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RISS 인기검색어
- 검색키워드
- 저자 : An-Xin Ye (검색결과 3 건)
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A Fuzzy C-Means Clustering Algorithm Based on Improved Quantum Genetic Algorithm
An-Xin Ye,Yong-Xian Jin 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.1
Aiming at the problem of traditional fuzzy C-means clustering algorithm that it is sensitive to the initial clustering centers and easy to fall into the local optimization, an improved algorithm that combines Improved Quantum Genetic Optimization with FCM algorithm is proposed. In this study, chromosomes are comprised of quantum bits encoded by real number. Chromosomes are renovated by quantum rotating gates and mutated by quantum hadamard gate. The gradients of object function are utilized in adjusting the value of rotating angle by a dynamic strategy. Each chain of genes represents a optimization result, Therefore, a double searching space is acquired for the same number of chromosomes. Experimental results show that the proposed method improves the stability and the accuracy of classification.
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Identification of a Cancer Stem-like Population in the Lewis Lung Cancer Cell Line
Zhang, An-Mei,Fan, Ye,Yao, Quan,Ma, Hu,Lin, Sheng,Zhu, Cong-Hui,Wang, Xin-Xin,Liu, Jia,Zhu, Bo,Sun, Jian-Guo,Chen, Zheng-Tang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3
Objective: Although various human cancer stem cells (CSCs) have been defined, their applications are restricted to immunocompromised models. Developing a novel CSC model which could be used in immunocompetent or transgenic mice is essential for further understanding of the biomolecular characteristics of tumor stem cells. Therefore, in this study, we analyzed murine lung cancer cells for the presence of CSCs. Methods: Side population (SP) cells were isolated by fluorescence activated cell sorting, followed by serum-free medium (SFM) culture, using Lewis lung carcinoma cell (LLC) line. The self-renewal, differentiated progeny, chemosensitivity, and tumorigenic properties in SP and non-SP cells were investigated through in vitro culture and in vivo serial transplantation. Differential expression profiles of stem cell markers were examined by RT-PCR. Results: The SP cell fraction comprised 1.1% of the total LLC population. SP cells were available to grow in SFM, and had significantly enhanced capacity for cell proliferation and colony formation. They were also more resistant to cisplatin in comparison to non-SP cells, and displayed increased tumorigenic ability. Moreover, SP cells showed higher mRNA expression of Oct-4, ABCG2, and CD44. Conclusion: We identified SP cells from a murine lung carcinoma, which possess well-known characteristics of CSCs. Our study established a useful model that should allow investigation of the biological features and pharmacosensitivity of lung CSCs, both in vitro and in syngeneic immunocompetent or transgenic/knockout mice.
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Histological Changes of Tissues and Cell Wall of Rice Straw Influenced by Chemical Pretreatments
Wang, Jia-Kun,Chen, Xiao-Lian,Liu, Jian-Xin,Wu, Yue-Ming,Ye, Jun-An Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.6
Sodium hydroxide (SH) or ammonium bicarbonate (AB) were applied to rice straw to investigate the effects on histological change of stem tissue or cell wall before and after in sacco degradation using a scanning electron microscope (SEM) and a transmission electron microscope (TEM). The SEM revealed that, the parenchyma and vascular bundles were distorted by treatment with SH at 30 or 45 g/kg straw dry matter. Faultage between phloem of large vascular bundles and parenchyma occurred with further increasing SH to 60 or 75 g/kg. The cell wall in these stem tissues was crimped when observed by TEM. However, only parenchyma and large vascular tissues were slightly distorted in AB-treated stem. For untreated and AB-treated stems, the initiation of observable ruminal degradation of cell wall was prolonged from 12 h for inner parenchyma to 24 h for sclerenchyma and to 48 h for phloem of small vascular bundles, while the outer epidermis was intact even at 72 h. For SH-treated stem, however, the cell wall from all of the investigated tissues, epidermis, small vascular bundles, sclerenchyma, and parenchyma started to be degraded at 12 h incubation. These results indicate that SH treatment contracts rice straw stem leading to an improvement in rumen degradation, and that the degradation of SH-treated stem is bilateral from inner and outer surface simultaneously.
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