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- 저자 : Bibi Zareena (검색결과 1 건)
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Adeeba Khadim,Bibi Zareena,Saddam Hussain,Syed Usama Yaseen Jeelani,Arslan Ali,Syed Ghulam Musharraf 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.107 No.-
High-throughput and efficient identification of drugs in a complex mixture is required in many pharmaceuticalindustries and drug testing laboratories. The present study aims to utilize an efficient poolingstrategy for the preparation of high-resolution tandem mass spectrometry (HR-MS/MS) library of drugswith different therapeutic properties. Compounds were classified into four classes of low, medium–low,medium–high and high log P values and were pooled into five pools (100 drugs pool). Compounds withisobaric and close log p values were placed in separate groups to overcome the co-elution problem. Liquidchromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) was utilized to generate ahigh-resolution tandem mass spectrometry (MS/MS) spectral library in both positive and negative electrosprayionization modes of 491 drugs. Spectra were collected by LC-MS/MS analysis using system automatedcollision energy i.e. of 25–60 eV and four predetermined collision energies (10, 20, 30 and 40 eV)for each compound using schedule precursor list of [M + H]+ and [M + Na]+ ions. To validate the applicabilityof the library, serum samples spiked at three concentrations close to bioavailability (0.308 ng/mL,3.08 ng/mL and 30.08 ng/mL) of drugs and were analyzed by LC-QTOF-MS/MS. The HRMS library searchsuccessfully identified all compounds present in the spiked serum samples based on exact masses of precursorions, MS/MS data, and retention time etc. The accurate mass LC-QTOF-MS based tandem massspectral libraries represent a useful tool for the identification of drugs in clinical samples, for pharmacologicaland forensic screening applications etc.
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