http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Passeri, Rosita,Aloisi, Gian Gaetano,Elisei, Fausto,Latterini, Loredana,Caronna, Tullio,Fontana, Francesca,Sora, Isabella Natali Korean Society of Photoscience 2009 Photochemical & photobiological sciences Vol.8 No.11
In this work, three compounds having the same organic moiety (N-methyl-5-azahelicenium salts) but different counterions ($I^-$, $NO_3{^-}$ and $COOCF_3{^-}$) have been investigated in buffered aqueous solutions and in the presence of DNA to give information on the counterion effects on the binding. In particular, the absorption spectra, fluorescence quantum yields and fluorescence lifetimes in aqueous solution for free organic molecules have been determined by steady-state and time-resolved spectrofluorimetric measurements. The obtained values are compared with those of the chromophores in the presence of increasing concentrations of DNA. The results allow determination of the association constants ($K_a$) and the number of base couples per chromophore molecule (n) by means of the McGhee Von Hippel model. The binding parameters are strongly affected by the nature of counterions since the highest $K_a$ value was determined for the compound having $COOCF_3{^-}$; on the other hand the $NO_3{^-}$ derivative is able to interact with the highest number of binding sites. The morphology and structural properties of the DNA-chromophore complexes were investigated by circular dichroism (CD) and atomic force microscopy (AFM). The data revealed that $I^-$ and $COOCF_3{^-}$ derivatives preferentially form intercalation complexes, while the $NO_3{^-}$ salt is able to form intercalation and grove binding complexes at the same time.
Micro and Nanotechnology for Early Diagnosis and Detection of Rheumatic Diseases-molecular Markers
Elisabetta Marulli,Alessandra Aloisi,Paolo Di Giuseppe,Rosaria Rinaldi 한국바이오칩학회 2016 BioChip Journal Vol.10 No.3
Cytokine proteins are known as biomarker molecules, characteristic of a disease or specific body condition. Monitoring of the cytokine pattern in body fluids contributes to the diagnosis of rheumatic diseases associated with rheumatoid factor. Here, the proinflammatory cytokine Tumor Necrosis Factor alpha (TNF-α) was chosen as the first target of interest. We report on the development of a fast sandwich immunosorbent assay: anti-TNF-α antibody (Ab) immobilization on a pre-activated 3D micro-structured glass slide, coupled with direct fluorescence readout method. Two differently functionalized glass - Ab-immobilization substrates - were compared: O2 PLASMA/APTES activated glass - having a two-dimensional structure-, and the polystyrene (PS) beads coated glass, -having a 3D structure-. The 3D structured substrate was aimed to increase antibody anchorage sites. Then, the optimization of buffer media was carefully investigated with respect to non-specific protein binding. As a first step towards real sample analysis, a proof of principle of on/ off cytokine detection has been analyzed in the presence of human serum. High-density one-step immobilization of anti-TNF-α antibody onto nanobead-coated glass slide chip has been demonstrated to be a promising device for application in in vitro diagnostic and profiling of cytokines with potential application toward personalized therapeutic interventions.
LEGACY EXTRAGALACTIC UV SURVEY (LEGUS) WITH THE<i>HUBBLE SPACE TELESCOPE</i>. I. SURVEY DESCRIPTION
Calzetti, D.,Lee, J. C.,Sabbi, E.,Adamo, A.,Smith, L. J.,Andrews, J. E.,Ubeda, L.,Bright, S. N.,Thilker, D.,Aloisi, A.,Brown, T. M.,Chandar, R.,Christian, C.,Cignoni, M.,Clayton, G. C.,Silva, R. da,Mi IOP Publishing 2015 The Astronomical journal Vol.149 No.2