http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Bacillus subtilis as a Tool for Screening Soil Metagenomic Libraries for Antimicrobial Activities
( Biver Sophie ),( Sebastien Steels ),( Daniel Portetelle ),( Micheline Vandenbol ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.6
Finding new antimicrobial activities by functional metagenomics has been shown to depend on the heterologous host used to express the foreign DNA. Therefore, efforts are devoted to developing new tools for constructing metagenomic libraries in shuttle vectors replicatable in phylogenetically distinct hosts. Here we evaluated the use of the Escherichia coli-Bacillus subtilis shuttle vector pHT01 to construct a forest-soil metagenomic library. This library was screened in both hosts for antimicrobial activities against four opportunistic bacteria: Proteus vulgaris, Bacillus cereus, Staphylococcus epidermidis, and Micrococcus luteus. A new antibacterial activity against B. cereus was found upon screening in B. subtilis. The new antimicrobial agent, sensitive to proteinase K, was not active when the corresponding DNA fragment was expressed in E. coli. Our results validate the use of pHT01 as a shuttle vector and B. subtilis as a host to isolate new activities by functional metagenomics.
<i>EPOXI</i> : COMET 103P/HARTLEY 2 OBSERVATIONS FROM A WORLDWIDE CAMPAIGN
Meech, K. J.,A’Hearn, M. F.,Adams, J. A.,Bacci, P.,Bai, J.,Barrera, L.,Battelino, M.,Bauer, J. M.,Becklin, E.,Bhatt, B.,Biver, N.,Bockelé,e-Morvan, D.,Bodewits, D.,Bö,hnhardt, H.,Boissier, J IOP Publishing 2011 ASTROPHYSICAL JOURNAL LETTERS - Vol.734 No.1
<P>Earth- and space-based observations provide synergistic information for space mission encounters by providing data over longer timescales, at different wavelengths and using techniques that are impossible with an in situ flyby. We report here such observations in support of the EPOXI spacecraft flyby of comet 103P/Hartley 2. The nucleus is small and dark, and exhibited a very rapidly changing rotation period. Prior to the onset of activity, the period was similar to 16.4 hr. Starting in 2010 August the period changed from 16.6 hr to near 19 hr in December. With respect to dust composition, most volatiles and carbon and nitrogen isotope ratios, the comet is similar to other Jupiter-family comets. What is unusual is the dominance of CO2-driven activity near perihelion, which likely persists out to aphelion. Near perihelion the comet nucleus was surrounded by a large halo of water-ice grains that contributed significantly to the total water production.</P>