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      • KCI등재

        Prevalence and Molecular Characterization of Methicillin-Resistant Staphylococcus aureus from Nasal Specimens: Overcoming MRSA with Silver Nanoparticles and Their Applications

        Abo-Amer Aly E.,Gad El-Rab Sanaa M. F.,Halawani Eman M.,Niaz Ameen M.,Bamaga Mohammed S. 한국미생물·생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.12

        Staphylococcus aureus is a cause of high mortality in humans and therefore it is necessary to prevent its transmission and reduce infections. Our goals in this research were to investigate the frequency of methicillin-resistant S. aureus (MRSA) in Taif, Saudi Arabia, and assess the relationship between the phenotypic antimicrobial sensitivity patterns and the genes responsible for resistance. In addition, we examined the antimicrobial efficiency and application of silver nanoparticles (AgNPs) against MRSA isolates. Seventy-two nasal swabs were taken from patients; MRSA was cultivated on Mannitol Salt Agar supplemented with methicillin, and 16S rRNA sequencing was conducted in addition to morphological and biochemical identification. Specific resistance genes such as ermAC, aacA-aphD, tetKM, vatABC and mecA were PCR-amplified and resistance plasmids were also investigated. The MRSA incidence was ~49 % among the 72 S. aureus isolates and all MRSA strains were resistant to oxacillin, penicillin, and cefoxitin. However, vancomycin, linezolid, teicoplanin, mupirocin, and rifampicin were effective against 100% of MRSA strains. About 61% of MRSA strains exhibited multidrug resistance and were resistant to 3-12 antimicrobial medications (MDR). Methicillin resistance gene mecA was presented in all MDR-MRSA strains. Most MDR-MRSA contained a plasmid of > 10 kb. To overcome bacterial resistance, AgNPs were applied and displayed high antimicrobial activity and synergistic effect with penicillin. Our findings may help establish programs to control bacterial spread in communities as AgNPs appeared to exert a synergistic effect with penicillin to control bacterial resistance.

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        Palm Oil Fuel Ash: Promising Supplementary Cementing Materials

        S. O. Bamaga,M. W. Hussin,Mohamed A. Ismail 대한토목학회 2013 KSCE JOURNAL OF CIVIL ENGINEERING Vol.17 No.7

        Palm Oil Fuel Ash (POFA) is by-product obtained by burning of fibers, shells and empty fruit bunches as fuel in palm oil mill boilers. In this investigation, three ashes were collected from different palm oil mills around Malaysia and namely CAPOFA,ALPOFA and KTPOFA. The ashes were ground to 45 μm before replace 20% by weight of cement in concrete and mortar. The compressive strength of concretes containing POFA was tested at ages of 7, 28 and 90 days. For durability aspects, concretes and mortars were prepared to investigate the chloride and sulfate resistance respectively in accordance with appropriate ASTM standards. Rapid Chloride Penetration Test (RCPT) was conducted in accordance with ASTM C1202 to investigate the ability of concretes containing POFA to resist the penetration of chloride ions. Change in length and microstructure study for mortar bars containing POFA immersed in sodium sulfate were conducted to evaluate the effects of sulfate attack on POFA mortars. Concrete and mortar specimens were prepared using plain portland cement in order to use as control specimens. At age of 90 days, the results of compressive strength of all POFA concretes were higher than control concrete. All concretes containing POFA showed higher potential to resist chloride ions penetration compared to control concrete. All mortar bars containing POFA showed lower expansion and less porous structure than control mortar. Depending on the results of this investigation, it could be concluded that POFA could be successfully used as supplementary cementing materials to replace 20% of cement in concrete and mortar.

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