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Floral Biology and Flowering Phenology of Jatropha Curcas
Amritpal S. Singh,Mukesh P. Patel,Tanmay K. Patel,D. R. Delvadia,Diwaker R. Patel,Nitish Kumar,Subhash Naraynan,Ranbir S. Fougat 강원대학교 산림과학연구소 2010 Journal of Forest Science Vol.26 No.2
Jatropha curcas is an oil bearing species with multiple uses and considerable economic potential as a biofuel plant. Plant flowering and breeding characteristics are important for us to understand the reproduction of plant populations. The present study describes the floral biology and flowering phenology of J. curcas which is a prerequisite for hybridization program for genetic improvement through conventional breeding. The plant produces flowers in dichasial inflorescences. Normally, the flowers are unisexual, and male and female flowers are produced in the same inflorescence. Only a few male flowers are produced in an inflorescence, and fruits are produced only through pollination between different flowers from the same or different plants. This study includes a description of the inflorescence, flower anatomy of both male and female flowers, female : male ratio, pollen : ovule ratio, flowering phenology, pollen viability, stigma receptivity, comparison of selfing methods and a comparison of geitonogamy and xenogamy. This information may be useful in J. curcas breeding programmes.
Floral Biology and Flowering Phenology of Jatropha Curcas
Singh, Amritpal S.,Patel, Mukesh P.,Patel, Tanmay K.,Delvadia, D.R.,Patel, Diwaker R.,Kumar, Nitish,Naraynan, Subhash,Fougat, Ranbir S. Institute of Forest Science 2010 Journal of Forest Science Vol.26 No.2
Jatropha curcas is an oil bearing species with multiple uses and considerable economic potential as a biofuel plant. Plant flowering and breeding characteristics are important for us to understand the reproduction of plant populations. The present study describes the floral biology and flowering phenology of J. curcas which is a prerequisite for hybridization program for genetic improvement through conventional breeding. The plant produces flowers in dichasial inflorescences. Normally, the flowers are unisexual, and male and female flowers are produced in the same inflorescence. Only a few male flowers are produced in an inflorescence, and fruits are produced only through pollination between different flowers from the same or different plants. This study includes a description of the inflorescence, flower anatomy of both male and female flowers, female : male ratio, pollen : ovule ratio, flowering phenology, pollen viability, stigma receptivity, comparison of selfing methods and a comparison of geitonogamy and xenogamy. This information may be useful in J. curcas breeding programmes.
Nitish Kumar,Amritpal S. Singh,Arpan R. Modi,Armi R. Patel,Bhavesh B. Gajera,Narayanan Subhash 강원대학교 산림과학연구소 2010 Journal of Forest Science Vol.26 No.1
Sixteen microsatellite markers (simple sequence repeat (SSR) markers) were employed to examine the genetic stability of 27 randomly chosen date palm (Phoenix dactylifera L.) plants produced through somatic embryogenesis with upto forty two in vitro subcultures. No microsatellite DNA variation was observed among all micropropagated plants. Our results indicate that the micropropagation protocol used for rapid in vitro multiplication is appropriate and suitable for clonal propagation of date palm and corroborated that somatic embryogenesis can also be used as one of the safe modes for production of true-to-type plants of date palm. This is the first report on the use of microsatellite DNA markers to establish the genetic stability in micropropagated date palm plants.
Kumar, Nitish,Singh, Amritpal S.,Modi, Arpan R.,Patel, Armi R.,Gajera, Bhavesh B.,Subhash, Narayanan Institute of Forest Science 2010 Journal of Forest Science Vol.26 No.1
Sixteen microsatellite markers (simple sequence repeat (SSR) markers) were employed to examine the genetic stability of 27 randomly chosen date palm (Phoenix dactylifera L.) plants produced through somatic embryogenesis with upto forty two in vitro subcultures. No microsatellite DNA variation was observed among all micropropagated plants. Our results indicate that the micropropagation protocol used for rapid in vitro multiplication is appropriate and suitable for clonal propagation of date palm and corroborated that somatic embryogenesis can also be used as one of the safe modes for production of true-to-type plants of date palm. This is the first report on the use of microsatellite DNA markers to establish the genetic stability in micropropagated date palm plants.
Agrobacterium tumefaciens-Mediated Genetic Transformation: Mechanism and Factors
Nitish Kumar,K.G. Vijayanand,Muppala P. Reddy,Amritpal S. Singh,Subhash Naraynan 강원대학교 산림과학연구소 2009 Journal of Forest Science Vol.25 No.3
Agrobacterium–mediated genetic transformation has been widely used for the production of genetically modified transgenic plants to obtain specific desired traits. Most of the molecular mechanisms that underlie the transformation steps have been well elucidated over the years. However, a few steps, such as nuclear targeting, T-DNA integration, and Agrobacterium-plant proteins involved remain largely obscure and are still under extensive studies. This review describes the major steps involved in the molecular mechanism of Agrobacterium-mediated transformation and provides insight in the recent developments in studies on the Agrobacterium-mediated genetic transformation system. Some factors affecting the transformation efficiency are also briefly discussed.
Agrobacterium tumefaciens-Mediated Genetic Transformation: Mechanism and Factors
Kumar, Nitish,Vijayanand, K.G.,Reddy, Myppala P.,Singh, Amritpal S.,Naraynan, Subhash Institute of Forest Science 2009 Journal of Forest Science Vol.25 No.3
Agrobacterium-mediated genetic transformation has been widely used for the production of genetically modified transgenic plants to obtain specific desired traits. Most of the molecular mechanisms that underlie the transformation steps have been well elucidated over the years. However, a few steps, such as nuclear targeting, T-DNA integration, and Agrobacterium-plant proteins involved remain largely obscure and are still under extensive studies. This review describes the major steps involved in the molecular mechanism of Agrobacterium-mediated transformation and provides insight in the recent developments in studies on the Agrobacterium-mediated genetic transformation system. Some factors affecting the transformation efficiency are also briefly discussed.