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- 저자 : BernhardO.Palsson (검색결과 2 건)
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CraigR.Halberstadt,BernhardO.Palsson,A.ReesMidgley 한국생물공학회 2002 Biotechnology and Bioprocess Engineering Vol.7 No.3
This report describes the use of a transtubular bioreactor to study the relative efectsof diffusion versus perfusion of medium on antibody production by a hybridoma cel line. The study was performed with a high-density cel culture maintained in a serum-fre, low-protein me-dium for 77 days. It was determined that the reactor possessed a macro-mixing pattern residence rangement of the medium lines in the reactor, the flow paterns for nutrient distribution consist of largely independent medium path lengths ranging from short to long. When operated with cy-clic, reversing, transtubular medium flow, some regions of the reactor (with short residence times) are more accesible to medium than others (with long residence times). From this standpoint, the reactor can be divided into three regions: a captive volume, which consists of medium primarily delivered via diffusion; a laped volume, which provides nutrients through unilateral convection; volumes were modified experimentally by changing the period over which the direction of me-dium flow was reversed from 15 min (larger captive volume) to 9 h (larger swept volume). The re-sults suggest that antibody concentration increases as the size of the diffusion-limited (captive) volume is increased to a maximum at around 30 min with a sharp decrease thereafter. As reflected by changes in measured consumption of glucose and production of lactate, no significant difer-ence in celular metabolism occured as the reactor was moved betwen these diferent states. tibody productivity under serum-free, low-protein conditions with minimal effects on celular me-tabolism.
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Characterization of the KG1a Cell Line for Use in a Cell Migration Based Screening Assay
KarlFrancis,이균민,BernhardO.Palsson 한국생물공학회 2002 Biotechnology and Bioprocess Engineering Vol.7 No.3
for potentially therapeutic compounds. Further screening of these lead compounds is typicaly done with secondary assays which may utilize living, functioning cels as screening tools. A prob-lem (or benefit) with these cell-based assays is that living cells are very sensitive to their environ-ment. We have been interested in the process of stem cel migration and how it relates to the cel-lular therapy of bone marrow transplantation. In this study we describe a secondary, cel-based as-say for screening the effects of various in-vitro conditions on Imature Hematopoietic Cell (IHC) or the effect of a cultures growth phase, that need to be accounted for in a screning protocol. Fi-nally, we show that exponentially growing KG1a cells (a human IHC cell line) were 10 times more motile than those in the lag or stationary phases. These data strongly sugest that KG1a cels secrete a chemokinetic factor during the exponential growth phase of a culture.
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