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Dong Sun,Shuyong Jiang,Xiaodong Xing,Bingyao Yan,Junbo Yu,Yanqiu Zhang 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.12
Local canning compression is imposed on equiatomic NiTi shape memory alloy (SMA) with complete B19′ martensite. Afraction of retained nanocrytalline grains are embedded in the dominant amorphous phase. The compressed NiTi samples areannealed for 2 h at various temperatures, including 300, 450 and 600 °C. Grain size increases with increasing crystallizationtemperature during heat treatment. Under annealing at 300 °C, nanocrystalline grains are dominant in NiTi SMA, where thereexists local amorphous zone. Under annealing at 450 °C, almost complete nanocrystalline can be obtained. Under annealingat 600 °C, grain size increases substantially, but there still exists a small amount of nanocrystalline grains. Under annealingat 300 °C, NiTi SMA exhibits extremely high elastic limit, but its plasticity is poor. Under annealing at 450 °C, NiTi SMAexhibits very high yield stress and it simultaneously keeps high plasticity. Under annealing at 600 °C, NiTi SMA exhibitsrelatively low yield stress, but its yield stress is still higher than that of as-received NiTi SMA and its plasticity is also high.
Rapid, large-scale generation of Ds transposant lines and analysis of the Ds insertion sites in rice
Kim, Chul-Min,Piao, Hai-Long,Park, Soon-Ju,Chon, Nam-Soo,Je, Byoung-Il,Bingyao Sun,Park, Sung-Han,Park, Jin-Young,Lee, Eun-Jin,Kim, Min-Jung,Chung, Woo-Sik,Lee, Kon-Ho,Lee, Young-Suk,Lee, Jeung-Joo,Wo Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-
Rapid, large-scale generation of a Ds transposant population was achieved using a regeneration procedure involving tissue culture of seed-derived calli carrying Ac and inactive Ds elements. In the F_(2) progeny from genetic crosses between the same Ds and Ac starter lines, most of the crosses produced an independent germinal transposition frequency of 10-20%. Also, many Ds elements underwent immobilization even though Ac was expressed. By comparison, in a callus-derived regenerated population, over 70% of plants carried independent Ds insertions, indicating transposition early in callus formation. In the remaining population, the majority of plants carried only Ac. Most of the new Ds insertions were stably transmitted to a subsequent generation. An exceptionally high proportion of independent transposants in the regenerated population means that selection markers for transposed Ds and continual monitoring if Ac/Ds activities may not necessarily be required. By analyzing 1297 Ds-flanking DNA sequences, a genetic map of 1072 Ds insertion sites was developed. The map showed that Ds elements were transposed onto all of the rice chromosomes, with preference not only near donor sites (36%) but also on certain physically unlinked arms. Poulations from both genetic crossing and tissue culture showed the same distribution patterns of Ds insertion sites. The information of these mapped Ds insertion sites was deposited in GenBank. Among them, 55% of Ds elements were on predicted open-reading frame (ORF) regions. Thus, we propose an optimal strategy for the rapid generation of a large population of Ds transposants in rice.