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RISS 인기검색어
- 검색키워드
- 저자 : Bharat B. Sharma (검색결과 3 건)
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Paswan, Chandan,Bhushan, Bharat,Patra, B.N.,Kumar, Pushpendra,Sharma, Arjava,Dandapat, S.,Tomar, A.K.S.,Dutt, Triveni Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.9
The present investigation was undertaken to study the genetic polymorphism of the DRB3 exon 2 in 75 crossbred cattle by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Five genotypes i.e. HaeIII-a, HaeIII-b, HaeIII-e, HaeIII-ab and HaeIII-ae were observed when the 284 bp PCR products were digested with HaeIII restriction enzyme. The corresponding frequencies of these patterns were 0.53, 0.04, 0.01, 0.38 and 0.04, respectively. Digestion with RsaI restriction enzyme resolved 24 different restriction patterns. The frequencies of these patterns ranged from 0.013 (RsaI-f, RsaI-k and RsaI-c/n) to 0.120 (RsaI-n). The results revealed that the crossbred cows belonged to the RsaI patterns namely b, k, l, a/l, d/s, l/n, l/o and m/n, whose corresponding frequencies were 0.027, 0.013, 0.040, 0.027, 0.040, 0.067, 0.027 and 0.067, respectively. Digestion of the 284 bp PCR product of DRB3.2 gene with PstI in the crossbred cattle did not reveal any restriction site. These results suggested the absence of the recognition site in some of the animals. These results also revealed that the crossbred cows studied were in homozygous as well as heterozygous condition. On the basis of the above results it can be concluded that the DRB3.2 gene was found to be highly polymorphic in the crossbred cattle population.
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Saifi, H.W.,Bhushan, Bharat,Kumar, Sanjeev,Kumar, Pushpendra,Patra, B.N.,Sharma, Arjava Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.5
Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) analysis was carried out with a battery of 11 random decamer primers to study band frequency (BF), genetic identity index (I) and mean average percentage difference (MAPD) between Bhadawari and Murrah breeds of buffalo. The primers OPA04 and BG15 resolved a band of 460 bp, which was present only in animals of Bhadawari breed. Whereas, the primers OPA14, BG27 and BG28 produced Murrah specific fragments of sizes 730 bp and 1,230 bp, respectively. The estimate of genetic identity index was highest (0.845) with the primer OPA01 and the lowest (0.479) with the primer BG27. The genetic identity index pooled over the primers was 0.596${\pm}$0.037 between these two breeds. The highest MAPD estimate (53.9) between the two breeds was obtained with the primer BG27 and the lowest (14.3) with the primer OPA01. It might be concluded that the genetic identity index between these two breeds calculated on the basis of BF showed moderate level of genetic identity with the primers employed. MAPD calculated on the basis of uncommon bands also demonstrated lower to medium level of genetic difference between Bhadawari and Murrah breeds of buffalo.
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Direct numerical simulation of evaporation in a biporous media
Himadri Chattopadhyay,Sudip K. Samanta,Gautam Biswas,Bharat B. Sharma 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.6
Biporous media having clusters of size range 300 - 425 µm, 425 - 600 µm, > 600 µm have been sintered at 950 °C , 180 min using gas atomized spherical particles (D50 of 90 microns) of pure copper in reducing atmosphere of pure hydrogen gas. MicroCT scan of biporous samples have been performed to and then images have been reconstructed to 3D CAD models, which further have been discretised into tetrahedral mesh using image processing and reconstruction software Avizo Fire 8.0. Direct numerical simulations have been performed to obtain Evaporative heat transfer coefficient (EHTC) over the surface of these biporous clusters. The transient evaporation phenomena and effect of cluster size on evaporation has been investigated in this paper. These results have been compared with the experimental results available in literature for evaporative heat transfer coefficient for biporous media.
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