16 ppt salinity than at 32 ppt, with a diet of T. tetrathele resulting in the fastest growth. Each type of rotifer thrived under different regimens of microalgae and salinity.
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Cabrera Tomas,Hur Sung Bum The Korean Society of Fisheries and Aquatic Scienc 2005 Fisheries and Aquatic Sciences Vol.8 No.4
Despite the relatively high production of fingerlings of the olive flounder Paralichthys olivaceus, its larval rearing in terms of first feeding has not been fully analyzed. We evaluated the variations of amino acids and fatty acids of starved larvae over 96 hr after hatching. We also investigated depletion of the yolk and oil globule of starved larvae and those fed the rotifer Brachionus plicatilis. In addition, the optimum size of the rotifers according to the mouth size of the larvae, and the point of no return with delay of the first feeding, were also examined. The amino acids in the egg decreased abruptly during embryo development. At 48 to 72 hr after hatching, the amino acids of starved larvae decreased by $30-40\%$ from the level in newly hatched larvae. The concentrations of fatty acids in newly hatched larvae were lower than those of floating eggs and dropped sharply at 48 hr after hatching, when the yolk disappeared. The starved larvae depleted their yolksacs and oil globules earlier than the fed larvae did. At 84 hr after hatching, rotifers were detected for the first time in the guts of the larvae, which were about 3 mm in total length. The point of no return appeared to be close to the fourth day from the first feeding. For a high survival rate of P. olivaceus larvae, the first feeding should occur before the third day after hatching.
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Cabrera Tomas,Bae Jean Hee,Bai Sungchul C.,Hur Sung Bum The Korean Society of Fisheries and Aquatic Scienc 2005 Fisheries and Aquatic Sciences Vol.8 No.4
Microalgae are widely used for mass culture of the rotifer Brachionus plicatilis in aquaculture. Since the nutritional value of the rotifer is closely related to its food, the nutritional value of its food should be known in detail. Chlorella ellipsoidea and Nannochloris oculata are representative food organisms for rotifers that are easily cultured. Therefore, the nutritional values of these micro algae were examined for ultrasmall, small, and large rotifers and Artemia nauplii. Chlorella ellipsoidea contained seven times more total fatty acids than N. oculata. The three types of rotifer fed N. oculata contained more amino acids than those fed C. ellipsoidea. However, the total fatty acids of the rotifers fed each microalga species differed according to the type of rotifer. Newly hatched Artemia nauplii contained more protein and had a higher dry weight than those fed microalgae for 6 h. As with the rotifers, the Artemia nauplii fed N. oculata contained more protein and amino acids than those fed C. ellipsoidea, while the reverse was true for the total fatty acid content. Our results suggest that N. oculata is a good supply of protein, while C. ellipsoidea is a good source of lipids as food organisms for rotifers and Artemia nauplii in aquaculture.
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Cabrera-Miranda, José,Manuel,Paik, Jeom Kee Pergamon Press 2019 Ocean engineering Vol.174 No.-
<P><B>Abstract</B></P> <P>A riser conveys fluids from a subsea system to a host floater; however, oil and gas phases may alternate, increasing pipe's stress and damaging downstream facilities. This paper studies the nonlinear planar vibrations of a steel lazy wave riser excited by slug flow. The employed formulations comprise the Euler-Bernoulli beam model and the steady plug-flow model with a time-space-varying mass per unit length in the form of a rectangular pulse train. The equations are solved by a Runge-Kutta finite difference scheme and frequency-response curves are constructed for effective tension, curvature, usage factor and fatigue damage. The results offer a useful insight of the slugging frequencies and slug lengths that may receive attention during the design of risers.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Influence of two-phase flow induced vibration (2-FIV) on structural limit states for a marine riser. </LI> <LI> The governing equations of motion are solved by Runge-Kutta-finite difference numerical analysis. </LI> <LI> Large amplitude motions are observed at the bottom end of a steel lazy wave riser. </LI> <LI> 2-FIV can consume substantial percentage of the ULS usage factor, and cause serious fatigue damage. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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A multi-country analysis on potential adaptive mechanisms to cold and heat in a changing climate
Vicedo-Cabrera, Ana M.,Sera, Francesco,Guo, Yuming,Chung, Yeonseung,Arbuthnott, Katherine,Tong, Shilu,Tobias, Aurelio,Lavigne, Eric,de Sousa Zanotti Stagliorio Coelho, Micheline,Hilario Nascimento Sal Elsevier 2018 Environment international Vol.111 No.-
<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Temporal variation of temperature-health associations depends on the combination of two pathways: pure adaptation to increasingly warmer temperatures due to climate change, and other attenuation mechanisms due to non-climate factors such as infrastructural changes and improved health care. Disentangling these pathways is critical for assessing climate change impacts and for planning public health and climate policies. We present evidence on this topic by assessing temporal trends in cold- and heat-attributable mortality risks in a multi-country investigation.</P> <P><B>Methods</B></P> <P>Trends in country-specific attributable mortality fractions (AFs) for cold and heat (defined as below/above minimum mortality temperature, respectively) in 305 locations within 10 countries (1985–2012) were estimated using a two-stage time-series design with time-varying distributed lag non-linear models. To separate the contribution of pure adaptation to increasing temperatures and active changes in susceptibility (non-climate driven mechanisms) to heat and cold, we compared observed yearly-AFs with those predicted in two counterfactual scenarios: trends driven by either (1) changes in exposure-response function (assuming a constant temperature distribution), (2) or changes in temperature distribution (assuming constant exposure-response relationships). This comparison provides insights about the potential mechanisms and pace of adaptation in each population.</P> <P><B>Results</B></P> <P>Heat-related AFs decreased in all countries (ranging from 0.45–1.66% to 0.15–0.93%, in the first and last 5-year periods, respectively) except in Australia, Ireland and UK. Different patterns were found for cold (where AFs ranged from 5.57–15.43% to 2.16–8.91%), showing either decreasing (Brazil, Japan, Spain, Australia and Ireland), increasing (USA), or stable trends (Canada, South Korea and UK). Heat-AF trends were mostly driven by changes in exposure-response associations due to modified susceptibility to temperature, whereas no clear patterns were observed for cold.</P> <P><B>Conclusions</B></P> <P>Our findings suggest a decrease in heat-mortality impacts over the past decades, well beyond those expected from a pure adaptation to changes in temperature due to the observed warming. This indicates that there is scope for the development of public health strategies to mitigate heat-related climate change impacts. In contrast, no clear conclusions were found for cold. Further investigations should focus on identification of factors defining these changes in susceptibility.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Evidence on adaptation is crucial for the assessment of climate change impacts. </LI> <LI> Most of the studied countries showed a strong reduction in heat vulnerability. </LI> <LI> We found more diffuse patterns on cold-mortality trends. </LI> <LI> Pace of decrease in heat susceptibility was faster than the observed warming. </LI> <LI> There is scope for development of mitigation strategies of climate change impacts. </LI> </UL> </P>
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