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      • Characterization of Bacillus anthracis proteases through protein-protein interaction: an in silico study of anthrax pathogenicity

        Amrita Banerjee,Pradeep Kumar Das Mohapatra,Shilpee Pal,Keshab Chandra Mondal,Bikash Ranjan Pati,Arnab Sen,Tanmay Paul 셀메드 세포교정의약학회 2014 TANG Vol.4 No.1

        Anthrax is the deadly disease for human being caused by Bacillus anthracis. Instantaneous research work on the mode of infection of the organism revealed that different proteases are involved in different steps of pathogenesis. Present study reports the in silico characterization and the detection of pathogenic proteases involved in anthrax infection through protein-protein interaction. A total of 13 acid, 9 neutral, and 1 alkaline protease of Bacillus anthracis were selected for analysing the physicochemical parameter, the protein superfamily and family search, multiple sequence alignment, phylogenetic tree construction, protein-protein interactions and motif finding. Among the 13 acid proteases, 10 were found as extracellular enzymes that interact with immune inhibitor A (InhA) and help the organism to cross the blood brain barrier during the process of infection. Multiple sequence alignment of above acid proteases revealed the position 368, 489, and 498-contained 100% conserved amino acids which could be used to deactivate the protease. Among the groups analyzed, only acid protease were found to interact with InhA, which indicated that metalloproteases of acid protease group have the capability to develop pathogenesis during B. anthracis infection. Deactivation of conserved amino acid position of germination protease can stop the sporulation and germination of B anthracis cell. The detailed interaction study of neutral and alkaline proteases could also be helpful to design the interaction network for the better understanding of anthrax disease.

      • SCOPUSKCI등재

        Cultivable Bacterial Community Analysis of Dairy Activated Sludge for Value Addition to Dairy Wastewater

        Biswas, Tethi,Chatterjee, Debasmita,Barman, Sinchini,Chakraborty, Amrita,Halder, Nabanita,Banerjee, Srimoyee,Chaudhuri, Shaon Ray The Korean Society for Microbiology and Biotechnol 2019 한국미생물·생명공학회지 Vol.47 No.4

        Analysis of bacterial communities based on their 16S rDNA sequences revealed the predominance of Proteobacteria (Aeromonas sp., Acinetobacter sp. and Thaueraamino aromatica sp.) and uncultured bacterium in activated sludge from the effluent treatment plant (ETP) of Mother Dairy, Calcutta (India). Each isolate was used for bioremediation of dairy wastewater with simultaneous conversion of nitrogenous pollutants into ammonia. A consortium developed using seven of these isolates and three Bacillus strains from different environmental origins could reduce 93% nitrate with simultaneous production of ammonia (626 ㎍/100 ml) within 20 h in non-aerated, immobilized conditions as compared to 82% nitrate reduction producing 2.4 ㎍/100 ml ammonia in 96 h with extensive aeration in a conventional ETP. The treated ammonia-rich effluent could be used instead of freshwater and fertilizer during cultivation of mung bean with 1.6-fold increase in grain yield. The ETP with the surrounding agricultural land makes this process a zero liquid discharge technology for using the biofertilizer generated. In addition, the process requires minimal energy supporting sustained environmental health. This method is thus proposed as an alternative approach for small-scale dairy ETPs.

      • KCI등재

        Cultivable Bacterial Community Analysis of Dairy Activated Sludge for Value Addition to Dairy Wastewater

        Tethi Biswas,Debasmita Chatterjee,Sinchini Barman,Sinchini Barman,Amrita Chakraborty,Nabanita Halder,Srimoyee Banerjee,Shaon Ray Chaudhuri 한국미생물·생명공학회 2019 한국미생물·생명공학회지 Vol.47 No.4

        Analysis of bacterial communities based on their 16S rDNA sequences revealed the predominance of Proteobacteria (Aeromonas sp., Acinetobacter sp. and Thaueraamino aromatica sp.) and uncultured bacterium in activated sludge from the effluent treatment plant (ETP) of Mother Dairy, Calcutta (India). Each isolate was used for bioremediation of dairy wastewater with simultaneous conversion of nitrogenous pollutants into ammonia. A consortium developed using seven of these isolates and three Bacillus strains from different environmental origins could reduce 93% nitrate with simultaneous production of ammonia (626 μg/100 ml) within 20 h in non-aerated, immobilized conditions as compared to 82% nitrate reduction producing 2.4 μg/100 ml ammonia in 96 h with extensive aeration in a conventional ETP. The treated ammonia- rich effluent could be used instead of freshwater and fertilizer during cultivation of mung bean with 1.6-fold increase in grain yield. The ETP with the surrounding agricultural land makes this process a zero liquid discharge technology for using the biofertilizer generated. In addition, the process requires minimal energy supporting sustained environmental health. This method is thus proposed as an alternative approach for small-scale dairy ETPs.

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