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Abhishek Mukherjee,Nupur Sarkar,Anandamay Barik 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.3
Extraction, thin layer chromatography, and gas chromatography–mass spectrometry of young, mature, andsenescent leaves of Momordica cochinchinensis Spreng revealed 13 free fatty acids, representing a total of82.29, 91.30, and 68.52% of fatty acids in young, mature, and senescent leaves, respectively. Palmitic acid wasthe predominant fatty acid followed by stearic acid in three types of leaves. The free fatty acids from young,mature, and senescent leaves attracted female Aulacophora foveicollis Lucas (Coleoptera: Chrysomelidae) at theminimal concentrations of 4, 2, and 8 μg, respectively; whereas the mixtures of synthetic fatty acids mimickingfree fatty acids of young, mature, and senescent leaves showed attraction at the minimal concentrations of 4, 2,and 10 μg, respectively, in Y-shaped glass tube olfactometer bioassay under laboratory condition. The resultsindicate that A. foveicollismay employ long-chain free fatty acids as an olfactory cue for host location. The individualsynthetic fatty acids mimicking the proportions detected in three types of leaves were also evaluated througholfactometer bioassay. Only synthetic palmitic acid at theminimal amount of 2.17 μg attracted the insect. A syntheticblend of fatty acids mimicking 8 μg free fatty acid concentration of mature leaves or an amount of 5.42 μg palmiticacid produced the highest attraction of the insect. Hence 5.42 μg palmitic acid might be used for insect pestmanagementprogram such as baited traps.
Anamika Koner,Rahul Debnath,Anandamay Barik 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.4
Rumex dentatus L. and Polygonum glabrum Willd. (Polygonaceae) are important weeds of wheat and rice fields, respectively, in India and Pakistan. Adults and larvae of Galerucella placida Baly (Coleoptera: Chrysomelidae) feed both weeds. Therefore, we studied the biology of G. placida on two weed leaves using age-stage, two-sex life table at 21 °C, 65 ± 5% r.h. and 12L: 12D. We also studied food utilization indices of G. placida on these leaves. Further, we investigated the quantities of nutrients (total carbohydrates, proteins, lipids, amino acids and nitrogen) and antinutrients (total phenols, flavonols and tannins) of the leaves. Correlation analysis suggested that fecundity and the intrinsic rate of increase (r m ) of G. placida were positively correlated with nutrients of leaves, while negatively correlated with antinutrients. Faster larval development, higher longevity of adults and greater fecundity of G. placida were recorded on R. dentatus than P. glabrum. The r m was higher on R. dentatus than P. glabrum. The nutrients were greater in R. dentatus than P. glabrum, while antinutrients were lower in R. dentatus than P. glabrum. The growth rate and consumption index were greater on R. dentatus than P. glabrum. The above results suggested that better nutritional quality of R. dentatus than P. glabrum influenced faster larval development, higher longevity of adults and greater fecundity of G. placida on R. dentatus. This study concluded that R. dentatus could be used for mass rearing of G. placida in biocontol program prior to release in fields for control of both these weeds.