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Allium fistulosum 과 Allium ascalonicum 에서 헤테로크로마틴 분포에 의한 핵형분석
서봉보(Bong Bo Seo),김종호(Jong Ho Kim) 한국식물학회 1975 Journal of Plant Biology Vol.18 No.3
The present study demonstrates karyotype based on H-patterns of A.fistulosum and A. ascalonicum using Giemsa technique. The results obtained in this study are summarized as follows: ⅰ). Karyotypic analysis of A.fistulosum is 6VⅡ+JⅡ^t+JⅡ and that of .A. ascalonicum collected from a local farm in the suberbs of Taegu city clearly heterozygous as 13V+J_1^t+J_2+ⅰ. ⅱ). The heterochromatin of both species is generally located distally in both arms of chromosomes and each chromosome type possesses some variations on H-patterns. ⅲ). The percentage of heterochromatin to total chromosome length in cell is about 14.6% in A. fistulosum, 12.8% in A. ascalonicum. The number of bands is revealed about 38 in A. fistulosum and 33 in A. ascalonicum. Also in the amounts of chromocenters per nucleus, the former is somewhat more than the latter.
닭(gallus domesticus)의 經濟形質 改良을 위한 染色體 分染分析과 標識因子의 究明에 관한 硏究 Ⅰ. 品種間의 比較
Bong Kug Ohh(吳鳳國),Jung Sou Yeo(呂政秀),Bong Bo Seo(徐奉甫) 한국육종학회 1985 한국육종학회지 Vol.17 No.1
This experiment was carried out to identify the differences of genetic materials as genetic marker for the three chick breed. The characteristics of chick chromosome were as follows. The 10 pairs chromosomes generally identified by morphology vary in size from about 7 ㎛ to small dot. It was identified that chromosome #1 and 2 were grouped as sub-metacentric, #3 was telocentric, #4 was as acrocentric and #5 was as metacentric and sex chromosome Z. Residual as intermediate chromosomes is found as acrocentric for #6, #7, #8, #10 and sub-metacentric for #9. Metacentric sex chromosome W was similiar size of the #9. The identification of the egg type, meat type, and dual purpose type chicken was also as follows. Egg type chick was featured by the less band at distal part of #1-p and #4-q than others. Dark band adjacent to centromere of meat type chick was found as the specific marker and in dual purpose chick was more bands at #1-p, #2-p, and central part of #3 than the other breeds.
닭(gallus domesticus)의 經濟形質 改良을 위한 染色體 分染分析과 標識因子의 究明에 관한 硏究 Ⅲ. 交雜種과 純種間의 比較
Bang Bo Seo(徐奉甫),Bong Kug Ohh(吳鳳國),Jung Sou Yeo(呂政秀) 한국육종학회 1985 한국육종학회지 Vol.17 No.1
This experiment was carried out to identify the differences of genetic materials as genetic marker for crossbred of egg type and dual purpose chicken. The characteristics of chromosomes in egg type and dual purpose hybrid were as follows. The differences from the origin of the parent chromosomes were found that chromosome #2-q was non-banded centromere, #4-q was dark band adjacent to centromere as the marker of meat type chick, and Z chramosome was faint dark band adjacent to centromere.
Foliar ultrastructure of Korean Orostachys species
김인선,박재홍,서봉보,송승달,Kim, In-Sun,Pak, Jae-Hong,Seo, Bong-Bo,Song, Seung-Dal Korean Society of Electron Microscopy 1995 Applied microscopy Vol.25 No.4
CAM대사를 수행하는 것으로 추정되는 한국산 바위솔속식물 3종(난장이바위솔, 바위솔, 둥근바위솔)의 엽육조직이 전자현미경적으로 연구되었다. 다육질성의 이들 엽육조직은 주로 세포의 크기가 큰 수분저장 세포들로 구성되어 있고 세포간극이 잘 발달되어 있다. 세포내 또는 세포간극에는 점액성 분비물질들이 분포하였다. 수분저장 세포에는 하나 또는 여러 개의 큰 액포가 있고 이들이 세포체적의 대부분을 차지하여 세포질은 세포벽 주위에서 매우 적게 분산되어 나타났다. 세포질내에는 다수의 엽록체, 미소체, 미토콘드리아, 소포체, 골지체 등의 전형적인 세포소기관외에 엽록체주변에서 관찰되는 세포질의 특이한 분포양상을 비롯하여 세포벽과 분리되어 나타나는 파상(undulation or invagination)의 세포막과 이중막으로 둘러싸인 원형질막 기원소포(plasmalemmasome)및 수초상구조(myelin-like structure), 액포상구조(vacuole-like structure) 또는 낭상구조(bladder-like structure) 등이 발견되었다. 특히 파상의 세포막 및 액포막의 신장으로 형성된 액포상 또는 낭상구조와 엽록체주변 세포질의 특이한 분포양상 등은 주목할 만한 것으로 이들의 특성 및 구조는 CAM대사와 연계되어 자세히 연구되어야 할 것이다. 이와 같은 엽육조직의 구성 및 미세구조적 특성으로 보아 본 바위솔속 식물들은 CAM대사를 수행하는 식물군으로 사료된다. Ultrastructural characteristics were examined with leaves of three species, O. japonicus A. Berger, O. malacophyllus Fisch., and O. sikokianus Owhi that probably have CAM mode. The mesophyll cells of these Orostachys possessed vacuoles with precipitates, myelin-like figures, and plasmalemmasomes, along with typical chloroplasts, microbodies and darkly stained bodies in their thin peripheral cytoplasm. Separation of the plasmalemma from the cell wall, leaving a space between them, was a common phenomenon in these species. A complex array of small to large vacuoles which contain small, membrane-bounded vesicles or vacuole-like structures were frequently found. A well-developed thylakoid system was observed in the chloroplasts and this indicates that the photosynthetic capacity of these mesophyll cells is probably active. A peculiar configuration of cytoplasm, especially around the chloroplasts, was also encountered. The variety of cytoplasmic constituents and vacuoles suggest the water-storing mesophyll cells may be complex in function. Some cellular features detected in this study strongly suggest the possible occurrence of CAM mode in Orostachys species.
Mi Ah Oh,서봉보,Bong Bo Seo 한국식물학회 2012 Journal of Plant Biology Vol.55 No.5
Numerous studies on Achyranthes japonica have been investigated on physiological and pharmacological interests, however, no information of molecular cytogenetic level has been introduced yet, which, in turn, it is very essential to construct the molecular database and polyploidy primarily for any further researches. In this study, full unit of 5S and partial unit of 45S rDNA including two ITS regions were analyzed with chromosomal loci of examined rRNA genes on mitotic chromosomes. From the sequence analysis of rDNA unit, only a few polymorphic sites revealed in both coding and non-coding regions of NTS, ITS 1 and 2 giving an idea that no inter-specific hybrids has been involved in A. japonica as highly conserved specie through the high evolutionary period. To identify the polyploidy of A. japonica which has been unclear due to the very small size and unspecific centromere, FISH analysis was carried out on mitotic chromosomes using analyzed 5S and pTa-71 for 45S rDNA. 2 loci of each 5S and 45S rDNA were confirmed on the short arm of different chromosomes which were assumed to be a pair of each rDNA by a very similar size. Thus, the analyzed sequence of rDNA with low polymorphic rates and the identified loci on a relative size chromosome suggest the polyploidy of A. japonica as highly conserved diploid specie.